Rabbit FOXP3 Polyclonal Antibody | anti-FOXP3 antibody

Rabbit anti FOXP3 Polyclonal antibody

Cat. Num. AAA462166

• Gene Names: FOXP3; JM2; AIID; IPEX; PIDX; XPID; DIETER; MGC141961; MGC141963
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: The Rabbit IgG is purified by Epitope Affinity Purification.
• Synonyms: FOXP3; Polyclonal Antibody; Rabbit anti FOXP3 Polyclonal antibody; Rabbit anti-FOXP3; forkhead box P3; JM2; AIID; IPEX; PIDX; XPID; DIETER; SCURFIN; scurfin; JM2 protein; immunodeficiency; polyendocrinopathy; enteropathy; X-linked; immune dysregulation; anti-FOXP3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: This antibody recognizes ~52 kDa of FOXP3 protein. It reacts to human, mouse and rat. The other species are not tested.
• Purity/Purification: The Rabbit IgG is purified by Epitope Affinity Purification.
• Form/Format: This affinity purified antibody is supplied in sterile phosphatebuffered saline (pH7.2) containing antibody stabilizer
• Concentration: Size: 100 ug/200 ul (varies by lot)

• Applicable Applications for anti-FOXP3 antibody: ELISA, Western Blot, Immunohistochemistry
• Application Notes: Western Blot: 0.1-1 ug/ml; ELISA: 0.01-0.1 ug/ml; Immunoprecipitation: 2-5 ug/ml; Immunohistochemistry: 0.5-2 ug/ml
• Antigen Preparation: A synthetic peptide corresponding to the C-terminus of human FOXP3 protein. This sequence is identical within human, mouse, rat origins.
• Positive Control: Hela
• Cellular Location: Nuclear
• Preparation and Storage: The antibodies are stable for 12 months from date of receipt when stored at -20 degree C to -70 degree C. The antibodies can be stored at 2 degree C-8 degree C for three month without detectable loss of activity. Avoid repeated freezing-thawing cycles.

Testing Data

Related Product Information for anti-FOXP3 antibody

Forkhead/winged-helix box (Fox) genes are the transcriptional regulators and highly conserved across mammals. It contains a FOX DNA-binding domain and large polyglutamine tract. The FOXPs are involved in organogenesis and cell differentiation. FOXP3 is essential for normal immune homeostasis. FOXP3 is stably and constitutively expressed at a high level in CD25 + CD4 positive regulatory T cells, at low level in CD4 positive/CD25 negative cells, and is absent in CD4 negative/CD8 positive T cells. FOXP3 may be a master regulatory gene and a more specific marker of regulatory T cells than other T cells.

References

Rao et al. Differentiation and expansion of T cells with regulatory function from human peripheral lymphocytes by stimulation in the presence of TGF-{beta}. J Immunol. 2005 Feb 1;174 (3):1446-55.

NCBI and Uniprot Product Information

• NCBI GI #: 167466190
• NCBI GeneID: 50943
• NCBI Accession #: NP_001107849.1
• NCBI GenBank Nucleotide #: NM_001114377.1
• UniProt Accession #: Q9BZS1; O60827; Q14DD8; Q4ZH51; A5HJT1; B7ZLG0
• Molecular Weight: 52 kDa
• NCBI Official Full Name: forkhead box protein P3 isoform b
• NCBI Official Synonym Full Names: forkhead box P3
• NCBI Official Symbol: FOXP3
• NCBI Official Synonym Symbols: JM2; AIID; IPEX; PIDX; XPID; DIETER; MGC141961; MGC141963
• NCBI Protein Information: forkhead box protein P3; scurfin; FOXP3delta7; OTTHUMP00000025832; OTTHUMP00000025833; OTTHUMP00000226737; immunodeficiency, polyendocrinopathy, enteropathy, X-linked; immune dysregulation, polyendocrinopathy, enteropathy, X-linked
• UniProt Protein Name: Forkhead box protein P3
• Protein Family: Forkhead box protein
• UniProt Gene Name: FOXP3
• UniProt Synonym Gene Names: IPEX
• UniProt Entry Name: FOXP3_HUMAN

NCBI Description

The protein encoded by this gene is a member of the forkhead/winged-helix family of transcriptional regulators. Defects in this gene are the cause of immunodeficiency polyendocrinopathy, enteropathy, X-linked syndrome (IPEX), also known as X-linked autoimmunity-immunodeficiency syndrome. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq]

Uniprot Description

Function: Probable transcription factor. Plays a critical role in the control of immune response.

Subunit structure: Interacts with IKZF3. Ref.7

Subcellular location: Nucleus

Potential.

Involvement in disease: Defects in FOXP3 are the cause of immunodeficiency polyendocrinopathy, enteropathy, X-linked syndrome (IPEX) [

MIM:304790]; also known as X-linked autoimmunity-immunodeficiency syndrome. IPEX is characterized by neonatal onset insulin-dependent diabetes mellitus, infections, secretory diarrhea, trombocytopenia, anemia and eczema. It is usually lethal in infancy. Ref.8 Ref.9 Ref.10 Ref.11

Sequence similarities: Contains 1 C2H2-type zinc finger.Contains 1 fork-head DNA-binding domain.