Rabbit anti-Human, Rat FoxE3 Polyclonal Antibody | anti-FoxE3 antibody

FoxE3 (R97) Polyclonal Antibody

Cat. Num. AAA3002445

• Reactivity: Human, Rat
• Applications: Immunohistochemistry
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: FoxE3; Polyclonal Antibody; FoxE3 (R97) Polyclonal Antibody; Forkhead box protein E3; Forkhead-related protein FKHL12; Forkhead-related transcription factor 8; FREAC-8; FKHL12; FREAC8; FOXE3; anti-FoxE3 antibody

• Host: Rabbit
• Reactivity: Human, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: FoxE3 (R97) polyclonal antibody detects endogenous levels of FoxE3 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 319

• Applicable Applications for anti-FoxE3 antibody: Immunohistochemistry (IHC)
• Application Notes: IHC: 1:50-1:200
• Immunogen: Synthetic peptide, corresponding to amino acids 64-116 of Human FoxE3.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Testing Data

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of FoxE3 (R97) pAb in paraffin-embedded human tonsil carcinoma tissue at 1:50.)

Related Product Information for anti-FoxE3 antibody

The human forkhead-box (FOX) gene family consists of at least 43 members, including FOXE3, a 288-amino acid protein. FOXE3 is a winged-helix transcription factor that plays a crucial role during the initial stages of lens development and closure of the lens vesicle. FOXE3 may also act as a factor that promotes survival and proliferation while preventing differentiation in the lens epithelium. As the posterior cells of the lens fiber begin to differentiate, expression of FOXE3 is limited to the undifferentiated cells coating the anterior surface of the lens. Congenital primary aphakia (CPA) is a rare developmental disorder caused by a null mutation in the FOXE3 gene that is identified by the absence of a lens. The development of CPA is normally stimulated during the fourth or fifth week of human embryogenesis.

NCBI and Uniprot Product Information

• NCBI GI #: 11386197
• NCBI Accession #: NP_036318.1
• NCBI GenBank Nucleotide #: NP_036318.1
• UniProt Accession #: Q13461
• Molecular Weight: ~ 33kDa
• NCBI Official Full Name: forkhead box protein E3
• UniProt Protein Name: Forkhead box protein E3
• Protein Family: Forkhead box protein
• UniProt Gene Name: FOXE3
• UniProt Synonym Gene Names: FKHL12; FREAC8; FREAC-8
• UniProt Entry Name: FOXE3_HUMAN

Uniprot Description

FOXE3: Defects in FOXE3 are a cause of anterior segment mesenchymal dysgenesis (ASMD); also known as anterior segment ocular dysgenesis (ASOD). ASMD consists of a range of developmental defects in structures at the front of the eye, resulting from abnormal migration or differentiation of the neural crest derived mesenchymal cells that give rise to the cornea, iris, and other components of the anterior chamber during eye development. Mature anterior segment anomalies are associated with an increased risk of glaucoma and corneal opacity. Conditions falling within the phenotypic spectrum include aniridia, posterior embryotoxon, Axenfeld anomaly, Reiger anomaly/syndrome, Peters anomaly, and iridogoniodysgenesis. Defects in FOXE3 are a cause of congenital primary aphakia (CPA). Aphakia is a rare congenital eye disorder in which the lens is missing. It has been histologically subdivided into primary and secondary forms, in accordance with the severity of defects of the ocular tissues, whose development requires the initial presence of a lens. CPA results from an early developmental arrest, around the 4th-5th week of gestation in humans, that prevents the formation of any lens structure and leads to severe secondary ocular defects, including a complete aplasia of the anterior segment of the eye. In contrast, in secondary aphakic eyes, lens induction has occurred, and the lens vesicle has developed to some degree but finally has progressively resorbed perinatally, leading, therefore, to less-severe ocular defects.

Protein type: DNA-binding

Chromosomal Location of Human Ortholog: 1p32

Cellular Component: transcription factor complex; nucleus

Molecular Function: sequence-specific DNA binding; transcription factor activity

Biological Process: regulation of transcription from RNA polymerase II promoter; transcription from RNA polymerase II promoter; camera-type eye development; cell development; positive regulation of epithelial cell proliferation

Disease: Anterior Segment Mesenchymal Dysgenesis; Aphakia, Congenital Primary