Rabbit anti-Human Fibrillarin Polyclonal Antibody | anti-FLRN antibody

Fibrillarin, ID (FBL, 34kD Nucleolar Scleroderma Antigen, rRNA 2'-O-methyltransferase fibrillarin, FLRN, FIB1, Nop1p, RNU3IP1) (PE)

Cat. Num. AAA6476120

• Gene Names: FBL; FIB; FLRN; Nop1; RNU3IP1
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: Fibrillarin; Polyclonal Antibody; ID (FBL; 34kD Nucleolar Scleroderma Antigen; rRNA 2'-O-methyltransferase fibrillarin; FLRN; FIB1; Nop1p; RNU3IP1) (PE); anti-FLRN antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human FBL.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-FLRN antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:50-1:100; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the center region of human FBL (KLH).
• Conjugate: PE
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of anti-FBL Antibody (Center E120) in K562 cell line lysates (35ug/lane). FBL (arrow) was detected using the purified Pab.)

Product Categories/Family for anti-FLRN antibody

Antibodies; Enzymes; Methyltransferase

References

1. Amin, M.A., Biochem. Biophys. Res. Commun. 360(2), 320-326 (2007). 2. Dunphy, J.L., Traffic 8(6), 661-672 (2007).

NCBI and Uniprot Product Information

• NCBI GI #: 12056465
• NCBI GeneID: 2091
• NCBI Accession #: NP_001427
• NCBI GenBank Nucleotide #: NM_001436.3
• UniProt Accession #: P22087; O75259; Q6IAT5; Q9UPI6; B5BUE8
• Molecular Weight: 28.9 kDa (262aa), confirmed by MALDI-TOF
• NCBI Official Full Name: rRNA 2'-O-methyltransferase fibrillarin
• NCBI Official Synonym Full Names: fibrillarin
• NCBI Official Symbol: FBL
• NCBI Official Synonym Symbols: FIB; FLRN; Nop1; RNU3IP1
• NCBI Protein Information: rRNA 2'-O-methyltransferase fibrillarin
• UniProt Protein Name: rRNA 2'-O-methyltransferase fibrillarin
• Protein Family: Fibrillarin-like rRNA/tRNA 2'-O-methyltransferase
• UniProt Gene Name: FBL
• UniProt Synonym Gene Names: FIB1; FLRN
• UniProt Entry Name: FBRL_HUMAN

NCBI Description

This gene product is a component of a nucleolar small nuclear ribonucleoprotein (snRNP) particle thought to participate in the first step in processing preribosomal RNA. It is associated with the U3, U8, and U13 small nuclear RNAs and is located in the dense fibrillar component (DFC) of the nucleolus. The encoded protein contains an N-terminal repetitive domain that is rich in glycine and arginine residues, like fibrillarins in other species. Its central region resembles an RNA-binding domain and contains an RNP consensus sequence. Antisera from approximately 8% of humans with the autoimmune disease scleroderma recognize fibrillarin. [provided by RefSeq, Jul 2008]

Uniprot Description

FBL: Involved in pre-rRNA processing. Utilizes the methyl donor S-adenosyl-L-methionine to catalyze the site-specific 2'- hydroxyl methylation of ribose moieties in pre-ribosomal RNA. Site specificity is provided by a guide RNA that base pairs with the substrate. Methylation occurs at a characteristic distance from the sequence involved in base pairing with the guide RNA. Interacts with NOLC1. Component of box C/D small nucleolar ribonucleoprotein (snoRNP) particles that contain NHP2L1, FBL, NOP5 and NOP56, plus a guide RNA. It is associated with the U3, U8, U13, X and Y small nuclear RNAs. Component of several ribosomal and nucleolar protein complexes. Interacts with PRMT5 and UTP20. Interacts with DDX5. Belongs to the methyltransferase superfamily. Fibrillarin family.

Protein type: RNA-binding; RNA processing; Methyltransferase; Nucleolus; EC 2.1.1.-

Chromosomal Location of Human Ortholog: 19q13.1

Cellular Component: Cajal body; granular component; membrane; nucleolus; nucleus

Molecular Function: protein binding; RNA binding

Biological Process: osteoblast differentiation; snoRNA metabolic process; tRNA processing; rRNA processing