Rabbit anti-Human FMO3 Polyclonal Antibody | anti-FMO3 antibody

FMO3, NT (Dimethylaniline Monooxygenase [N-oxide-forming] 3, Hepatic Flavin-containing Monooxygenase 3, FMO 3, FMO Form 2, FMO II, Dimethylaniline Oxidase 3) (Biotin)

Cat. Num. AAA6476987

• Gene Names: FMO3; TMAU; FMOII; dJ127D3.1
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Immunoaffinity chromatography.
• Synonyms: FMO3; Polyclonal Antibody; NT (Dimethylaniline Monooxygenase [N-oxide-forming] 3; Hepatic Flavin-containing Monooxygenase 3; FMO 3; FMO Form 2; FMO II; Dimethylaniline Oxidase 3) (Biotin); anti-FMO3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human FMO3.
• Purity/Purification: Purified by Immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
• Sequence Length: 532

• Applicable Applications for anti-FMO3 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-1:500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the N-terminal region of human FMO3 (KLH).
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of FMO3 Antibody (N-term) in MDA-MB231 cell line lysates (35ug/lane). FMO3 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with FMO3 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(FMO3 Antibody (N-term) flow cytometric analysis of MDA-MB231 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-FMO3 antibody

Antibodies; Enzymes; Oxidase

References

1. Allerston, C.K., et.al., Mol. Genet. Metab. 98(1-2), 198-202 (2009).

NCBI and Uniprot Product Information

• NCBI GI #: 50541965
• NCBI GeneID: 2328
• NCBI Accession #: NP_008825.4
• NCBI GenBank Nucleotide #: NP_008825.4
• UniProt Accession #: P31513
• Molecular Weight: 60.033
• NCBI Official Full Name: dimethylaniline monooxygenase
• NCBI Official Synonym Full Names: flavin containing dimethylaniline monoxygenase 3
• NCBI Official Symbol: FMO3
• NCBI Official Synonym Symbols: TMAU; FMOII; dJ127D3.1
• NCBI Protein Information: dimethylaniline monooxygenase [N-oxide-forming] 3
• UniProt Protein Name: Dimethylaniline monooxygenase [N-oxide-forming] 3
• Protein Family: Dimethylaniline monooxygenase
• UniProt Gene Name: FMO3
• UniProt Synonym Gene Names: FMO 3
• UniProt Entry Name: FMO3_HUMAN

NCBI Description

Flavin-containing monooxygenases (FMO) are an important class of drug-metabolizing enzymes that catalyze the NADPH-dependent oxygenation of various nitrogen-,sulfur-, and phosphorous-containing xenobiotics such as therapeutic drugs, dietary compounds, pesticides, and other foreign compounds. The human FMO gene family is composed of 5 genes and multiple pseudogenes. FMO members have distinct developmental- and tissue-specific expression patterns. The expression of this FMO3 gene, the major FMO expressed in adult liver, can vary up to 20-fold between individuals. This inter-individual variation in FMO3 expression levels is likely to have significant effects on the rate at which xenobiotics are metabolised and, therefore, is of considerable interest to the pharmaceutical industry. This transmembrane protein localizes to the endoplasmic reticulum of many tissues. Alternative splicing of this gene results in multiple transcript variants encoding different isoforms. Mutations in this gene cause the disorder trimethylaminuria (TMAu) which is characterized by the accumulation and excretion of unmetabolized trimethylamine and a distinctive body odor. In healthy individuals, trimethylamine is primarily converted to the non odorous trimethylamine N-oxide.[provided by RefSeq, Jan 2016]

Uniprot Description

FMO3: Involved in the oxidative metabolism of a variety of xenobiotics such as drugs and pesticides. It N-oxygenates primary aliphatic alkylamines as well as secondary and tertiary amines. Plays an important role in the metabolism of trimethylamine (TMA), via the production of TMA N-oxide (TMAO). Is also able to perform S-oxidation when acting on sulfide compounds. Defects in FMO3 are the cause of trimethylaminuria (TMAU); also known as fish-odor syndrome. TMAU is an inborn error of metabolism associated with an offensive body odor and caused by deficiency of FMO-mediated N-oxidation of amino- trimethylamine (TMA) derived from foodstuffs. Such individuals excrete relatively large amounts of TMA in their urine, sweat, and breath, and exhibit a fishy body odor characteristic of the malodorous free amine. Belongs to the FMO family.

Protein type: EC 1.14.13.8; EC 1.14.13.148; Xenobiotic Metabolism - drug metabolism - cytochrome P450; Oxidoreductase

Chromosomal Location of Human Ortholog: 1q24.3

Cellular Component: endoplasmic reticulum membrane; intracellular membrane-bound organelle; integral to membrane

Molecular Function: amino acid binding; FAD binding; NADP binding; flavin-containing monooxygenase activity

Biological Process: xenobiotic metabolic process; drug metabolic process

Disease: Trimethylaminuria