Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded mammary cancer sections staining FGF2 in cytoplasm DAB chromogenic reaction.)
Rabbit Fibroblast Growth Factor, Basic Polyclonal Antibody
Fibroblast Growth Factor, Basic (FGFb, BFGF, Fibroblast Growth Factor 2, FGF2, Heparin-binding Growth Factor 2, HBGF-2)
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunoprecipitation, Immunohistochemistry
Purity
Affinity PurifiedPurified by immunoaffinity chromatography.
Synonyms
Fibroblast Growth Factor; Basic; Polyclonal Antibody; Basic (FGFb; BFGF; Fibroblast Growth Factor 2; FGF2; Heparin-binding Growth Factor 2; HBGF-2); Anti -Fibroblast Growth Factor; anti-Fibroblast Growth Factor; Basic antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human FGF2. Species Cross-Reactivity: mouse, rat.
Purity/Purification
Affinity Purified
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Form/Format
Supplied as a lyophilized powder from PBS, pH 7.4, 5% BSA, 0.05% thimerosal, 0.05% sodium azide. Reconstitute with sterile dH2O.
Applicable Applications for anti-Fibroblast Growth Factor, Basic antibody
Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC)
Application Notes
Suitable for use in Western Blot, Immunoprecipitation, and Immunohistochemistry.
Dilution: Western Blot:1-2ug/ml
Immunohistochemistry (paraffin):1-2ug/ml
Dilution: Western Blot:1-2ug/ml
Immunohistochemistry (paraffin):1-2ug/ml
Immunogen
Peptide mapping near the N-terminal of human FGF2, identical to the related rat and mouse sequence.
Preparation and Storage
Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded mammary cancer sections staining FGF2 in cytoplasm DAB chromogenic reaction.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded mammary cancer sections staining FGF2 in cytoplasm DAB chromogenic reaction.)
Western Blot (WB)
(Western Blot analysis of rat lung tissue lysis using FGF2 antibody.)
Western Blot (WB)
(Western Blot analysis of rat lung tissue lysis using FGF2 antibody.)
Related Product Information for anti-Fibroblast Growth Factor, Basic antibody
FGF2 has been implicated in a multitude of physiologic and pathologic processes, including limb development, angiogenesis, wound healing, and tumor growth. FGF2 belongs to the fibroblast growth factor (FGF) family. Fibroblast growth factors (FGFs) exhibit widespread mitogenic and neurotrophic activities. Nine members of the family are currently known, and FGF-1 and FGF-2 are present in relatively high levels in CNS. FGF-2 is expressed by at low levels in many tissues and cell types and reaches high concentrations in brain and pituitary.
Product Categories/Family for anti-Fibroblast Growth Factor, Basic antibody
Similar Products
Product Notes
The Fibroblast Growth Factor, Basic (Catalog #AAA624906) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Fibroblast Growth Factor, Basic (FGFb, BFGF, Fibroblast Growth Factor 2, FGF2, Heparin-binding Growth Factor 2, HBGF-2) reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Fibroblast Growth Factor, Basic can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC). Suitable for use in Western Blot, Immunoprecipitation, and Immunohistochemistry. Dilution: Western Blot:1-2ug/ml Immunohistochemistry (paraffin):1-2ug/ml. Researchers should empirically determine the suitability of the Fibroblast Growth Factor, Basic for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Fibroblast Growth Factor, Basic, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
