Rabbit FOXO3A Polyclonal Antibody | anti-FOXO3A antibody

RABBIT ANTI FOXO3A

Cat. Num. AAA223583

• Gene Names: FOXO3; FOXO2; AF6q21; FKHRL1; FOXO3A; FKHRL1P2
• Reactivity: Bovine (Expected from Sequence), Chicken (Expected from Sequence), Dog (Expected from Sequence), Monkey (Expected from Sequence), Mouse (Expected from Sequence), Rat (Expected from Sequence), Xenopus (Expected from Sequence)
• Applications: Immunohistochemistry, Western Blot
• Synonyms: FOXO3A; Polyclonal Antibody; RABBIT ANTI FOXO3A; anti-FOXO3A antibody

• Host: Rabbit
• Reactivity: Bovine (Expected from Sequence), Chicken (Expected from Sequence), Dog (Expected from Sequence), Monkey (Expected from Sequence), Mouse (Expected from Sequence), Rat (Expected from Sequence), Xenopus (Expected from Sequence)
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: This item recognises human forkhead box O3A (FOXO3A), also known as FKHLR1 and FOXO3, a 95kDa member of the forkhead family of transcription factors. FOXO3A is ubiquitously expressed and is an important regulator of apoptosis and the cell cycle. FOXO3A triggers apoptosis by inducing the expression of genes necessary for cell death.
• Form/Format: Purified; Purified IgG - liquid
• Concentration: Total protein concentration 0.5mg/ml (varies by lot)
• Sequence Length: 673

• Applicable Applications for anti-FOXO3A antibody: Immunohistology Paraffin, Western Blot (WB)
• Application Notes: Immunohistology: This product does not require protein digestion pre-treatment of paraffin sections. This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections. ; Immunohistology - Paraffin: Minimum Dilution: 10ug/ml; Western Blotting: Minimum Dilution: 2; Maximum Dilution: 4ug/ml
• Perservative Stabilisers: 0.05% Sodium Azide (NaN3); 0.5% Bovine Serum Albumin
• Immunogen: Synthetic peptide corresponding to a portion of the amino acid sequence between 600-673 of human FOXO3A - KLH conjugated.; Histology Positive Control Tissue
• Antiserum Preparation: Antisera to FOXO3A were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG prepared from whole serum by affinity chromatography.
• Target Species: Human
• Preparation and Storage: Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 18 months from date of despatch.

Testing Data #1

(Staining of formalin fixed, paraffin embedded human skin with Rabbit anti Human FOXO3A (MBS223583))

Related Product Information for anti-FOXO3A antibody

MBS223583 recognises human forkhead box O3A (FOXO3A), also known as FKHLR1 and FOXO3, a 95kDa member of the forkhead family of transcription factors. FOXO3A is ubiquitously expressed and is an important regulator of apoptosis and the cell cycle. FOXO3A triggers apoptosis by inducing the expression of genes necessary for cell death.

NCBI and Uniprot Product Information

• NCBI GI #: 4503739
• NCBI GeneID: 2309
• NCBI Accession #: NP_001446.1
• NCBI GenBank Nucleotide #: NM_001455.3
• UniProt Accession #: O43524; O15171; Q5T2I7; Q9BZ04; B4DVZ6; E1P5E6
• Molecular Weight: 48,406 Da
• NCBI Official Full Name: forkhead box protein O3
• NCBI Official Synonym Full Names: forkhead box O3
• NCBI Official Symbol: FOXO3
• NCBI Official Synonym Symbols: FOXO2; AF6q21; FKHRL1; FOXO3A; FKHRL1P2
• NCBI Protein Information: forkhead box protein O3; forkhead box O3A; forkhead homolog (rhabdomyosarcoma) like 1; forkhead in rhabdomyosarcoma-like 1; forkhead, Drosophila, homolog of, in rhabdomyosarcoma-like 1
• UniProt Protein Name: Forkhead box protein O3
• UniProt Gene Name: FOXO3
• UniProt Synonym Gene Names: FKHRL1; FOXO3A
• UniProt Entry Name: FOXO3_HUMAN

NCBI Description

This gene belongs to the forkhead family of transcription factors which are characterized by a distinct forkhead domain. This gene likely functions as a trigger for apoptosis through expression of genes necessary for cell death. Translocation of this gene with the MLL gene is associated with secondary acute leukemia. Alternatively spliced transcript variants encoding the same protein have been observed. [provided by RefSeq, Jul 2008]

Uniprot Description

FOXO3A: a transcription factors of the forkhead family. Contains 1 fork-head domain. May regulate apoptosis through control of genes necessary for cell death. Translocation of this gene with the MLL gene is associated with secondary acute leukemia.

Protein type: Oncoprotein; Transcription factor; DNA-binding; Autophagy

Chromosomal Location of Human Ortholog: 6q21

Cellular Component: nucleoplasm; membrane; cytoplasm; cytosol; nucleus

Molecular Function: protein binding; DNA binding; sequence-specific DNA binding; chromatin DNA binding; transcription factor activity; protein kinase binding

Biological Process: transcription from RNA polymerase II promoter; epidermal growth factor receptor signaling pathway; phosphoinositide-mediated signaling; fibroblast growth factor receptor signaling pathway; nerve growth factor receptor signaling pathway; DNA damage response, signal transduction by p53 class mediator; initiation of primordial ovarian follicle growth; positive regulation of erythrocyte differentiation; positive regulation of transcription, DNA-dependent; antral ovarian follicle growth; negative regulation of transcription from RNA polymerase II promoter; glucose homeostasis; ovulation from ovarian follicle; oocyte maturation; regulation of translation; regulation of transcription from RNA polymerase II promoter; positive regulation of neuron apoptosis; innate immune response; positive regulation of transcription from RNA polymerase II promoter