Rabbit Flotillin-2 Polyclonal Antibody | anti-R144 antibody

Flotillin-2 (R144) Polyclonal Antibody

Cat. Num. AAA3003153

• Gene Names: FLOT2; ESA; ECS1; ESA1; ECS-1; M17S1
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: Flotillin-2; Polyclonal Antibody; Flotillin-2 (R144) Polyclonal Antibody; Epidermal surface antigen; ESA; Membrane component chromosome 17 surface marker 1; ESA1; M17S1; FLOT2; anti-R144 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Flotillin-2 (R144) polyclonal antibody detects endogenous levels of Flotillin-2 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 428

• Applicable Applications for anti-R144 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: Synthetic peptide, corresponding to amino acids 360-418 of Human TFDP1.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of FLOT2 pAb at 1:500 dilutionLane1:A375 whole cell lysate (40ug) Lane2:SCC-7 whole cell lysate (40ug) Lane3:The Brain tissue lysate of Mouse (40ug) Lane4:C6 whole cell lysate (40ug))

Related Product Information for anti-R144 antibody

Flotillins belong to a family of lipid raft-associated integral membrane proteins that carry a evolutionarily conserved domain called the prohibitin homology domain (PHB). Flotillin members are ubiquitously expressed and located to non-caveolar microdomains (lipid rafts) on the cell plasma membrane where they support signal transduction and regulate lipid raft motility and localization. Two flotillin members have been described, flotillin-1 and flotillin-2. In addition to its colocalization with lipid rafts on the plasma membrane, flotillin-1 also has been found at compartments of the endocytic and autophagosomal pathways, such as recycling/late endosomes, the Golgi complex, as well as the nucleus. Flotillin-2 is mainly localized to the plasma membrane and is prevalent in cell-cell contact sites. However, overexpressed flotillin-2 has also been found in the late endosome. Both flotillin-1 and flotillin-2 are commonly used as lipid raft associated markers.

NCBI and Uniprot Product Information

• NCBI GI #: 254763294
• NCBI GeneID: 2319
• UniProt Accession #: Q14254
• Molecular Weight: ~ 49kDa
• NCBI Official Full Name: Flotillin-2
• NCBI Official Synonym Full Names: flotillin 2
• NCBI Official Symbol: FLOT2
• NCBI Official Synonym Symbols: ESA; ECS1; ESA1; ECS-1; M17S1
• NCBI Protein Information: flotillin-2
• UniProt Protein Name: Flotillin-2
• Protein Family: Flotillin
• UniProt Gene Name: FLOT2
• UniProt Synonym Gene Names: ESA1; M17S1; ESA
• UniProt Entry Name: FLOT2_HUMAN

NCBI Description

Caveolae are small domains on the inner cell membrane involved in vesicular trafficking and signal transduction. This gene encodes a caveolae-associated, integral membrane protein, which is thought to function in neuronal signaling. [provided by RefSeq, Jul 2008]

Uniprot Description

FLOT2: May act as a scaffolding protein within caveolar membranes, functionally participating in formation of caveolae or caveolae-like vesicles. May be involved in epidermal cell adhesion and epidermal structure and function. Heterooligomeric complex of flotillin-1 and flotillin-2 and caveolin-1 and caveolin-2. Interacts with ECM29. In skin, expressed in epidermis and epidermal appendages but not in dermis. Expressed in all layers of the epidermis except the basal layer. In hair follicles, expressed in the suprabasal layer but not the basal layer. Also expressed in melanoma and carcinoma cell lines, fibroblasts and foreskin melanocytes. Belongs to the band 7/mec-2 family. Flotillin subfamily.

Protein type: Cell adhesion

Chromosomal Location of Human Ortholog: 17q11-q12

Cellular Component: focal adhesion; membrane; endocytic vesicle; perinuclear region of cytoplasm; plasma membrane; caveola; flotillin complex; vesicle; endosome; acrosomal membrane

Molecular Function: ionotropic glutamate receptor binding; protein binding; protease binding

Biological Process: epidermis development; protein stabilization; cell adhesion