Rabbit anti-Human FIG4 Polyclonal Antibody | anti-FIG4 antibody

FIG4 antibody

Cat. Num. AAA9413004

• Gene Names: FIG4; YVS; BTOP; SAC3; ALS11; CMT4J; KIAA0274; dJ249I4.1
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry
• Purity: Purified by antigen-affinity chromatography.
• Synonyms: FIG4; Polyclonal Antibody; FIG4 antibody; anti-FIG4 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Purity/Purification: Purified by antigen-affinity chromatography.
• Form/Format: Supplied in 0.1M Tris-buffered saline with 10% Glycerol (pH7.0). 0.01% Thimerosal was added as a preservative.
• Sequence Length: 907

• Applicable Applications for anti-FIG4 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Western blotting: 1:500-1:3000; Immunohistochemistry: 1:50-1:500
• Immunogen Type: Recombinant protein
• Immunogen Description: Recombinant protein fragment contain a sequence corresponding to a region within amino acids 135 and 400 of FIG4
• Target Name: FIG4
• Preparation and Storage: Store at -20 degree C for long term preservation (recommended). Store at 4 degree C for short term use.

Testing Data

(Sample (30 ug of whole cell lysate) A: HCT116 7.5% SDS PAGE Primary antibody diluted at 1: 1000)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded Breast ca, using FIG4 antibody at 1: 500 dilution.)

Related Product Information for anti-FIG4 antibody

The protein encoded by this gene belongs to the SAC domain-containing protein gene family. The SAC domain, approximately 400 amino acids in length and consisting of seven conserved motifs, has been shown to possess phosphoinositide phosphatase activity. The yeast homolog, Sac1p, is involved in the regulation of various phosphoinositides, and affects diverse cellular functions such as actin cytoskeleton organization, Golgi function, and maintenance of vacuole morphology. Membrane-bound phosphoinositides function as signaling molecules and play a key role in vesicle trafficking in eukaryotic cells. Mutations in this gene have been associated with Charcot-Marie-Tooth disease, type 4J. [provided by RefSeq]

Product Categories/Family for anti-FIG4 antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 7662034
• NCBI GeneID: 9896
• NCBI Accession #: NP_055660
• NCBI GenBank Nucleotide #: NM_014845.5
• Molecular Weight: 103,635 Da
• NCBI Official Full Name: polyphosphoinositide phosphatase
• NCBI Official Synonym Full Names: FIG4 phosphoinositide 5-phosphatase
• NCBI Official Symbol: FIG4
• NCBI Official Synonym Symbols: YVS; BTOP; SAC3; ALS11; CMT4J; KIAA0274; dJ249I4.1
• NCBI Protein Information: polyphosphoinositide phosphatase
• UniProt Protein Name: Polyphosphoinositide phosphatase
• Protein Family: Polyphosphoinositide phosphatase
• UniProt Gene Name: FIG4
• UniProt Synonym Gene Names: KIAA0274; SAC3
• UniProt Entry Name: FIG4_HUMAN

NCBI Description

The protein encoded by this gene belongs to the SAC domain-containing protein gene family. The SAC domain, approximately 400 amino acids in length and consisting of seven conserved motifs, has been shown to possess phosphoinositide phosphatase activity. The yeast homolog, Sac1p, is involved in the regulation of various phosphoinositides, and affects diverse cellular functions such as actin cytoskeleton organization, Golgi function, and maintenance of vacuole morphology. Membrane-bound phosphoinositides function as signaling molecules and play a key role in vesicle trafficking in eukaryotic cells. Mutations in this gene have been associated with Charcot-Marie-Tooth disease, type 4J. [provided by RefSeq, Jul 2008]

Uniprot Description

SAC3: The PI(3,5)P2 regulatory complex regulates both the synthesis and turnover of phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2). In vitro, hydrolyzes all three D5-phosphorylated polyphosphoinositide substrates in the order PtdIns(4,5)P2 > PtdIns(3,5)P2 > PtdIns(3,4,5)P3. Plays a role in the biogenesis of endosome carrier vesicles (ECV) / multivesicular bodies (MVB) transport intermediates from early endosomes. Defects in FIG4 are the cause of Charcot-Marie-Tooth disease type 4J (CMT4J). CMT4J is a recessive demyelinating, severe form of Charcot-Marie-Tooth disease, the most common inherited disorder of the peripheral nervous system. Charcot-Marie-Tooth disease is classified in two main groups on the basis of electrophysiologic properties and histopathology: primary peripheral demyelinating neuropathies characterized by severely reduced motor nerve conduction velocities (NCVs) (less than 38m/s) and segmental demyelination and remyelination, and primary peripheral axonal neuropathies characterized by normal or mildly reduced NCVs and chronic axonal degeneration and regeneration on nerve biopsy. Defects in FIG4 are the cause of amyotrophic lateral sclerosis type 11 (ALS11). ALS is a neurodegenerative disorder affecting upper motor neurons in the brain and lower motor neurons in the brain stem and spinal cord, resulting in fatal paralysis. Sensory abnormalities are absent. Death usually occurs within 2 to 5 years. The etiology of amyotrophic lateral sclerosis is likely to be multifactorial, involving both genetic and environmental factors. The disease is inherited in 5-10%.

Protein type: EC 3.1.3.-

Chromosomal Location of Human Ortholog: 6q21

Cellular Component: Golgi membrane; recycling endosome; early endosome membrane; endoplasmic reticulum; late endosome membrane; endosome membrane

Molecular Function: phosphatidylinositol-3-phosphatase activity; protein binding; phosphatidylinositol-4-phosphate phosphatase activity; phosphatidylinositol-3,5-bisphosphate 5-phosphatase activity

Biological Process: myelin formation; dephosphorylation; pigmentation; negative regulation of myelination; phospholipid metabolic process; phosphatidylinositol biosynthetic process; neuron development; locomotory behavior; vacuole organization and biogenesis

Disease: Charcot-marie-tooth Disease, Type 4j; Yunis-varon Syndrome; Amyotrophic Lateral Sclerosis 11; Polymicrogyria, Bilateral Temporooccipital