Rabbit FLNB Polyclonal Antibody | anti-FLNB antibody

FLNB Polyclonal Antibody

Cat. Num. AAA127703

• Gene Names: FLNB; AOI; FH1; SCT; TAP; LRS1; TABP; FLN-B; FLN1L; ABP-278; ABP-280
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunofluorescence
• Purity: Affinity Purification
• Synonyms: FLNB; Polyclonal Antibody; FLNB Polyclonal Antibody; ABP-278; AOI; DKFZp686A1668; DKFZp686O033; FH1; FLN1L; LRS1; SCT; TABP; TAP; anti-FLNB antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity Purification
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 2602

• Applicable Applications for anti-FLNB antibody: Western Blot (WB), Immunofluorescence (IF)
• Application Notes: WB: 1:500 - 1:1000; IF: 1:20 - 1:50
• Species: Human
• Route: Synthetic Peptide
• Immunogen: A synthetic peptide of human FLNB
• Calculated Molecular Weight: 278kDa
• Preparation and Storage: Store at -20 degree C (regular) or -80 degree C (long term). Avoid freeze / thaw cycles.; Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using FLNB antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded rat brain using FLNB antibody at dilution of 1:100 (40x lens).)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded rat heart using FLNB antibody at dilution of 1:100 (40x lens).)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human liver injury using FLNB antibody at dilution of 1:100 (40x lens).)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human prostate using FLNB antibody at dilution of 1:100 (40x lens).)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human esophagus using FLNB antibody at dilution of 1:100 (40x lens).)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human stomach using FLNB antibody at dilution of 1:100 (40x lens).)

Related Product Information for anti-FLNB antibody

Filamins are a family of dimeric actin binding proteins that function as structural components of cell adhesion sites. They also serve as a scaffold for subcellular targeting of signaling molecules (1). The actin binding domain (alpha-actinin domain) located at the amino terminus is followed by as many as 24 tandem repeats of about 96 residues and the dimerization domain is located at the carboxy terminus. In addition to actin filaments, filamins associate with other structural and signaling molecules such as beta integrins, Rho/Rac/Cdc42, PKC, and the insulin receptor, primarily through the carboxy-terminal dimerization domain (1-3). Filamin A, the most abundant, and filamin B are widely expressed isoforms, while filamin C is predominantly expressed in muscle (1). Filamin A is phosphorylated by PAK1 at Ser2152, which is required for PAK1-mediated actin cytoskeleton reorganization (4).

Product Categories/Family for anti-FLNB antibody

Polyclonal

NCBI and Uniprot Product Information

• NCBI GI #: 296434507
• NCBI GeneID: 2317
• UniProt Accession #: O75369; Q13706; Q59EC2; Q60FE7; Q6MZJ1; Q8WXS9; Q8WXT0; B2ZZ83; B2ZZ84; B2ZZ85; C9JKE6; C9JMC4
• Molecular Weight: 2602
• NCBI Official Full Name: Filamin-B
• NCBI Official Synonym Full Names: filamin B, beta
• NCBI Official Symbol: FLNB
• NCBI Official Synonym Symbols: AOI; FH1; SCT; TAP; LRS1; TABP; FLN-B; FLN1L; ABP-278; ABP-280
• NCBI Protein Information: filamin-B; filamin-3; beta-filamin; ABP-280 homolog; filamin homolog 1; thyroid autoantigen; actin binding protein 278; actin-binding-like protein; Larsen syndrome 1 (autosomal dominant)
• UniProt Protein Name: Filamin-B
• Protein Family: Filamin
• UniProt Gene Name: FLNB
• UniProt Synonym Gene Names: FLN1L; FLN3; TABP; TAP; FLN-B; Fh1; Truncated ABP
• UniProt Entry Name: FLNB_HUMAN

NCBI Description

This gene encodes a member of the filamin family. The encoded protein interacts with glycoprotein Ib alpha as part of the process to repair vascular injuries. The platelet glycoprotein Ib complex includes glycoprotein Ib alpha, and it binds the actin cytoskeleton. Mutations in this gene have been found in several conditions: atelosteogenesis type 1 and type 3; boomerang dysplasia; autosomal dominant Larsen syndrome; and spondylocarpotarsal synostosis syndrome. Multiple alternatively spliced transcript variants that encode different protein isoforms have been described for this gene. [provided by RefSeq, Nov 2009]

Uniprot Description

FLNB: a ubiquitous cytoskeletal protein that links actin filaments to membrane glycoproteins. Six alternatively spliced isoforms have been described. Various interactions and localizations of isoforms affect myotube morphology and myogenesis. Isoforms 1 and 2 are expressed in placenta, bone marrow, brain, umbilical vein endothelial cells, retina and skeletal muscle. Isoform 1 is predominantly expressed in prostate, uterus, liver, thyroid, stomach, lymph node, small intestine, spleen, skeletal muscle, kidney, placenta, pancreas, heart, lung, platelets, endothelial cells, megakaryocytic and erythroleukemic cell lines. Isoform 2 is predominantly expressed in spinal cord and platelets. Also expressed in thyroid adenoma, neurofibrillary tangles (NFT), senile plaques in the hippocampus and cerebral cortex in Alzheimer's disease. Isoform 3 and isoform 6 are expressed predominantly in lung, heart, skeletal muscle, testis, spleen, thymus and leukocytes. Isoform 4 and isoform 5 are expressed in heart.

Protein type: Motility/polarity/chemotaxis; Actin-binding

Chromosomal Location of Human Ortholog: 3p14.3

Cellular Component: focal adhesion; cytoplasm; integral to membrane; stress fiber; plasma membrane; cell cortex; Z disc; cytosol; actin cytoskeleton

Molecular Function: identical protein binding; protein binding; actin binding

Biological Process: skeletal muscle development; cytoskeletal anchoring; cytokine and chemokine mediated signaling pathway; actin cytoskeleton organization and biogenesis; signal transduction; cell differentiation

Disease: Larsen Syndrome; Boomerang Dysplasia; Atelosteogenesis, Type I; Spondylocarpotarsal Synostosis Syndrome; Atelosteogenesis, Type Iii