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Western Blot (WB) (Western BlotPositive WB detected in: Rat stomach tissue, Mouse kidney tissueAll lanes: FILIP1L antibody at 2.5ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 131,103,30,100,80 KDaObserved band size: 131 KDa)

Rabbit anti-Human FILIP1L Polyclonal Antibody | anti-FILIP1L antibody

FILIP1L Antibody

Gene Names
FILIP1L; DOC1; DOC-1; GIP90; GIP130
Reactivity
Human
Applications
ELISA, Immunohistochemistry
Purity
>95%, Protein G Purified
Synonyms
FILIP1L; Polyclonal Antibody; FILIP1L Antibody; Filamin A-interacting protein 1-like; COL4A3BPIP; DOC1; GIP90; 130 kDa GPBP-interacting protein; 90 kDa GPBP-interacting protein; Protein down-regulated in ovarian cancer 1; DOC-1; anti-FILIP1L antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
>95%, Protein G Purified
Sequence Length
1133
Applicable Applications for anti-FILIP1L antibody
ELISA (EIA), Immunohistochemistry (IHC)
Storage Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Conjugate
Non-conjugated
Immunogen
Recombinant human Filamin A-interacting protein 1-like protein
Preparation and Storage
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.

Western Blot (WB)

(Western BlotPositive WB detected in: Rat stomach tissue, Mouse kidney tissueAll lanes: FILIP1L antibody at 2.5ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 131,103,30,100,80 KDaObserved band size: 131 KDa)

Western Blot (WB) (Western BlotPositive WB detected in: Rat stomach tissue, Mouse kidney tissueAll lanes: FILIP1L antibody at 2.5ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 131,103,30,100,80 KDaObserved band size: 131 KDa)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human colon cancer using MBS1499738 at dilution of 1:100)

Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human colon cancer using MBS1499738 at dilution of 1:100)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human adrenal gland tissue using MBS1499738 at dilution of 1:100)

Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human adrenal gland tissue using MBS1499738 at dilution of 1:100)

Immunofluorescence (IF)

(Immunofluorescence staining of HepG2 cells with MBS1499738 at 1:166,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).)

Immunofluorescence (IF) (Immunofluorescence staining of HepG2 cells with MBS1499738 at 1:166,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).)
Related Product Information for anti-FILIP1L antibody
Acts as a regulator of the antiangiogenic activity on endothelial cells. When overexpressed in endothelial cells, leads to inhibition of cell proliferation and migration and an increase in apoptosis. Inhibits melanoma growth When expressed in tumor-associated vasculature.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
102,441 Da
NCBI Official Full Name
filamin A-interacting protein 1-like isoform 3
NCBI Official Synonym Full Names
filamin A interacting protein 1-like
NCBI Official Symbol
FILIP1L
NCBI Official Synonym Symbols
DOC1; DOC-1; GIP90; GIP130
NCBI Protein Information
filamin A-interacting protein 1-like
UniProt Protein Name
Filamin A-interacting protein 1-like
UniProt Gene Name
FILIP1L
UniProt Synonym Gene Names
COL4A3BPIP; DOC1; GIP90; DOC-1
UniProt Entry Name
FIL1L_HUMAN

Uniprot Description

FILIP1L: Acts as a regulator of the antiangiogenic activity on endothelial cells. When overexpressed in endothelial cells, leads to inhibition of cell proliferation and migration and an increase in apoptosis. Inhibits melanoma growth When expressed in tumor- associated vasculature. Belongs to the FILIP1 family. 7 isoforms of the human protein are produced by alternative splicing.

Protein type: Unknown function

Chromosomal Location of Human Ortholog: 3q12.1

Cellular Component: cytoplasm; membrane; nucleus

Research Articles on FILIP1L

Similar Products

Product Notes

The FILIP1L filip1l (Catalog #AAA1499738) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The FILIP1L Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's FILIP1L can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunohistochemistry (IHC). Researchers should empirically determine the suitability of the FILIP1L filip1l for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "FILIP1L, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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