Rabbit FOS / c-FOS Polyclonal Antibody | anti-FOS antibody

Rabbit Polyclonal (IgG) to Human FOS / c-FOS

Cat. Num. AAA247088

• Gene Names: FOS; p55; AP-1; C-FOS
• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry, Western Blot, ELISA
• Purity: Immunoaffinity Purified
• Synonyms: FOS / c-FOS; Polyclonal Antibody; Rabbit Polyclonal (IgG) to Human FOS / c-FOS; Anti-FOS / c-FOS Antibody (aa1-50) IHC-plus; FOS; Activator protein 1; AP-1; Cellular oncogene c-fos; Cellular oncogene fos; Proto-oncogene c-Fos; v-fos; C-FOS; G0s7; p55; Human FOS; c-FOS; anti-FOS antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Fos Antibody detects endogenous levels of total Fos protein.
• Purity/Purification: Immunoaffinity Purified
• Form/Format: PBS (without Mg2+, Ca2+), pH 7.4, 150 mM sodium chloride, 0.02% sodium azide, 50% glycerol
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 380

• Applicable Applications for anti-FOS antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA (1:10000), IHC-P (10 ug/ml), WB (1:500 - 1:1000)
• Target Species: Human
• Immunogen Description: Synthetic peptide from human Fos (aa1-50).
• Immunogen: FOS / c-FOS antibody was raised against synthetic peptide from human Fos (aa1-50).
• Antigen Modification: aa1-50
• Preparation and Storage: Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles.

Immunohistochemistry (IHC)

(Anti-FOS / c-FOS antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)

Western Blot (WB)

(Western blot of extracts from HepG2 cells, using Fos Antibody. The lane on the right is treated with the synthesized peptide.)

Related Product Information for anti-FOS antibody

C-Fos is the product of an immediate early gene. It is a major nuclear target for signal transduction pathways involved in the regulation of cell growth, differentiation, and transformation. The Fos and Jun proteins from dimeric complexes that stimulate transcription of genes containing AP-1 regulatory elements. Studies in rodents have shown that c-Fos is essential in regulating neuronal cell survival versus death.

NCBI and Uniprot Product Information

• NCBI GI #: 4885241
• NCBI GeneID: 2353
• NCBI Accession #: NP_005243.1
• NCBI GenBank Nucleotide #: NM_005252.3
• UniProt Accession #: P01100; P18849; A8K4E2; B4DQ65
• Molecular Weight: 36,301 Da
• NCBI Official Full Name: proto-oncogene c-Fos
• NCBI Official Synonym Full Names: FBJ murine osteosarcoma viral oncogene homolog
• NCBI Official Symbol: FOS
• NCBI Official Synonym Symbols: p55; AP-1; C-FOS
• NCBI Protein Information: proto-oncogene c-Fos; activator protein 1; cellular oncogene c-fos; G0/G1 switch regulatory protein 7; FBJ murine osteosarcoma viral (v-fos) oncogene homolog (oncogene FOS)
• UniProt Protein Name: Proto-oncogene c-Fos
• Protein Family: Proto-oncogene
• UniProt Gene Name: FOS
• UniProt Synonym Gene Names: G0S7
• UniProt Entry Name: FOS_HUMAN

NCBI Description

The Fos gene family consists of 4 members: FOS, FOSB, FOSL1, and FOSL2. These genes encode leucine zipper proteins that can dimerize with proteins of the JUN family, thereby forming the transcription factor complex AP-1. As such, the FOS proteins have been implicated as regulators of cell proliferation, differentiation, and transformation. In some cases, expression of the FOS gene has also been associated with apoptotic cell death. [provided by RefSeq, Jul 2008]

Uniprot Description

Fos: a proto-oncogenic transcription factor of the bZIP family. Dimerizes with proteins of the JUN family, thereby forming the transcription factor complex AP-1. FOS proteins function as regulators of cell proliferation, differentiation, and transformation. In some cases, expression of FOS has also been associated with apoptotic cell death. Expression increases upon a variety of stimuli, including growth factors, cytokines, neurotransmitters, polypeptide hormones, stress and cell injury.

Protein type: Oncoprotein; Transcription factor; DNA-binding; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 14q24.3

Cellular Component: nucleoplasm; transcription factor complex; neuron projection; membrane; endoplasmic reticulum; cytosol; nucleus

Molecular Function: protein binding; double-stranded DNA binding; transcription factor activity; transcription factor binding

Biological Process: transcription from RNA polymerase II promoter; response to gravity; response to cAMP; positive regulation of osteoclast differentiation; response to toxin; positive regulation of transcription, DNA-dependent; stress-activated MAPK cascade; response to lipopolysaccharide; female pregnancy; toll-like receptor 3 signaling pathway; toll-like receptor 10 signaling pathway; toll-like receptor 5 signaling pathway; regulation of transcription factor activity; transforming growth factor beta receptor signaling pathway; conditioned taste aversion; DNA methylation; inflammatory response; toll-like receptor 4 signaling pathway; aging; response to corticosterone stimulus; response to drug; response to light stimulus; nervous system development; MyD88-independent toll-like receptor signaling pathway; sleep; toll-like receptor 2 signaling pathway; cellular response to hormone stimulus; regulation of transcription from RNA polymerase II promoter; MyD88-dependent toll-like receptor signaling pathway; response to mechanical stimulus; response to cytokine stimulus; cellular response to extracellular stimulus; toll-like receptor signaling pathway; innate immune response; positive regulation of transcription from RNA polymerase II promoter; toll-like receptor 9 signaling pathway; response to cold; response to progesterone stimulus