Rabbit anti-Human FMO3 Polyclonal Antibody | anti-FMO3 antibody

FMO3 Rabbit pAb

Cat. Num. AAA4755077

• Gene Names: FMO3; TMAU; FMOII; dJ127D3.1
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry, ELISA
• Purity: Affinity purified
• Synonyms: FMO3; Polyclonal Antibody; FMO3 Rabbit pAb; Dimethylaniline monooxygenase [N-oxide-forming] 3; Dimethylaniline oxidase 3; FMO II; FMO form 2; Hepatic flavin-containing monooxygenase 3; FMO 3; Trimethylamine monooxygenase; anti-FMO3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: FMO3 Polyclonal Antibody detects endogenous levels of FMO3 protein.
• Purity/Purification: Affinity purified
• Form/Format: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-FMO3 antibody: Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), ELISA (EIA)
• Application Notes: WB: 1:500-1:2000; IHC-P: 1:100-1:300; ELISA: 1:20,000
• Immunogen: The antiserum was produced against synthesized peptide derived from the Internal region of human FMO3. AA range:101-150
• Conjugation: Unconjugated
• Nucleotide Accession: 2328
• Modification: Unmodified
• Cell Position: Endoplasmic reticulum, Membrane, Microsome
• Preparation and Storage: Store at 4 degree C for short term. Store at -20 degree C for long term. Avoid freeze/thaw cycle.

Western Blot (WB)

(Western Blot analysis of HeLa cells using FMO3 Polyclonal Antibody. Secondary antibody was diluted at 1:20000)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human-liver, antibody was diluted at 1:100)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human-liver, antibody was diluted at 1:100)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human-lung, antibody was diluted at 1:100)

Product Categories/Family for anti-FMO3 antibody

Primary antibody

NCBI and Uniprot Product Information

• NCBI GI #: 6166183
• NCBI GeneID: 2328
• UniProt Accession #: P31513; Q14854; Q8N5N5; B2R816
• Molecular Weight: 532
• NCBI Official Full Name: Dimethylaniline monooxygenase
• NCBI Official Synonym Full Names: flavin containing monooxygenase 3
• NCBI Official Symbol: FMO3
• NCBI Official Synonym Symbols: TMAU; FMOII; dJ127D3.1
• NCBI Protein Information: dimethylaniline monooxygenase [N-oxide-forming] 3; dimethylaniline monooxygenase [N-oxide-forming] 3; FMO 3; FMO II; FMO form 2; dimethylaniline oxidase 3; trimethylamine monooxygenase; hepatic flavin-containing monooxygenase 3; hepatic flavin-containing
• UniProt Protein Name: Dimethylaniline monooxygenase [N-oxide-forming] 3
• Protein Family: Dimethylaniline monooxygenase
• UniProt Gene Name: FMO3
• UniProt Synonym Gene Names: FMO 3
• UniProt Entry Name: FMO3_HUMAN

NCBI Description

Flavin-containing monooxygenases (FMO) are an important class of drug-metabolizing enzymes that catalyze the NADPH-dependent oxygenation of various nitrogen-,sulfur-, and phosphorous-containing xenobiotics such as therapeutic drugs, dietary compounds, pesticides, and other foreign compounds. The human FMO gene family is composed of 5 genes and multiple pseudogenes. FMO members have distinct developmental- and tissue-specific expression patterns. The expression of this FMO3 gene, the major FMO expressed in adult liver, can vary up to 20-fold between individuals. This inter-individual variation in FMO3 expression levels is likely to have significant effects on the rate at which xenobiotics are metabolised and, therefore, is of considerable interest to the pharmaceutical industry. This transmembrane protein localizes to the endoplasmic reticulum of many tissues. Alternative splicing of this gene results in multiple transcript variants encoding the same protein. Mutations in this gene cause the disorder trimethylaminuria (TMAu) which is characterized by the accumulation and excretion of unmetabolized trimethylamine and a distinctive body odor. In healthy individuals, trimethylamine is primarily converted to the non odorous trimethylamine N-oxide.[provided by RefSeq, Aug 2009]

Uniprot Description

FMO3: Involved in the oxidative metabolism of a variety of xenobiotics such as drugs and pesticides. It N-oxygenates primary aliphatic alkylamines as well as secondary and tertiary amines. Plays an important role in the metabolism of trimethylamine (TMA), via the production of TMA N-oxide (TMAO). Is also able to perform S-oxidation when acting on sulfide compounds. Defects in FMO3 are the cause of trimethylaminuria (TMAU); also known as fish-odor syndrome. TMAU is an inborn error of metabolism associated with an offensive body odor and caused by deficiency of FMO-mediated N-oxidation of amino- trimethylamine (TMA) derived from foodstuffs. Such individuals excrete relatively large amounts of TMA in their urine, sweat, and breath, and exhibit a fishy body odor characteristic of the malodorous free amine. Belongs to the FMO family.

Protein type: EC 1.14.13.8; EC 1.14.13.148; Xenobiotic Metabolism - drug metabolism - cytochrome P450; Oxidoreductase

Chromosomal Location of Human Ortholog: 1q24.3

Cellular Component: endoplasmic reticulum membrane; intracellular membrane-bound organelle; integral to membrane

Molecular Function: amino acid binding; FAD binding; NADP binding; flavin-containing monooxygenase activity

Biological Process: xenobiotic metabolic process; drug metabolic process

Disease: Trimethylaminuria