Rabbit Furin Polyclonal Antibody | anti-FURIN antibody

Furin Polyclonal Antibody

Cat. Num. AAA3012725

• Gene Names: FURIN; FUR; PACE; SPC1; PCSK3
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunocytochemistry
• Purity: ProA affinity purified
• Synonyms: Furin; Polyclonal Antibody; Furin Polyclonal Antibody; PACE; FURIN; FUR; PCSK3; Dibasic processing enzyme; Dibasic-processing enzyme; FES upstream region; Furin membrane associated receptor protein; Paired basic amino acid residue cleaving enzyme; Paired basic amino acid residue-cleaving enzyme; Proprotein convertase subtilisin/kexin type 3; SPC1; anti-FURIN antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Furin polyclonal antibody detects endogenous levels of Furin protein.
• Purity/Purification: ProA affinity purified
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 794

• Applicable Applications for anti-FURIN antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)
• Application Notes: WB: 1:500-1:1,000; IHC: 1:50; ICC: 1:50-1:100
• Immunogen: Recombinant protein within human Furin aa 200-400
• Preparation and Storage: Store at 4 degree C after thawing.; Aliquot store at -20 degree C or -80 degree C.; Avoid repeated freeze/thaw cycles.

Western Blot (WB)

(Western Blot analysis of Furin on different cell lysates using anti-Furin antibody at 1/500 dilution. Positive control:Lane 1: HepG2 Lane 2: HelaLane 3: Hela Lane 4: MCF-7)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Furin antibody. Counter stained with hematoxylin.)

Related Product Information for anti-FURIN antibody

Furin (FUR, PACE, PCSK3, SPC1, Kex2p) is a calcium-dependent serine endoprotease that belongs to the subtilisin-like proprotein convertase family. The members of this family process latent precursor proteins into their biologically active products. Furin cleaves at paired basic amino acid processing sites within proparathyroid hormone, transforming growth factor beta 1 precursor, proalbumin, pro-beta-secretase, membrane type-1 matrix metalloproteinase, beta subunit of pro-nerve growth factor and von Willebrand factor. Furin can directly cleave proMMP-2 within the trans-Golgi network leading to an inactive form of matrix metalloproteinase-2 (MMP-2). Furin is synthesized as an inactive zymogen that may minimize the occurrence of premature enzymatic activity that would lead to alternative protein activation or degradation. The inhibitory mechanism is based on the presence of an inactivating prosegment at the NH2 terminal of the Furin. After initial autocatalytic cleavage, the prosegment remains tightly associated until it reaches the trans-Golgi network where the dissociation of the prosegment and activation of furin occurs.

NCBI and Uniprot Product Information

• NCBI GI #: 577019578
• NCBI GeneID: 5045
• NCBI Accession #: NP_001276752.1
• NCBI GenBank Nucleotide #: NP_001276752.1
• UniProt Accession #: P09958
• Molecular Weight: 87kDa
• NCBI Official Full Name: furin preproprotein
• NCBI Official Synonym Full Names: furin, paired basic amino acid cleaving enzyme
• NCBI Official Symbol: FURIN
• NCBI Official Synonym Symbols: FUR; PACE; SPC1; PCSK3
• NCBI Protein Information: furin
• UniProt Protein Name: Furin
• Protein Family: Furin
• UniProt Gene Name: FURIN
• UniProt Synonym Gene Names: FUR; PACE; PCSK3; PACE
• UniProt Entry Name: FURIN_HUMAN

NCBI Description

This gene encodes a member of the subtilisin-like proprotein convertase family, which includes proteases that process protein and peptide precursors trafficking through regulated or constitutive branches of the secretory pathway. It encodes a type 1 membrane bound protease that is expressed in many tissues, including neuroendocrine, liver, gut, and brain. The encoded protein undergoes an initial autocatalytic processing event in the ER and then sorts to the trans-Golgi network through endosomes where a second autocatalytic event takes place and the catalytic activity is acquired. The product of this gene is one of the seven basic amino acid-specific members which cleave their substrates at single or paired basic residues. Some of its substrates include proparathyroid hormone, transforming growth factor beta 1 precursor, proalbumin, pro-beta-secretase, membrane type-1 matrix metalloproteinase, beta subunit of pro-nerve growth factor and von Willebrand factor. It is also thought to be one of the proteases responsible for the activation of HIV envelope glycoproteins gp160 and gp140 and may play a role in tumor progression. This gene is located in close proximity to family member proprotein convertase subtilisin/kexin type 6 and upstream of the FES oncogene. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014]

Uniprot Description

FURIN: Furin is likely to represent the ubiquitous endoprotease activity within constitutive secretory pathways and capable of cleavage at the RX(K/R)R consensus motif. Belongs to the peptidase S8 family. Furin subfamily.

Protein type: Cell surface; EC 3.4.21.75; Membrane protein, integral; Protease; Vesicle

Chromosomal Location of Human Ortholog: 15q26.1

Cellular Component: cell surface; endoplasmic reticulum; extracellular space; Golgi lumen; Golgi membrane; lipid raft; membrane; plasma membrane; trans-Golgi network; trans-Golgi network transport vesicle

Molecular Function: endopeptidase activity; nerve growth factor binding; peptidase activity; peptide binding; protease binding; protein binding; serine-type endopeptidase activity; serine-type endopeptidase inhibitor activity

Biological Process: cell proliferation; cellular protein metabolic process; collagen catabolic process; extracellular matrix disassembly; extracellular matrix organization and biogenesis; negative regulation of low-density lipoprotein receptor catabolic process; negative regulation of nerve growth factor production; negative regulation of transforming growth factor-beta1 production; nerve growth factor processing; nerve growth factor production; peptide biosynthetic process; peptide hormone processing; positive regulation of membrane protein ectodomain proteolysis; protein processing; regulation of endopeptidase activity; regulation of protein catabolic process; secretion by cell; signal peptide processing; transforming growth factor beta receptor signaling pathway; viral infectious cycle; viral protein processing