Rabbit anti-Human FRG1 Polyclonal Antibody | anti-FRG1 antibody

FRG1, CT (FRG1, Protein FRG1, FSHD region gene 1 protein) (Azide free) (HRP)

Cat. Num. AAA6300107

• Gene Names: FRG1; FSG1; FRG1A
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: FRG1; Polyclonal Antibody; CT (FRG1; Protein FRG1; FSHD region gene 1 protein) (Azide free) (HRP); FSHD region gene 1 protein; anti-FRG1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-FRG1 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: FRG1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 229-257 amino acids from the C-terminal region of human FRG1.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(FRG1 Antibody (C-term) western blot analysis in ZR-75-1 cell line lysates (35ug/lane).This demonstrates the FRG1 antibody detected the FRG1 protein (arrow).)

Related Product Information for anti-FRG1 antibody

This gene maps to a location 100 kb centromeric of the repeat units on chromosome 4q35 which are deleted in facioscapulohumeral muscular dystrophy (FSHD). It is evolutionarily conserved and has related sequences on multiple human chromosomes but DNA sequence analysis did not reveal any homology to known genes. In vivo studies demonstrate the encoded protein is localized to the nucleolus.

Product Categories/Family for anti-FRG1 antibody

Antibodies; Hormones; Steroids

References

Hanel, M.L., et al. Dev. Dyn. 238(6):1502-1512(2009). Bodega, B., et al. BMC Biol. 7, 41 (2009): Pirozhkova, I., et al. PLoS ONE 3 (10), E3389 (2008): Lamesch, P., et al. Genomics 89(3):307-315(2007). Gabellini, D., et al. Nature 439(7079):973-977(2006).

NCBI and Uniprot Product Information

• NCBI GI #: 4758404
• NCBI GeneID: 2483
• NCBI Accession #: NP_004468.1
• NCBI GenBank Nucleotide #: NM_004477.2
• UniProt Accession #: Q14331; A8K775
• Molecular Weight: 29,172 Da
• NCBI Official Full Name: protein FRG1
• NCBI Official Synonym Full Names: FSHD region gene 1
• NCBI Official Symbol: FRG1
• NCBI Official Synonym Symbols: FSG1; FRG1A
• NCBI Protein Information: protein FRG1
• UniProt Protein Name: Protein FRG1
• Protein Family: Protein
• UniProt Gene Name: FRG1
• UniProt Entry Name: FRG1_HUMAN

NCBI Description

This gene maps to a location 100 kb centromeric of the repeat units on chromosome 4q35 which are deleted in facioscapulohumeral muscular dystrophy (FSHD). It is evolutionarily conserved and has related sequences on multiple human chromosomes but DNA sequence analysis did not reveal any homology to known genes. In vivo studies demonstrate the encoded protein is localized to the nucleolus. [provided by RefSeq, Jul 2008]