Western Blot (WB)
(Western blot analysis of fragilis in NIH 3T3 cell lysate in the (A) absence and (B) presence of immunizing peptide using MBS610191 at 1ug/ml.)
Rabbit anti-Mouse Fragilis Polyclonal Antibody | anti-Ifitm3 antibody
Fragilis (1 8U, Ifitm3, Interferon Induced Transmembrane Protein 3, Interferon inducible protein 1 8U, Interferon Inducible Protein 15, Interferon Inducible Protein Homolog)
Gene Names
Ifitm3; Fgls; IP15; Cd225; mil-1; Cdw217; 1110004C05Rik
Reactivity
Mouse
Applications
ELISA, Western Blot
Purity
Affinity PurifiedPurified by immunoaffinity chromatography.
Synonyms
Fragilis; Polyclonal Antibody; Fragilis (1 8U; Ifitm3; Interferon Induced Transmembrane Protein 3; Interferon inducible protein 1 8U; Interferon Inducible Protein 15; Interferon Inducible Protein Homolog); Anti -Fragilis (1 8U; anti-Ifitm3 antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes mouse Fragilis. Species sequence homology: rat.
Purity/Purification
Affinity Purified
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Form/Format
Supplied as a liquid in PBS, 0.2% gelatin, 0.05% sodium azide.
Applicable Applications for anti-Ifitm3 antibody
ELISA (EL/EIA), Western Blot (WB)
Application Notes
Suitable for use in ELISA and Western Blot.
Dilution: Western Blot: 1ug/ml
Dilution: Western Blot: 1ug/ml
Immunogen
Synthetic peptide from amino acid region 1-50 of mouse Fragilis. Cellular Localization: Cell Membrane
Positive Control
NIH 3T3
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Western Blot (WB)
(Western blot analysis of fragilis in NIH 3T3 cell lysate in the (A) absence and (B) presence of immunizing peptide using MBS610191 at 1ug/ml.)
Western Blot (WB)
(Western blot analysis of fragilis in NIH 3T3 cell lysate in the (A) absence and (B) presence of immunizing peptide using MBS610191 at 1ug/ml.)
Related Product Information for anti-Ifitm3 antibody
Interferon-induced transmembrane protein 3(Ifitm3); Fragilis Official Gene Symbol: Ifitm3 Gen Bank Accession Number: NM_025378 Gene ID: 66141(Mouse) Gene Map Locus: 7F5 (Mouse) Fragilis is an IFN-induced transmembrane protein that belongs to the highly conserved fragilis protein family. It consists of an N-terminal signal sequence, 2 putative transmembrane domains, 3 potential phosphorylation sites and C-terminal Leucine-zipper. It is expressed in early embryos and nascent primordial germ cells and is associated with Germ cell specification and development. It is cell surface protein and might be important in mediating interactions amongst germ cells and their surrounding neighbors. It is also suggested that it might be involved in anti-proliferative signaling and homotypic cell adhesion.
Product Categories/Family for anti-Ifitm3 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
14,954 Da
NCBI Official Full Name
fragilis
NCBI Official Synonym Full Names
interferon induced transmembrane protein 3
NCBI Official Symbol
Ifitm3
NCBI Official Synonym Symbols
Fgls; IP15; Cd225; mil-1; Cdw217; 1110004C05Rik
NCBI Protein Information
interferon-induced transmembrane protein 3; fragilis protein; ifitm-like protein 1; interferon-inducible protein 15
UniProt Protein Name
Interferon-induced transmembrane protein 3
Protein Family
UniProt Gene Name
Ifitm3
UniProt Entry Name
IFM3_MOUSE
Uniprot Description
IFITM3: IFN-induced antiviral protein that mediates cellular innate immunity to at least three major human pathogens, namely influenza A H1N1 virus, West Nile virus (WNV), and dengue virus (WNV), by inhibiting the early step(s) of replication. Interacts with SPP1; the interaction reduces OPN expression. By IFN-alpha and IFNG/IFN-gamma. Belongs to the CD225/Dispanin family.
Protein type: Membrane protein, integral; Cell surface
Cellular Component: cell surface; membrane; endoplasmic reticulum; lysosome; apical part of cell; cytoplasmic membrane-bound vesicle; cytoplasm; integral to membrane; plasma membrane; nucleus; endosome
Biological Process: receptor-mediated endocytosis; negative regulation of cell proliferation; negative regulation of viral transcription; negative regulation of viral genome replication; negative regulation of virion penetration into host cell; immune system process; response to virus; response to biotic stimulus; innate immune response; defense response to virus
Research Articles on Ifitm3
Similar Products
Product Notes
The Ifitm3 ifitm3 (Catalog #AAA610191) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Fragilis (1 8U, Ifitm3, Interferon Induced Transmembrane Protein 3, Interferon inducible protein 1 8U, Interferon Inducible Protein 15, Interferon Inducible Protein Homolog) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Fragilis can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB). Suitable for use in ELISA and Western Blot. Dilution: Western Blot: 1ug/ml. Researchers should empirically determine the suitability of the Ifitm3 ifitm3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Fragilis, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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