Mouse G Protein Coupled Receptor 120 (GPR120) ELISA Kit | GPR120 elisa kit

Mouse G Protein Coupled Receptor 120 (GPR120) ELISA Kit

Cat. Num. AAA2019916

• Gene Names: FFAR4; GT01; PGR4; BMIQ10; GPR120; GPR129; O3FAR1
• Reactivity: Mouse
• Synonyms: G Protein Coupled Receptor 120 (GPR120); Mouse G Protein Coupled Receptor 120 (GPR120) ELISA Kit; GPR129; GT01; PGR4; GPR120; O3FAR1; G Protein Coupled Receptor 120; Omega-3 fatty acid receptor 1; GPR120 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Free Fatty Acid Receptor 4 (FFAR4).; No significant cross-reactivity or interference between Free Fatty Acid Receptor 4 (FFAR4) and analogues was observed.
• Sequence Length: 376

• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates, Cell lysates and Other Biological Fluids
• Detection Range: 0.312-20ng/mL
• Sensitivity: < 0.123ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Free Fatty Acid Receptor 4 (FFAR4) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Free Fatty Acid Receptor 4 (FFAR4) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for GPR120 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Free Fatty Acid Receptor 4 (FFAR4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Free Fatty Acid Receptor 4 (FFAR4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Free Fatty Acid Receptor 4 (FFAR4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Free Fatty Acid Receptor 4 (FFAR4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for GPR120 elisa kit

CD & Adhesion molecule

NCBI and Uniprot Product Information

• NCBI GI #: 32165518
• NCBI GeneID: 338557
• UniProt Accession #: Q5NUL3; Q495H1; Q5VY25; Q5VY26; Q7Z605; Q86SM7
• Molecular Weight: 40,494 Da
• NCBI Official Full Name: G protein-coupled receptor 120
• NCBI Official Synonym Full Names: free fatty acid receptor 4
• NCBI Official Symbol: FFAR4
• NCBI Official Synonym Symbols: GT01; PGR4; BMIQ10; GPR120; GPR129; O3FAR1
• NCBI Protein Information: free fatty acid receptor 4
• UniProt Protein Name: Free fatty acid receptor 4
• UniProt Gene Name: FFAR4
• UniProt Synonym Gene Names: GPR120; GPR129; O3FAR1; PGR4
• UniProt Entry Name: FFAR4_HUMAN

NCBI Description

This gene encodes a G protein-coupled receptor (GPR) which belongs to the rhodopsin family of GPRs. The encoded protein functions as a receptor for free fatty acids, including omega-3, and participates in suppressing anti-inflammatory responses and insulin sensitizing. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2012]

Uniprot Description

GPR120: Receptor for medium and long-chain free fatty acid (FAA). Signals via a G(q)/G(11)-coupled pathway. Acts as a receptor for omega-3 fatty acids and mediates robust anti- inflammatory effects particularly in macrophages and fat cells. The anti-inflammatory effects involve inhibition of TAK1 through a beta-arrestin 2 (ARRB2)/TAB1 dependent effect but independent of G(q)/G(11)-coupled pathway. Mediates potent insulin sensitizing and antidiabetic effects by repressing macrophage-induced tissue inflammation. May mediate the taste of fatty acids. Belongs to the G-protein coupled receptor 1 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Membrane protein, multi-pass; Lipid-binding; GPCR, family 1; Receptor, GPCR

Chromosomal Location of Human Ortholog: 10q23.33

Cellular Component: integral to plasma membrane; endocytic vesicle; plasma membrane

Molecular Function: fatty acid binding; peptide binding; taste receptor activity

Biological Process: hormone secretion; G-protein coupled receptor protein signaling pathway; negative regulation of inflammatory response; negative regulation of cytokine secretion; detection of chemical stimulus involved in sensory perception of taste; negative regulation of apoptosis; cellular response to hormone stimulus

Disease: Body Mass Index Quantitative Trait Locus 10