Rabbit anti-Mouse; Rat FNIP1 Polyclonal Antibody | anti-FNIP1 antibody

FNIP1 Antibody: Alkaline Phosphatase

Cat. Num. AAA8000382

• Reactivity: Mouse; Rat
• Applications: Western Blot, Immunocytochemistry, Immunofluorescence
• Purity: Peptide Affinity Purified
• Synonyms: FNIP1; Polyclonal Antibody; FNIP1 Antibody: Alkaline Phosphatase; Rabbit Anti-Human FNIP1 Polyclonal; FNIP1 Antibody; Folliculin-interacting protein 1 Antibody; Folliculin interacting protein 1 Antibody; KIAA1961 Antibody; anti-FNIP1 antibody

• Host: Rabbit
• Reactivity: Mouse; Rat
• Clonality: Polyclonal
• Specificity: Detects ~130 kDa.
• Purity/Purification: Peptide Affinity Purified
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 1138

• Applicable Applications for anti-FNIP1 antibody: Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)
• Application Notes: WB: 1:1000; ICC/IF: 1:100
• Target: FNIP1
• Immunogen: Synthetic peptide from the mid-protein of Human FNIP1
• Immunogen Species: Human
• Cellular Localization: Cytoplasm
• Conjugate: Alkaline Phosphatase
• Storage Buffer: PBS, 50% glycerol, 0.09% sodium azide
• Tissue Specificity: Strong expression is found in the heart, liver placenta, muscle, nasal mucosa, salivary gland and uvula and moderate expression in kidney and lung. Higher levels detected in clear cell renal cell carcinoma (RCC) and chromophobe RCC than in normal kidney tissue.
• Preparation and Storage: Store at -20 degree C.

Immunocytochemistry/Immunofluorescence (ICC/IF)

(Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-FNIP1 Polyclonal Antibody (SPC-718). Tissue: C2C12 Cells (Mouse Myoblast cell line). Species: Mouse. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-FNIP1 Polyclonal Antibody (SPC-718) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm . Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) FNIP1 Antibody (D) Composite.)

Western Blot (WB)

(Western blot analysis of Mouse, Rat Kidney showing detection of ~131 kDa FNIP1 protein using Rabbit Anti-FNIP1 Polyclonal Antibody (SPC-718). Lane 1: Molecular Weight Ladder (MW). Lane 2: Mouse Kidney cell lysates. Lane 3: Rat Kidney cell lysates. Load: 20 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-FNIP1 Polyclonal Antibody (SPC-718) at 1:1000 for 16 hours at 4 degree C. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min at RT. Predicted/Observed Size: ~131 kDa.)

Product Categories/Family for anti-FNIP1 antibody

Antibodies; Cancer; Cell Cycle; mTOR Pathway; Cancer; Autophagy

NCBI and Uniprot Product Information

• NCBI GI #: 120953264
• NCBI GeneID: 96459
• NCBI Accession #: NP_001008738.2
• NCBI GenBank Nucleotide #: NM_001008738.2
• UniProt Accession #: Q8TF40; Q86T47; Q9BUT0; D6RJH5
• Molecular Weight: 127,444 Da
• NCBI Official Full Name: folliculin-interacting protein 1 isoform 2
• NCBI Official Synonym Full Names: folliculin interacting protein 1
• NCBI Official Symbol: FNIP1
• NCBI Protein Information: folliculin-interacting protein 1
• UniProt Protein Name: Folliculin-interacting protein 1
• Protein Family: Folliculin-interacting protein
• UniProt Gene Name: FNIP1
• UniProt Synonym Gene Names: KIAA1961

NCBI Description

This gene encodes a protein that binds to the tumor suppressor protein folliculin and to AMP-activated protein kinase (AMPK). The encoded protein participates in the regulation of cellular metabolism and nutrient sensing by modulating the AMPK and target of rapamycin signaling pathways. This gene has a closely related paralog that encodes a protein with similar binding activities. Both related proteins also associate with the molecular chaperone heat shock protein-90 (Hsp90) and negatively regulate its ATPase activity and facilitate its association with folliculin. [provided by RefSeq, Jul 2017]

Uniprot Description

Acts as a co-chaperone of HSP90AA1. Inhibits the ATPase activity of HSP90AA1 leading to reduction in its chaperone activity. Facilitates the binding of client protein FLCN to HSP90AA1. Competes with the activating co-chaperone AHSA1 for binding to HSP90AA1, thereby providing a reciprocal regulatory mechanism for chaperoning of client proteins (PubMed:27353360). May be involved in energy and/or nutrient sensing through the AMPK and mTOR signaling pathways (PubMed:17028174). May regulate phosphorylation of RPS6KB1 (PubMed:18663353).