Rabbit FOXO4 / AFX1 Polyclonal Antibody | anti-FOXO4 antibody

Rabbit Polyclonal to Human FOXO4 / AFX1

Cat. Num. AAA247252

• Gene Names: FOXO4; AFX; AFX1; MLLT7
• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry, Immunocytochemistry, Western Blot, ELISA
• Purity: Immunoaffinity Purified
• Synonyms: FOXO4 / AFX1; Polyclonal Antibody; Rabbit Polyclonal to Human FOXO4 / AFX1; Anti-FOXO4 / AFX1 Antibody (N-Terminus) IHC-plus; FOXO4; AFX; AFX1; Forkhead box O4; Forkhead box protein O4; MLLT7; Human FOXO4; anti-FOXO4 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: Human FOXO4
• Purity/Purification: Immunoaffinity Purified
• Form/Format: PBS, 0.02% sodium azide
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 505

• Applicable Applications for anti-FOXO4 antibody: Immunohistochemistry (IHC - Paraffin), Immunocytochemistry (ICC), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA, ICC, IHC-P (5 ug/ml), WB (0.5 - 1 ug/ml); Usage: FOXO4 antibody can be used for detection of FOXO4 by Western blot at 0.5-1 ug/ml.
• Target Species: Human
• Immunogen Description: A 16 amino acid peptide near the amino terminus of human FOXO4.
• Immunogen Type: Synthetic peptide
• Immunogen: FOXO4 / AFX1 antibody was raised against a 16 amino acid peptide near the amino terminus of human FOXO4.
• Antigen Modification: N-Terminus
• Preparation and Storage: Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles. Store undiluted.

Immunohistochemistry (IHC)

(Anti-FOXO4 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Immunohistochemistry (IHC)

(Anti-FOXO4 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Immunohistochemistry (IHC)

(Anti-FOXO4 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Immunocytochemistry (ICC)

(Immunocytochemistry of FOXO4 in HeLa cells with FOXO4 antibody at 10 ug/ml.)

Western Blot (WB)

(Western blot analysis of FOXO4 in HeLa cell lysate with FOXO4 antibody at (A) 0.5 and (B) 1 ug/ml.)

Product Categories/Family for anti-FOXO4 antibody

Family: Transcription factor

NCBI and Uniprot Product Information

• NCBI GI #: 103472003
• NCBI GeneID: 4303
• NCBI Accession #: NP_005929.2
• NCBI GenBank Nucleotide #: NM_005938.3
• UniProt Accession #: P98177; O43821; Q13720; Q3KPF1; Q8TDK9; B7WPJ7
• Molecular Weight: 47,941 Da
• NCBI Official Full Name: forkhead box protein O4 isoform 1
• NCBI Official Synonym Full Names: forkhead box O4
• NCBI Official Symbol: FOXO4
• NCBI Official Synonym Symbols: AFX; AFX1; MLLT7
• NCBI Protein Information: forkhead box protein O4; fork head domain transcription factor AFX1; myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila); translocated to, 7
• UniProt Protein Name: Forkhead box protein O4
• Protein Family: Forkhead box protein
• UniProt Gene Name: FOXO4
• UniProt Synonym Gene Names: AFX; AFX1; MLLT7
• UniProt Entry Name: FOXO4_HUMAN

NCBI Description

This gene encodes a member of the O class of winged helix/forkhead transcription factor family. Proteins encoded by this class are regulated by factors involved in growth and differentiation indicating they play a role in these processes. A translocation involving this gene on chromosome X and the homolog of the Drosophila trithorax gene, encoding a DNA binding protein, located on chromosome 11 is associated with leukemia. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2010]

Uniprot Description

FOXO4: transcription factor AFX1 containing 1 fork-head domain. May play a role in the insulin signaling pathway. Involved in acute leukemias by a chromosomal translocation t(X;11)(q13;q23) that involves MLLT7 and MLL/HRX. The result is a rogue activator protein. Two splice-variant isoforms have been described.

Protein type: Transcription factor; Oncoprotein; DNA-binding

Chromosomal Location of Human Ortholog: Xq13.1

Cellular Component: nucleoplasm; cytoplasm; cytosol; nucleus

Molecular Function: protein binding; enzyme binding; DNA binding; sequence-specific DNA binding; transcription factor binding; transcription factor activity

Biological Process: epidermal growth factor receptor signaling pathway; transcription from RNA polymerase II promoter; muscle development; fibroblast growth factor receptor signaling pathway; phosphoinositide-mediated signaling; nerve growth factor receptor signaling pathway; positive regulation of transcription, DNA-dependent; negative regulation of smooth muscle cell differentiation; stem cell differentiation; regulation of transcription from RNA polymerase II promoter; negative regulation of cell proliferation; negative regulation of angiogenesis; regulation of transcription, DNA-dependent; mitotic cell cycle G2/M transition DNA damage checkpoint; insulin receptor signaling pathway; innate immune response; cell cycle arrest