Rabbit anti-Human, Mouse FOXN1 Polyclonal Antibody | anti-FOXN1 antibody

FOXN1 Antibody

Cat. Num. AAA7048189

• Gene Names: FOXN1; WHN; RONU; FKHL20
• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: >95%, Protein G purified
• Synonyms: FOXN1; Polyclonal Antibody; FOXN1 Antibody; Forkhead box protein N1; Winged-helix transcription factor nude; RONU; WHN; anti-FOXN1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: >95%, Protein G purified
• Form/Format: Liquid
• Sequence Length: 648

• Applicable Applications for anti-FOXN1 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500-1:5000, IHC: 1:20-1:200
• Immunogen: Recombinant Human Forkhead box protein N1 protein (1-160AA)
• Storage Buffer: Preservative: 0.03% Proclin 300; Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
• Conjugation: Non-conjugated
• Santa Cruz Alternative: Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-23566 / sc-26330 / sc-26331 / sc-30195
• Species: Human
• Research Area: Immunology
• Target Names: FOXN1
• Preparation and Storage: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Western Blot (WB)

(Western BlotPositive WB detected in: Raji whole cell lysate,Jurkat whole cell lysate,NIH/3T3 whole cell lysate,Mouse lung tissue,Mouse stomach tissue,Rat thymus tissueAll lanes: FOXN1 antibody at 2.7ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 69 KDaObserved band size: 69 KDa)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human thyroid tissue using MBS7048189 at dilution of 1:100)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human cervical cancer using MBS7048189 at dilution of 1:100)

Related Product Information for anti-FOXN1 antibody

Transcriptional regulator which regulates the development, differentiation, and function of thymic epithelial cells (TECs) both in the prenatal and postnatal thymus. Acts as a master regulator of the TECs lineage development and is required from the onset of differentiation in progenitor TECs in the developing fetus to the final differentiation steps through which TECs mature to acquire their full functionality. Regulates, either directly or indirectly the expression of a variety of genes that mediate diverse aspects of thymus development and function, including MHC Class II, DLL4, CCL25, CTSL, CD40 and PAX1. Regulates the differentiation of the immature TECs into functional cortical TECs (cTECs) and medullary TECs (mTECs). Essential for maintenance of mTECs population in the postnatal thymus. Involved in the morphogenesis and maintenance of the three-dimensional thymic microstructure which is necessary for a fully functional thymus. Plays an important role in the maintenance of hematopoiesis and particularly T lineage progenitors within the bone marrow niche with age. Essential for the vascularization of the thymus anlage. Promotes the terminal differentiation of epithelial cells in the epidermis and hair follicles, partly by negatively regulating the activity of protein kinase C (By similarity). Plays a crucial role in the early prenatal stages of T-cell ontogeny (PubMed: 21507891).

NCBI and Uniprot Product Information

• NCBI GI #: 18201913
• NCBI GeneID: 8456
• NCBI Accession #: NP_003584.2
• NCBI GenBank Nucleotide #: NM_003593.2
• UniProt Accession #: O15353; O15352; B2R9Q7
• NCBI Official Full Name: forkhead box protein N1
• NCBI Official Synonym Full Names: forkhead box N1
• NCBI Official Symbol: FOXN1
• NCBI Official Synonym Symbols: WHN; RONU; FKHL20
• NCBI Protein Information: forkhead box protein N1
• UniProt Protein Name: Forkhead box protein N1
• Protein Family: Forkhead box protein
• UniProt Gene Name: FOXN1
• UniProt Synonym Gene Names: RONU; WHN
• UniProt Entry Name: FOXN1_HUMAN

NCBI Description

Mutations in the winged-helix transcription factor gene at the nude locus in mice and rats produce the pleiotropic phenotype of hairlessness and athymia, resulting in a severely compromised immune system. This gene is orthologous to the mouse and rat genes and encodes a similar DNA-binding transcription factor that is thought to regulate keratin gene expression. A mutation in this gene has been correlated with T-cell immunodeficiency, the skin disorder congenital alopecia, and nail dystrophy. Alternative splicing in the 5' UTR of this gene has been observed. [provided by RefSeq, Jul 2008]

Uniprot Description

FOXN1: Transcriptional regulator involved in development. Defects in FOXN1 are the cause of T-cell immunodeficiency congenital alopecia and nail dystrophy (TIDAND). A disorder characterized by the association of congenital alopecia, severe T-cell immunodeficiency, and ridging and pitting of all nails.

Protein type: Cell development/differentiation; DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 17q11.2

Biological Process: defense response; epidermis development; organ morphogenesis; positive regulation of epithelial cell differentiation; regulation of transcription from RNA polymerase II promoter; transcription from RNA polymerase II promoter

Disease: T-cell Immunodeficiency, Congenital Alopecia, And Nail Dystrophy