Human Forkhead Box Protein P1 (FOXP1) ELISA Kit | FOXP1 elisa kit

Human Forkhead Box Protein P1 (FOXP1) ELISA Kit

Cat. Num. AAA2088143

• Gene Names: FOXP1; MFH; QRF1; 12CC4; hFKH1B; HSPC215
• Reactivity: Human
• Synonyms: Forkhead Box Protein P1 (FOXP1); Human Forkhead Box Protein P1 (FOXP1) ELISA Kit; FOXP1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Forkhead Box Protein P1 (FOXP1).; No significant cross-reactivity or interference between Forkhead Box Protein P1 (FOXP1) and analogues was observed.
• Sequence Length: 114

• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates, Cell lysates, Cell culture supernates and Other biological fluid
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.055ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Forkhead Box Protein P1 (FOXP1) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Forkhead Box Protein P1 (FOXP1) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for FOXP1 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Forkhead Box Protein P1 (FOXP1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Forkhead Box Protein P1 (FOXP1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Forkhead Box Protein P1 (FOXP1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Forkhead Box Protein P1 (FOXP1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for FOXP1 elisa kit

Apoptosis; Tumor immunity; Immune molecule; Neuroscience; Developmental science; Pulmonology; Autoimmunity

NCBI and Uniprot Product Information

• NCBI GI #: 60498987
• NCBI GeneID: 27086
• NCBI Accession #: NP_001012523.1
• NCBI GenBank Nucleotide #: NM_001012505.1
• UniProt Accession #: Q9H334; Q8NAN6; Q9BSG9; Q9H332; Q9H333; Q9P0R1; A3QVP8; B3KV70; G5E9V8
• Molecular Weight: 77,249 Da
• NCBI Official Full Name: forkhead box protein P1 isoform 2
• NCBI Official Synonym Full Names: forkhead box P1
• NCBI Official Symbol: FOXP1
• NCBI Official Synonym Symbols: MFH; QRF1; 12CC4; hFKH1B; HSPC215
• NCBI Protein Information: forkhead box protein P1
• UniProt Protein Name: Forkhead box protein P1
• Protein Family: Forkhead box protein
• UniProt Gene Name: FOXP1
• UniProt Synonym Gene Names: MFH

NCBI Description

This gene belongs to subfamily P of the forkhead box (FOX) transcription factor family. Forkhead box transcription factors play important roles in the regulation of tissue- and cell type-specific gene transcription during both development and adulthood. Forkhead box P1 protein contains both DNA-binding- and protein-protein binding-domains. This gene may act as a tumor suppressor as it is lost in several tumor types and maps to a chromosomal region (3p14.1) reported to contain a tumor suppressor gene(s). Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

Uniprot Description

FOXP1: Transcriptional repressor. It plays an important role in the specification and differentiation of lung epithelium. Can act with CTBP1 to synergistically repress transcription but CTPBP1 is not essential. Essential transcriptional regulator of B-cell development. Forms homodimers and heterodimers with FOXP2 and FOXP4. Dimerization is required for DNA-binding. Interacts with CTBP1. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: C2H2-type zinc finger protein; Cell cycle regulation; DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 3p13

Cellular Component: nucleoplasm; nucleus

Molecular Function: androgen receptor binding; protein binding; protein self-association

Biological Process: endothelial cell activation; macrophage activation; monocyte activation; negative regulation of B cell apoptosis; negative regulation of transcription, DNA-dependent; osteoclast differentiation; positive regulation of smooth muscle cell proliferation; regulation of inflammatory response; regulation of interleukin-1 beta secretion; regulation of monocyte differentiation; regulation of tumor necrosis factor production; response to lipopolysaccharide

Disease: Mental Retardation With Language Impairment And With Or Without Autistic Features