Human Antithrombin receptor ELISA Kit | ATR elisa kit

Human Antithrombin receptor ELISA Kit

Cat. Num. AAA733669

• Gene Names: ATR; FRP1; MEC1; SCKL; FCTCS; SCKL1
• Reactivity: Human
• Synonyms: Antithrombin receptor; Human Antithrombin receptor ELISA Kit; ATR elisa kit

• Reactivity: Human

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Detection Range: 5.0-100ng/mL
• Sensitivity: 1.0ng/mL
• Intended Uses: This ATR ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human ATR. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ATR elisa kit

Principle of the Assay: ATR ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ATR antibody and an ATR-HRP conjugate. The assay sample and buffer are incubated together with ATR-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ATR concentration since ATR from samples and ATR-HRP conjugate compete for the anti-ATR antibody binding site. Since the number of sites is limited, as more sites are occupied by ATR from the sample, fewer sites are left to bind ATR-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ATR concentration in each sample is interpolated from this standard curve.

Product Categories/Family for ATR elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 1653996
• NCBI GeneID: 545
• UniProt Accession #: Q13535; Q59HB2; Q7KYL3; Q93051; Q9BXK4
• Molecular Weight: 297,479 Da
• NCBI Official Full Name: atr
• NCBI Official Synonym Full Names: ATR serine/threonine kinase
• NCBI Official Symbol: ATR
• NCBI Official Synonym Symbols: FRP1; MEC1; SCKL; FCTCS; SCKL1
• NCBI Protein Information: serine/threonine-protein kinase ATR; FRAP-related protein-1; MEC1, mitosis entry checkpoint 1, homolog; ataxia telangiectasia and Rad3-related protein
• UniProt Protein Name: Serine/threonine-protein kinase ATR
• UniProt Gene Name: ATR
• UniProt Synonym Gene Names: FRP1
• UniProt Entry Name: ATR_HUMAN

NCBI Description

The protein encoded by this gene belongs the PI3/PI4-kinase family, and is most closely related to ATM, a protein kinase encoded by the gene mutated in ataxia telangiectasia. This protein and ATM share similarity with Schizosaccharomyces pombe rad3, a cell cycle checkpoint gene required for cell cycle arrest and DNA damage repair in response to DNA damage. This kinase has been shown to phosphorylate checkpoint kinase CHK1, checkpoint proteins RAD17, and RAD9, as well as tumor suppressor protein BRCA1. Mutations of this gene are associated with Seckel syndrome. An alternatively spliced transcript variant of this gene has been reported, however, its full length nature is not known. Transcript variants utilizing alternative polyA sites exist. [provided by RefSeq, Jul 2008]

Uniprot Description

ATR: a ser/thr kinase of the PIKK family most closely related to ATM. Activates checkpoint signaling upon genotoxic stresses such as ionizing radiation (IR), ultraviolet light (UV), or DNA replication stalling, thereby acting as a DNA damage sensor. Recognizes the substrate consensus sequence [ST]Q. Phosphorylates BRCA1, Chk1, MCM2, RAD17, RPA2, SMC1 and p53, which collectively inhibit DNA replication and mitosis and promote DNA repair, recombination and apoptosis. Phosphorylates 'Ser-139' of histone variant H2AX/H2AFX at sites of DNA damage, thereby regulating DNA damage response mechanism. Required for FANCD2 ubiquitination. Critical for maintenance of fragile site stability and efficient regulation of centrosome duplication.

Protein type: Membrane protein, integral; Kinase, protein; EC 2.7.11.1; Protein kinase, atypical; Kinase, lipid; Protein kinase, Ser/Thr (non-receptor); ATYPICAL group; PIKK family; ATR subfamily

Chromosomal Location of Human Ortholog: 3q23

Cellular Component: nucleoplasm; XY body; Golgi apparatus; PML body; chromosome

Molecular Function: protein dimerization activity; protein serine/threonine kinase activity; protein binding; DNA binding; MutLalpha complex binding; MutSalpha complex binding; ATP binding; protein kinase activity

Biological Process: response to drug; peptidyl-serine phosphorylation; negative regulation of DNA replication; protein amino acid autophosphorylation; regulation of protein binding; multicellular organismal development; DNA damage checkpoint; positive regulation of DNA damage response, signal transduction by p53 class mediator; DNA repair; DNA replication; cell cycle; response to DNA damage stimulus; double-strand break repair via homologous recombination

Disease: Seckel Syndrome 1; Cutaneous Telangiectasia And Cancer Syndrome, Familial