Rabbit FoxO4 Polyclonal Antibody | anti-FoxO4 antibody

FoxO4 (Phospho-S197) Polyclonal Antibody

Cat. Num. AAA3005210

• Gene Names: FOXO4; AFX; AFX1; MLLT7
• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: FoxO4; Polyclonal Antibody; FoxO4 (Phospho-S197) Polyclonal Antibody; Forkhead box protein O4; Fork head domain transcription factor AFX1; AFX; AFX1; MLLT7; FOXO4; anti-FoxO4 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: p-FoxO4 (S197) polyclonal antibody detects endogenous levels of FoxO4 protein only when phosphorylated at Ser197.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 450

• Applicable Applications for anti-FoxO4 antibody: Immunohistochemistry (IHC)
• Application Notes: IHC: 1:50-1:200
• Immunogen: Synthetic phosphopeptide derived from human FoxO4 around the phosphorylation site of Serine 197.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of p-FOXO4 (S197) pAb in paraffin-embedded human brain tissue.)

Related Product Information for anti-FoxO4 antibody

FOX4 is a forkhead transcription factor involved in the regulation of the insulin signaling pathway. It binds to insulin-response elements (IREs) and can activate transcription of IGFBP1. FOXO4 down-regulates expression of HIF1A and suppresses hypoxia-induced transcriptional activation of HIF1A-modulated genes. It is also involved in negative regulation of the cell cycle.

NCBI and Uniprot Product Information

• NCBI GI #: 283436083
• NCBI GeneID: 4303
• NCBI Accession #: NP_001164402.1
• NCBI GenBank Nucleotide #: NP_001164402.1
• UniProt Accession #: P98177
• Molecular Weight: ~ 55, 70kDa
• NCBI Official Full Name: forkhead box protein O4 isoform 2
• NCBI Official Synonym Full Names: forkhead box O4
• NCBI Official Symbol: FOXO4
• NCBI Official Synonym Symbols: AFX; AFX1; MLLT7
• NCBI Protein Information: forkhead box protein O4
• UniProt Protein Name: Forkhead box protein O4
• Protein Family: Forkhead box protein
• UniProt Gene Name: FOXO4
• UniProt Synonym Gene Names: AFX; AFX1; MLLT7
• UniProt Entry Name: FOXO4_HUMAN

NCBI Description

This gene encodes a member of the O class of winged helix/forkhead transcription factor family. Proteins encoded by this class are regulated by factors involved in growth and differentiation indicating they play a role in these processes. A translocation involving this gene on chromosome X and the homolog of the Drosophila trithorax gene, encoding a DNA binding protein, located on chromosome 11 is associated with leukemia. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2010]

Uniprot Description

FOXO4: transcription factor AFX1 containing 1 fork-head domain. May play a role in the insulin signaling pathway. Involved in acute leukemias by a chromosomal translocation t(X;11)(q13;q23) that involves MLLT7 and MLL/HRX. The result is a rogue activator protein. Two splice-variant isoforms have been described.

Protein type: DNA-binding; Oncoprotein; Transcription factor

Chromosomal Location of Human Ortholog: Xq13.1

Cellular Component: nucleoplasm; cytoplasm; cytosol; nucleus

Molecular Function: protein binding; enzyme binding; DNA binding; sequence-specific DNA binding; transcription factor activity; transcription factor binding

Biological Process: epidermal growth factor receptor signaling pathway; transcription from RNA polymerase II promoter; muscle development; phosphoinositide-mediated signaling; fibroblast growth factor receptor signaling pathway; nerve growth factor receptor signaling pathway; positive regulation of transcription, DNA-dependent; stem cell differentiation; negative regulation of smooth muscle cell differentiation; regulation of transcription from RNA polymerase II promoter; negative regulation of cell proliferation; negative regulation of angiogenesis; regulation of transcription, DNA-dependent; mitotic cell cycle G2/M transition DNA damage checkpoint; insulin receptor signaling pathway; innate immune response; cell cycle arrest