Goat Frizzled 3 Polyclonal Antibody | anti-FZD3 antibody

Goat anti-Frizzled 3 Antibody

Cat. Num. AAA422104

• Gene Names: FZD3; Fz-3
• Reactivity: Expected from sequence similarity: Human, Mouse, Rat
• Applications: ELISA
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Synonyms: Frizzled 3; Polyclonal Antibody; Goat anti-Frizzled 3 Antibody; FZD3; frizzled homolog 3 (Drosophila); Fz-3; hFz3; frizzled 3; FZD3 antibody; frizzled homolog 3 (Drosophila) antibody; Fz-3 antibody; hFz3 antibody; frizzled 3 antibody; anti-FZD3 antibody

• Host: Goat
• Reactivity: Expected from sequence similarity: Human, Mouse, Rat
• Clonality: Polyclonal
• Purity/Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Form/Format: Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Concentration: 100ug specific antibody in 200ul (varies by lot)
• Sequence: DDQRSKAGSIHSK
• Sequence Length: 666

• Applicable Applications for anti-FZD3 antibody: Peptide ELISA (EIA)
• Application Notes: Peptide ELISA: Antibody detection limit dilution 1: 2000.; Western Blot: Preliminary experiments in Human Brain (Frontal Cortex), Mouse Brain and Rat Brain lysates gave no specific signal but low background (at antibody concentration up to 1ug/ml).
• Immunogen: Peptide with sequence C-DDQRSKAGSIHSK, from the internal region of the protein sequence according to NP_059108.1.
• Epitope: Internal region
• Preparation and Storage: Aliquot and store at -20 degree C. Minimize freezing and thawing.

NCBI and Uniprot Product Information

• NCBI GI #: 8393378
• NCBI GeneID: 7976
• NCBI Accession #: NP_059108.1
• NCBI GenBank Nucleotide #: NM_017412.3
• Molecular Weight: – Da
• NCBI Official Full Name: frizzled-3
• NCBI Official Synonym Full Names: frizzled class receptor 3
• NCBI Official Symbol: FZD3
• NCBI Official Synonym Symbols: Fz-3
• NCBI Protein Information: frizzled-3
• UniProt Protein Name: Frizzled-3
• UniProt Gene Name: FZD3
• UniProt Synonym Gene Names: Fz-3; hFz3
• UniProt Entry Name: FZD3_HUMAN

NCBI Description

This gene is a member of the frizzled gene family. Members of this family encode seven-transmembrane domain proteins that are receptors for the wingless type MMTV integration site family of signaling proteins. Most frizzled receptors are coupled to the beta-catenin canonical signaling pathway. The function of this protein is unknown, although it may play a role in mammalian hair follicle development. Alternative splicing results in multiple transcript variants. This gene is a susceptibility locus for schizophrenia. [provided by RefSeq, Dec 2010]

Uniprot Description

FZD3: Receptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK- 3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues. Belongs to the G-protein coupled receptor Fz/Smo family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Membrane protein, multi-pass; Receptor, GPCR; GPCR, Fz/Smo family

Chromosomal Location of Human Ortholog: 8p21

Cellular Component: apical plasma membrane; axon; cell soma; cell surface; cytoplasm; dendrite; filopodium tip; integral to membrane; lateral plasma membrane; plasma membrane; presynaptic active zone

Molecular Function: G-protein coupled receptor activity; PDZ domain binding; protein binding; Wnt receptor activity; Wnt-protein binding

Biological Process: cell proliferation in midbrain; establishment of planar polarity; G-protein coupled receptor protein signaling pathway; hair follicle development; inner ear morphogenesis; negative regulation of mitotic cell cycle, embryonic; neural tube closure; neuron differentiation; neuron migration; positive regulation of neuroblast proliferation; Wnt receptor signaling pathway through beta-catenin; Wnt receptor signaling pathway, calcium modulating pathway; Wnt receptor signaling pathway, planar cell polarity pathway