Rabbit anti-Human FPGS Polyclonal Antibody | anti-FPGS antibody

FPGS Antibody

Cat. Num. AAA7110087

• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: >95%, Protein G purified
• Synonyms: FPGS; Polyclonal Antibody; FPGS Antibody; Folylpolyglutamate synthase; mitochondrial; Folylpoly-gamma-glutamate synthetase; Tetrahydrofolylpolyglutamate synthase; Tetrahydrofolate synthase; anti-FPGS antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: >95%, Protein G purified
• Form/Format: Liquid
• Sequence Length: 537

• Applicable Applications for anti-FPGS antibody: ELISA (EIA), Western Blot (WB)
• Application Notes: WB: 1:500-1:5000
• Immunogen: Recombinant Human Folylpolyglutamate synthase, mitochondrial protein (443-546AA)
• Conjugation: Non-conjugated
• Buffer: Preservative: 0.03% Proclin 300; Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
• Preparation and Storage: Upon receipt, store at -20 degree C or -80 degree C. Avoid repeated freeze.

Western Blot (WB)

(Western BlotPositive WB detected in: HepG2 whole cell lysate, MCF-7 whole cell lysateAll lanes: FPGS antibody at 3.2ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 65, 61, 60, 62 KDaObserved band size: 65 KDa)

Related Product Information for anti-FPGS antibody

Catalyzes conversion of folates to polyglutamate derivatives allowing concentration of folate compounds in the cell and the intracellular retention of these cofactors, which are important substrates for most of the folate-dependent enzymes that are involved in one-carbon transfer reactions involved in purine, pyrimidine and amino acid synthesis. Unsubstituted reduced folates are the preferred substrates. Metabolizes methotrexate (MTX) to polyglutamates.

Product Categories/Family for anti-FPGS antibody

Metabolism; Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 66932990
• NCBI GeneID: 2356
• NCBI Accession #: NP_001018088.1
• NCBI GenBank Nucleotide #: NM_001018078.2
• UniProt Accession #: Q05932; Q5JU19; Q5JU22; Q6P2P6; B3KPW4; B7Z2Z3; F5H0K6
• Molecular Weight: 61,562 Da
• NCBI Official Full Name: folylpolyglutamate synthase, mitochondrial isoform b
• NCBI Official Synonym Full Names: folylpolyglutamate synthase
• NCBI Official Symbol: FPGS
• NCBI Protein Information: folylpolyglutamate synthase, mitochondrial
• UniProt Protein Name: Folylpolyglutamate synthase, mitochondrial
• UniProt Gene Name: FPGS
• UniProt Synonym Gene Names: FPGS; Tetrahydrofolate synthase

NCBI Description

This gene encodes the folylpolyglutamate synthetase enzyme. This enzyme has a central role in establishing and maintaining both cytosolic and mitochondrial folylpolyglutamate concentrations and, therefore, is essential for folate homeostasis and the survival of proliferating cells. This enzyme catalyzes the ATP-dependent addition of glutamate moieties to folate and folate derivatives. Alternative splicing results in transcript variants encoding different isoforms. [provided by RefSeq, Jan 2014]

Uniprot Description

Catalyzes conversion of folates to polyglutamate derivatives allowing concentration of folate compounds in the cell and the intracellular retention of these cofactors, which are important substrates for most of the folate-dependent enzymes that are involved in one-carbon transfer reactions involved in purine, pyrimidine and amino acid synthesis. Unsubstituted reduced folates are the preferred substrates. Metabolizes methotrexate (MTX) to polyglutamates.