Rabbit FOSL2 Polyclonal Antibody | anti-FOSL2 antibody

FOSL2 (S301) Polyclonal Antibody

Cat. Num. AAA3000529

• Gene Names: FOSL2; FRA2
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: FOSL2; Polyclonal Antibody; FOSL2 (S301) Polyclonal Antibody; Fos-related antigen 2; FRA-2; FRA2; anti-FOSL2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: FOSL2 (S301) polyclonal antibody detects endogenous levels of FOSL2 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 326

• Applicable Applications for anti-FOSL2 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500-1:1000; IHC: 1:50-1:200
• Immunogen: Synthetic peptide, corresponding to amino acids 270-320 of Human FOSL2.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of FOSL2 (S301) pAb at 1:500 dilutionLane1:The Ovary tissue lysate of Rat (40ug) Lane2:The Testis tissue lysate of Mouse (40ug) Lane3:PC3 whole cell lysate (40ug) Lane4:MCF-7 whole cell lysate (40ug))

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of FOSL2 (S301) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.)

Related Product Information for anti-FOSL2 antibody

The v-Fos oncogene was initially identified as the transforming gene of two independent murine osteosarcoma virus isolates and an avian nephroblastoma virus. The cellular homolog, c-Fos, encodes a nuclear phosphoprotein that is rapidly and transiently induced by a variety of agents and functions as a transcriptional regulator for several genes. In contrast to c-Jun proteins, which form homo- and heterodimers which bind to specific DNA TPA response elements (TREs), c-Fos proteins are only active as heterodimers with members of the Jun gene family. Murine Fos B encodes a nuclear protein of 338 amino acids which has 70% homology with c-Fos, exhibits similar kinetics of expression as c-Fos and forms heterodimers with both c-Jun and Jun B, which bind to TRE DNA response elements. Functional homologs of c-Fos and Fos B include Fra-1 and Fra-2 genes.

NCBI and Uniprot Product Information

• NCBI GI #: 4885245
• NCBI GeneID: 2355
• NCBI Accession #: NP_005244.1
• NCBI GenBank Nucleotide #: NP_005244.1
• UniProt Accession #: P15408
• Molecular Weight: ~ 42kDa
• NCBI Official Full Name: fos-related antigen 2
• NCBI Official Synonym Full Names: FOS like 2, AP-1 transcription factor subunit
• NCBI Official Symbol: FOSL2
• NCBI Official Synonym Symbols: FRA2
• NCBI Protein Information: fos-related antigen 2
• UniProt Protein Name: Fos-related antigen 2
• Protein Family: Fos-related antigen
• UniProt Gene Name: FOSL2
• UniProt Synonym Gene Names: FRA2; FRA-2
• UniProt Entry Name: FOSL2_HUMAN

NCBI Description

The Fos gene family consists of 4 members: FOS, FOSB, FOSL1, and FOSL2. These genes encode leucine zipper proteins that can dimerize with proteins of the JUN family, thereby forming the transcription factor complex AP-1. As such, the FOS proteins have been implicated as regulators of cell proliferation, differentiation, and transformation. [provided by RefSeq, Jul 2014]

Uniprot Description

FRA2: an oncogenic transcription factor of the bZIP family, Fos subfamily. The expression of Fos proteins is rapidly and transiently induced by a variety of extracellular stimuli including growth factors, cytokines, neurotransmitters, polypeptide hormones, and stress. Fos proteins dimerize with Jun proteins (c-Jun, JunB, and JunD) to form Activator Protein-1 (AP-1), a transcription factor that binds to TRE/AP-1 elements and activates transcription. Fos and Jun proteins contain the leucine-zipper motif that mediates dimerization and an adjacent basic domain that binds to DNA. The various Fos/Jun heterodimers differ in their ability to transactivate AP-1 dependent genes. In addition to increased expression, phosphorylation of Fos proteins by Erk kinases in response to extracellular stimuli may further increase transcriptional activity. Following growth factor stimulation, expression of FosB and c-Fos in quiescent fibroblasts is immediate but short-lived, while FRA1 and FRA2 expression persists longer. FRA2 and JUND up-regulate the expression of CCR4, MYB, MDM2, and BCL6, and are expressed at high levels in CCR4-expressing mature T-cell malignancies such as adult T-cell leukemia/lymphoma (ATLL) and cutaneous T-cell lymphomas (CTCLs). May play a role in regulating the transcription of ICOS downstream of TCR/CD28 and cytokine receptor signaling. Its transcription is significantly increased in metastatic clear cell renal cell carcinomas. Belongs to the bZIP family, Fos subfamily. Three isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription factor; DNA-binding

Chromosomal Location of Human Ortholog: 2p23.3

Cellular Component: nucleoplasm; nucleus

Molecular Function: double-stranded DNA binding; transcription factor activity

Biological Process: response to drug; cell death; regulation of transcription from RNA polymerase II promoter; transcription from RNA polymerase II promoter; positive regulation of fibroblast proliferation; response to cAMP; response to mechanical stimulus; response to morphine; female pregnancy; response to progesterone stimulus; cellular response to hormone stimulus; response to corticosterone stimulus