Rabbit FLIP delta + gamma Polyclonal Antibody | anti-CFLAR antibody

FLIP Antibody

Cat. Num. AAA9612492

• Gene Names: CFLAR; CASH; FLIP; MRIT; CLARP; FLAME; cFLIP; Casper; FLAME1; c-FLIP; FLAME-1; I-FLICE; c-FLIPL; c-FLIPR; c-FLIPS; CASP8AP1
• Reactivity: Human; Predicted Reactivity: Pig(83%), Horse(100%), Dog(100%)
• Applications: ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Synonyms: FLIP delta + gamma; Polyclonal Antibody; FLIP Antibody; c FLIP; c FLIPL; c FLIPR; c FLIPS; c-FLIP; c-FLIPL; c-FLIPR; c-FLIPS; CASH; CASP8 and FADD like apoptosis regulator; CASP8 and FADD-like apoptosis regulator subunit p12; CASP8AP1; Caspase homolog; Caspase like apoptosis regulatory protein; Caspase related inducer of apoptosis; Caspase-eight-related protein; Caspase-like apoptosis regulatory protein; Casper; Cellular FLICE-like inhibitory protein; CFLA; Cflar; CFLAR_HUMAN; CLARP; FADD like anti apoptotic molecule; FADD-like antiapoptotic molecule 1; FLAME; FLAME-1; FLAME1; FLIP; I FLICE; I-FLICE; Inhibitor of FLICE; MACH-related inducer of toxicity; MRIT; OTTHUMP00000163715; OTTHUMP00000206475; OTTHUMP00000206476; OTTHUMP00000206478; OTTHUMP00000206479; OTTHUMP00000206480; OTTHUMP00000206482; OTTHUMP00000207360; Usurpin; Usurpin beta; anti-CFLAR antibody

• Host: Rabbit
• Reactivity: Human; Predicted Reactivity: Pig(83%), Horse(100%), Dog(100%)
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: FLIP Antibody detects endogenous levels of total FLIP.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 480

• Applicable Applications for anti-CFLAR antibody: ELISA (EIA)
• Application Notes: WB: 1:500-1:2000; IF: 1:100-1:500; ICC: 1:100-1:500; ELISA(peptide): 1:20,000-1:40,000
• Immunogen: A synthesized peptide derived from human FLIP, corresponding to a region within the internal amino acids.
• Fragment: Fab fragment
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of extracts from K562, using FLIP delta + gamma Antibody. The lane on the left was treated with blocking peptide.)

Immunofluorescence (IF)/Immunocytochemistry (ICC)

(MBS9612492 staining A549 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(MBS9612492) and mouse anti-beta tubulin Ab(T0023) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)

Related Product Information for anti-CFLAR antibody

Function: Apoptosis regulator protein which may function as a crucial link between cell survival and cell death pathways in mammalian cells. Acts as an inhibitor of TNFRSF6 mediated apoptosis. A proteolytic fragment (p43) is likely retained in the death-inducing signaling complex (DISC) thereby blocking further recruitment and processing of caspase-8 at the complex. Full length and shorter isoforms have been shown either to induce apoptosis or to reduce TNFRSF-triggered apoptosis. Lacks enzymatic (caspase) activity.

Post Translational Modifications: Proteolytically processed by CASP8 generating subunit p43 and p12.

Tissue Specificity: Widely expressed. Higher expression in skeletal muscle, pancreas, heart, kidney, placenta, and peripheral blood leukocytes. Also detected in diverse cell lines. Isoform 8 is predominantly expressed in testis and skeletal muscle.

Subunit Structure: TNFRSF6 stimulation triggers recruitment to the death-inducing signaling complex (DISC) formed by TNFRSF6, FADD and CASP8 (PubMed:9880531). A proteolytic fragment (p43) stays associated with the DISC (PubMed:9880531). Also interacts with FADD, CASP8, CASP3, TRAF1, TRAF2 and Bcl-X(L) (in vitro) (PubMed:9326610, PubMed:9208847). Interacts with RIPK1 (By similarity) (PubMed:9326610, PubMed:9208847, PubMed:9880531).(Microbial infection) Interacts with HBV protein X.

Similarity: The caspase domain lacks the active site residues involved in catalysis.Belongs to the peptidase C14A family.

NCBI and Uniprot Product Information

• NCBI GI #: 12643547
• NCBI GeneID: 8837
• UniProt Accession #: O15519
• Molecular Weight: Observed Molecular Weight: (Observed)55kD.; Predicted Molecular Weight: (Calculated)55kDa.
• NCBI Official Full Name: CASP8 and FADD-like apoptosis regulator
• NCBI Official Synonym Full Names: CASP8 and FADD like apoptosis regulator
• NCBI Official Symbol: CFLAR
• NCBI Official Synonym Symbols: CASH; FLIP; MRIT; CLARP; FLAME; cFLIP; Casper; FLAME1; c-FLIP; FLAME-1; I-FLICE; c-FLIPL; c-FLIPR; c-FLIPS; CASP8AP1
• NCBI Protein Information: CASP8 and FADD-like apoptosis regulator
• UniProt Protein Name: CASP8 and FADD-like apoptosis regulator
• Protein Family: CASP8 and FADD-like apoptosis regulator
• UniProt Gene Name: CFLAR
• UniProt Synonym Gene Names: CASH; CASP8AP1; CLARP; MRIT; CASH; Casper; CLARP; c-FLIP; FLAME-1; I-FLICE; MRIT
• UniProt Entry Name: CFLAR_HUMAN

NCBI Description

The protein encoded by this gene is a regulator of apoptosis and is structurally similar to caspase-8. However, the encoded protein lacks caspase activity and appears to be itself cleaved into two peptides by caspase-8. Several transcript variants encoding different isoforms have been found for this gene, and partial evidence for several more variants exists. [provided by RefSeq, Feb 2011]