Western Blot (WB)
(HEK293 lysate (10ug protein in RIPA buffer) over expressing Human FOXC2 with DYKDDDDK tag probed (0.1ug/ml) in Lane A and probed with anti- DYKDDDDK Tag (1/1000) in lane C. Mock-transfected HEK293 probed (1mg/ml) in Lane B. Primary incubations were for 1 hour. Detected by chemiluminescence.)
Goat FOXC2 Polyclonal Antibody | anti-FOXC2 antibody
Goat anti-FOXC2 Antibody
Immunohistochemistry: In paraffin embedded Human Bresast cancer shows nuclear staning with some weak cytoplasm staining. Recommended concentration, 2-4ug/ml.
Western Blot: In transfected HEK293 transiently expressing full-length Human FOXC2 (myc and DYKDDDDK tagged), a band of approx. 65kDa was observed. No bands were observed in mock-transfected HEK293 and the same band was observed using anti-DYKDDDDK tag antibody. Recommended concentration, 0.1-0.2ug/ml.
Western Blot (WB)
(HEK293 lysate (10ug protein in RIPA buffer) over expressing Human FOXC2 with DYKDDDDK tag probed (0.1ug/ml) in Lane A and probed with anti- DYKDDDDK Tag (1/1000) in lane C. Mock-transfected HEK293 probed (1mg/ml) in Lane B. Primary incubations were for 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
(HEK293 lysate (10ug protein in RIPA buffer) over expressing Human FOXC2 with DYKDDDDK tag probed (0.1ug/ml) in Lane A and probed with anti- DYKDDDDK Tag (1/1000) in lane C. Mock-transfected HEK293 probed (1mg/ml) in Lane B. Primary incubations were for 1 hour. Detected by chemiluminescence.)
Immunohistochemistry (IHC)
((2ug/ml) staining of paraffin embedded Human Breast cancer. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.)
Immunohistochemistry (IHC)
((2ug/ml) staining of paraffin embedded Human Breast cancer. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.)
NCBI and Uniprot Product Information
NCBI Description
This gene belongs to the forkhead family of transcription factors which is characterized by a distinct DNA-binding forkhead domain. The specific function of this gene has not yet been determined; however, it may play a role in the development of mesenchymal tissues. [provided by RefSeq, Jul 2008]
Uniprot Description
FOXC2: Transcriptional activator. Might be involved in the formation of special mesenchymal tissues. Defects in FOXC2 are the cause of lymphedema hereditary type 2 (LMPH2); also known as Meige lymphedema. Hereditary lymphedema is a chronic disabling condition which results in swelling of the extremities due to altered lymphatic flow. Patients with lymphedema suffer from recurrent local infections, and physical impairment. Defects in FOXC2 are a cause of lymphedema-yellow nails (LYYN). LYYN is characterized by yellow, dystrophic, thick and slowly growing nails, associated with lymphedema and respiratory involvement. Lymphedema occurs more often in the lower limbs. It can appear at birth or later in life. Onset generally follows the onset of ungual abnormalities. Defects in FOXC2 are a cause of lymphedema-distichiasis (LYD). LYD is characterized by primary limb lymphedema usually starting at puberty (but in some cases later or at birth) and associated with distichiasis (double rows of eyelashes, with extra eyelashes growing from the Meibomian gland orifices).
Protein type: Apoptosis; Cell development/differentiation; DNA-binding; Transcription factor
Chromosomal Location of Human Ortholog: 16q24.1
Cellular Component: nucleus
Molecular Function: chromatin DNA binding; identical protein binding; protein binding; sequence-specific DNA binding; transcription factor activity
Biological Process: heart development; insulin receptor signaling pathway; lymphangiogenesis; negative regulation of transcription from RNA polymerase II promoter; positive regulation of cell adhesion mediated by integrin; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; response to hormone stimulus
Disease: Lymphedema-distichiasis Syndrome
Research Articles on FOXC2
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Product Notes
The FOXC2 foxc2 (Catalog #AAA420369) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-FOXC2 Antibody reacts with Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow and may cross-react with other species as described in the data sheet. AAA Biotech's FOXC2 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB-Trf), Immunohistochemistry (IHC). Peptide ELISA: Antibody detection limit dilution 1: 16000. Immunohistochemistry: In paraffin embedded Human Bresast cancer shows nuclear staning with some weak cytoplasm staining. Recommended concentration, 2-4ug/ml. Western Blot: In transfected HEK293 transiently expressing full-length Human FOXC2 (myc and DYKDDDDK tagged), a band of approx. 65kDa was observed. No bands were observed in mock-transfected HEK293 and the same band was observed using anti-DYKDDDDK tag antibody. Recommended concentration, 0.1-0.2ug/ml. Researchers should empirically determine the suitability of the FOXC2 foxc2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: RHAAPYSYDC TKY. It is sometimes possible for the material contained within the vial of "FOXC2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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