Rabbit anti-Human FOXL2 Polyclonal Antibody | anti-FOXL2 antibody

FOXL2 Antibody (N-term)

Cat. Num. AAA9204942

• Gene Names: FOXL2; BPES; PFRK; POF3; BPES1; PINTO
• Reactivity: Human
• Applications: Western Blot, ELISA, Flow Cytometry, Functional Assay
• Purity: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: FOXL2; Polyclonal Antibody; FOXL2 Antibody (N-term); Forkhead box protein L2; anti-FOXL2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This FOXL2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 3-29 amino acids from the N-terminal region of human FOXL2.
• Purity/Purification: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Concentration: Vial Concentration: 0.5 (varies by lot)
• Sequence Positions: 3-29
• Sequence Length: 376

• Applicable Applications for anti-FOXL2 antibody: Western Blot (WB), ELISA (EIA), Flow Cytometry (FC/FACS)
• Application Notes: WB~~1:1000
• Antigen Type: Synthetic Peptide
• Antigen Source: HUMAN
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(FOXL2 Antibody (N-term) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the FOXL2 antibody detected the FOXL2 protein (arrow).)

Flow Cytometry (FC/FACS)

(FOXL2 Antibody (N-term) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-FOXL2 antibody

This gene encodes a forkhead transcription factor. The protein contains a fork-head DNA-binding domain and may play a role in ovarian development and function. Mutations in this gene are a cause of blepharophimosis syndrome and premature ovarian failure 3.

References

Nakashima, M., et al. Nat. Genet. 42(9):768-771(2010) Kraoua, L., et al. Clin. Genet. 77(6):601-603(2010) Murphy, B.D. Nat. Med. 16(5):527-529(2010) Corpuz, P.S., et al. Mol. Endocrinol. 24(5):1037-1051(2010) Kim, T., et al. Histopathology 56(3):408-410(2010)

NCBI and Uniprot Product Information

• NCBI GI #: 12751477
• NCBI GeneID: 668
• NCBI Accession #: NP_075555.1
• NCBI GenBank Nucleotide #: NM_023067.3
• UniProt Accession #: P58012; Q4ZGJ3
• Molecular Weight: 38772
• NCBI Official Full Name: forkhead box protein L2
• NCBI Official Synonym Full Names: forkhead box L2
• NCBI Official Symbol: FOXL2
• NCBI Official Synonym Symbols: BPES; PFRK; POF3; BPES1; PINTO
• NCBI Protein Information: forkhead box protein L2
• UniProt Protein Name: Forkhead box protein L2
• Protein Family: Forkhead box protein
• UniProt Gene Name: FOXL2
• UniProt Entry Name: FOXL2_HUMAN

NCBI Description

This gene encodes a forkhead transcription factor. The protein contains a fork-head DNA-binding domain and may play a role in ovarian development and function. Mutations in this gene are a cause of blepharophimosis syndrome and premature ovarian failure 3. [provided by RefSeq, Jun 2009]

Uniprot Description

FOXL2: Transcriptional regulator. Critical factor essential for ovary differentiation and maintenance, and repression of the genetic program for somatic testis determination. Prevents trans- differentiation of ovary to testis through transcriptional repression of the Sertoli cell-promoting gene SOX9. Has apoptotic activity in ovarian cells. Suppresses ESR1-mediated transcription of PTGS2/COX2 stimulated by tamoxifen. Is a regulator of CYP19 expression. Participates in SMAD3-dependent transcription of FST via the intronic SMAD-binding element. Is a transcriptional repressor of STAR. Activates SIRT1 transcription under cellular stress conditions. Activates transcription of OSR2. Defects in FOXL2 are a cause of blepharophimosis, ptosis, and epicanthus inversus syndrome (BPES); also known as blepharophimosis syndrome. It is an autosomal dominant disorder characterized by eyelid dysplasia, small palpebral fissures, drooping eyelids and a skin fold running inward and upward from the lower lid. In type I BPSE (BPES1) eyelid abnormalities are associated with female infertility. Affected females show an ovarian deficit due to primary amenorrhea or to premature ovarian failure (POF). In type II BPSE (BPES2) affected individuals show only the eyelid defects. There is a mutational hotspot in the region coding for the poly-Ala domain, since 30% of all mutations in the ORF lead to poly-Ala expansions, resulting mainly in BPES type II. Defects in FOXL2 are a cause of premature ovarian failure type 3 (POF3). An ovarian disorder defined as the cessation of ovarian function under the age of 40 years. It is characterized by oligomenorrhea or amenorrhea, in the presence of elevated levels of serum gonadotropins and low estradiol.

Protein type: Transcription factor; DNA-binding

Chromosomal Location of Human Ortholog: 3q23

Cellular Component: nucleus

Molecular Function: protein binding; DNA binding; ubiquitin conjugating enzyme binding; sequence-specific DNA binding; caspase regulator activity; estrogen receptor binding; transcription factor activity

Biological Process: transcription from RNA polymerase II promoter; embryonic eye morphogenesis; positive regulation of apoptosis; positive regulation of transcription, DNA-dependent; female somatic sex determination; positive regulation of caspase activity; negative regulation of transcription from RNA polymerase II promoter; regulation of transcription from RNA polymerase II promoter; ovarian follicle development; positive regulation of transcription from RNA polymerase II promoter; DNA fragmentation during apoptosis; menstruation; cell differentiation; negative regulation of transcription, DNA-dependent; extraocular skeletal muscle development; granulosa cell differentiation

Disease: Blepharophimosis, Ptosis, And Epicanthus Inversus; Premature Ovarian Failure 3