Rabbit FOXO4 Polyclonal Antibody | anti-FOXO4 antibody

FOXO4 Antibody

Cat. Num. AAA150053

• Gene Names: FOXO4; AFX; AFX1; MLLT7
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunocytochemistry, Immunofluorescence
• Purity: FOXO4 Antibody is affinity chromatography purified via peptide column.
• Synonyms: FOXO4; Polyclonal Antibody; FOXO4 Antibody; AFX; AFX1; MLLT7; Forkhead box protein O4; Fork head domain transcription factor AFX1; forkhead box O4; anti-FOXO4 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: FOXO4 Antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 505

• Applicable Applications for anti-FOXO4 antibody: ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)
• Application Notes: FOXO4 antibody can be used for detection of FOXO4 by Western blot at 0.5 - 1 mug/mL. Antibody can also be used for immunocytochemistry starting at 10 mug/mL. For immunofluorescence start at 20 mug/mL.
• Conjugate: Unconjugated
• Immunogen: FOXO4 antibody was raised against a 16 amino acid synthetic peptide near the amino terminus of human FOXO4.
• Buffer: FOXO4 Antibody is supplied in PBS containing 0.02% sodium azide.
• Preparation and Storage: FOXO4 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of FOXO4 in HeLa cell lysate with FOXO4 antibody at (A) 0.5 and (B) 1 μg/mL.)

Immunocytochemistry (IHC)

(Immunocytochemistry of FOXO4 in HeLa cells with FOXO4 antibody at 10 μg/mL.)

Related Product Information for anti-FOXO4 antibody

FOXO4 Antibody: FOXO4 is a ubiquitously expressed protein member of a subfamily of the forkhead homeotic gene family of transcription factors and shuttles between the cytoplasm and nucleus. FOXO transcription factors are key players of cell fate decisions, metabolism, stress resistance, tumor suppression and are regulated by growth factors, oxidative stress or nutrient deprivation. In the absence of PI3K/AKT activation, FOXO4 localizes in the nucleus where it functions as a transcription factor. FOXO4 can also be phosphorylated by JNK following induction of reactive oxygen species (ROS), resulting in transcriptional activation and the induction of a negative feedback mechanism to counteract the ROS. It is through this mechanism that FOXO4 is thought to sensitize cancer cells to doxorubicin-mediated toxicity.

NCBI and Uniprot Product Information

• NCBI GI #: 103472003
• NCBI GeneID: 4303
• NCBI Accession #: NP_005929
• NCBI GenBank Nucleotide #: NM_005938.3
• UniProt Accession #: P98177; O43821; Q13720; Q3KPF1; Q8TDK9; B7WPJ7
• Molecular Weight: 47,941 Da
• NCBI Official Full Name: forkhead box protein O4 isoform 1
• NCBI Official Synonym Full Names: forkhead box O4
• NCBI Official Symbol: FOXO4
• NCBI Official Synonym Symbols: AFX; AFX1; MLLT7
• NCBI Protein Information: forkhead box protein O4; fork head domain transcription factor AFX1; myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila); translocated to, 7
• UniProt Protein Name: Forkhead box protein O4
• Protein Family: Forkhead box protein
• UniProt Gene Name: FOXO4
• UniProt Synonym Gene Names: AFX; AFX1; MLLT7
• UniProt Entry Name: FOXO4_HUMAN

NCBI Description

This gene encodes a member of the O class of winged helix/forkhead transcription factor family. Proteins encoded by this class are regulated by factors involved in growth and differentiation indicating they play a role in these processes. A translocation involving this gene on chromosome X and the homolog of the Drosophila trithorax gene, encoding a DNA binding protein, located on chromosome 11 is associated with leukemia. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2010]

Uniprot Description

FOXO4: transcription factor AFX1 containing 1 fork-head domain. May play a role in the insulin signaling pathway. Involved in acute leukemias by a chromosomal translocation t(X;11)(q13;q23) that involves MLLT7 and MLL/HRX. The result is a rogue activator protein. Two splice-variant isoforms have been described.

Protein type: Oncoprotein; DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: Xq13.1

Cellular Component: nucleoplasm; cytoplasm; cytosol; nucleus

Molecular Function: protein binding; enzyme binding; DNA binding; sequence-specific DNA binding; transcription factor activity; transcription factor binding

Biological Process: transcription from RNA polymerase II promoter; epidermal growth factor receptor signaling pathway; muscle development; phosphoinositide-mediated signaling; fibroblast growth factor receptor signaling pathway; nerve growth factor receptor signaling pathway; positive regulation of transcription, DNA-dependent; stem cell differentiation; negative regulation of smooth muscle cell differentiation; regulation of transcription from RNA polymerase II promoter; negative regulation of cell proliferation; negative regulation of angiogenesis; regulation of transcription, DNA-dependent; mitotic cell cycle G2/M transition DNA damage checkpoint; insulin receptor signaling pathway; innate immune response; cell cycle arrest