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Western Blot (WB) (Western Blot (WB) analysis of Flotillin-1 polyclonal antibody)

Rabbit Flotillin-1 Polyclonal Antibody | anti-FLOT1 antibody

Flotillin-1 Polyclonal Antibody

Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunofluorescence
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Synonyms
Flotillin-1; Polyclonal Antibody; Flotillin-1 Polyclonal Antibody; FLOT 1; FLOT1; FLOT1_HUMAN; Flotillin1; Integral membrane component of caveolae; Reggie 2; anti-FLOT1 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
Flotillin-1 polyclonal antibody detects endogenous levels of Flotillin-1 protein.
Purity/Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Form/Format
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Sequence Length
427
Applicable Applications for anti-FLOT1 antibody
Western Blot (WB), Immunofluorescence (IF)
Application Notes
WB: 1:500-1:2000
IF: 1:10-1:100
Immunogen
Recombinant full length Human Flotillin-1.
Preparation and Storage
Store at 4 degree C short term.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of Flotillin-1 polyclonal antibody)

Western Blot (WB) (Western Blot (WB) analysis of Flotillin-1 polyclonal antibody)
Related Product Information for anti-FLOT1 antibody
Lipid rafts are sphingolipid- and cholesterol-rich membrane microdomains that are insoluble in nonionic detergents. Lipid rafts are important for numerous cellular processes, including signal transduction, membrane trafficking and molecular sorting. Flotillins are lipid raft components in neurons and caveloae-associated proteins in A498 kidney cells. Flotillin-1 belongs to the band 7.2/stomatin protein family, whose members are characterized by the presence of a hydrophobic N-terminal region that is predicted to form a single, outside to inside, transmembrane domain. Flotillin-1 and -2 have complementary tissue distributions and their expression levels are independently regulated. At the cellular level, Flotillin-2 is ubiquitously expressed, whereas Flotillin-1 is expressed in A498 kidney cells, muscle cell lines and fibroblasts. Flotillins form a ternary complex with CAP and Cbl, directing the localization of the CAP-Cbl complex to a lipid raft subdomain of the plasma membrane. Association of ER-X with Flotillin localizes ER-X within plasma membrane caveloae and mediates rapid oestrogen activation of the MAP kinase cascade. The expression of the flotillins is also correlated to the progression of Alzhemier pathology.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
~ 47kDa
NCBI Official Full Name
Flotillin-1
NCBI Official Synonym Full Names
flotillin 1
NCBI Official Symbol
FLOT1
NCBI Protein Information
flotillin-1
UniProt Protein Name
Flotillin-1
Protein Family
UniProt Gene Name
FLOT1
UniProt Entry Name
FLOT1_HUMAN

NCBI Description

This gene encodes an protein that localizes to the caveolae, which are small domains on the inner cell membranes. This protein plays a role in vesicle trafficking and cell morphology. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2016]

Uniprot Description

FLOT1: May act as a scaffolding protein within caveolar membranes, functionally participating in formation of caveolae or caveolae-like vesicles. Heterooligomeric complex of flotillin-1 and flotillin-2 and caveolin-1 and caveolin-2. Interacts with ECM29. Belongs to the band 7/mec-2 family. Flotillin subfamily.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 6p21.3

Cellular Component: signalosome; focal adhesion; membrane; lysosomal membrane; integral to membrane; melanosome; microtubule organizing center; caveola; flotillin complex; sarcolemma; endosome; lipid raft

Molecular Function: ionotropic glutamate receptor binding; protein binding; protease binding

Biological Process: extracellular matrix disassembly; axonogenesis; regulation of Rho protein signal transduction

Research Articles on FLOT1

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Product Notes

The FLOT1 flot1 (Catalog #AAA3011404) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Flotillin-1 Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Flotillin-1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF). WB: 1:500-1:2000 IF: 1:10-1:100. Researchers should empirically determine the suitability of the FLOT1 flot1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Flotillin-1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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