Rabbit anti-Human, Mouse FHL3 Polyclonal Antibody | anti-FHL3 antibody

FHL3 Rabbit pAb

Cat. Num. AAA4757963

• Gene Names: FHL3; SLIM2
• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity purified
• Synonyms: FHL3; Polyclonal Antibody; FHL3 Rabbit pAb; four and a half LIM domains 3; anti-FHL3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Purity/Purification: Affinity purified
• Form/Format: pH7.4 PBS, 0.05% NaN3, 40% Glycerol

• Applicable Applications for anti-FHL3 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)
• Application Notes: WB: 1:1000-1:5000; ELISA: 1:2000-1:10,000; IHC: 1:50-1:200
• Immunogen: Fusion protein of human FHL3
• Conjugation: Unconjugated
• Modification: Unmodified
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Gel: 8%SDS-PAGE, Lysate: 40 ug, Lane: Mouse skeletal muscle tissue, Primary antibody: 822372(FHL3 Antibody) at dilution 1/1600, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 30 seconds)

Immunohistochemistry (IHC)

(The image is immunohistochemistry of paraffin-embedded Human cervical cancer tissue using 822372(FHL3 Antibody) at dilution 1/40. (Original magnification: ×200))

Immunohistochemistry (IHC)

(The image is immunohistochemistry of paraffin-embedded Human brain tissue using 822372(FHL3 Antibody) at dilution 1/40. (Original magnification: ×200))

Related Product Information for anti-FHL3 antibody

The protein encoded by this gene is a member of a family of proteins containing a four-and-a-half LIM domain, which is a highly conserved double zinc finger motif. The encoded protein has been shown to interact with the cancer developmental regulators SMAD2, SMAD3, and SMAD4, the skeletal muscle myogenesis protein MyoD, and the high-affinity IgE beta chain regulator MZF-1. This protein may be involved in tumor suppression, repression of MyoD expression, and repression of IgE receptor expression. Two transcript variants encoding different isoforms have been found for this gene.

Product Categories/Family for anti-FHL3 antibody

Primary antibody

NCBI and Uniprot Product Information

• NCBI GI #: 345197222
• NCBI GeneID: 2275
• NCBI Accession #: NP_001230807.1
• NCBI GenBank Nucleotide #: NM_001243878.1
• UniProt Accession #: Q13643; Q6I9T0; Q9BVA2; D3DPT6
• Molecular Weight: 31,192 Da
• NCBI Official Full Name: four and a half LIM domains protein 3 isoform 2
• NCBI Official Synonym Full Names: four and a half LIM domains 3
• NCBI Official Symbol: FHL3
• NCBI Official Synonym Symbols: SLIM2
• NCBI Protein Information: four and a half LIM domains protein 3; FHL-3; LIM-only protein FHL3; SLIM-2; skeletal muscle LIM-protein 2
• UniProt Protein Name: Four and a half LIM domains protein 3
• Protein Family: Four and a half LIM domains protein
• UniProt Gene Name: FHL3
• UniProt Synonym Gene Names: SLIM2; FHL-3; SLIM-2
• UniProt Entry Name: FHL3_HUMAN

NCBI Description

The protein encoded by this gene is a member of a family of proteins containing a four-and-a-half LIM domain, which is a highly conserved double zinc finger motif. The encoded protein has been shown to interact with the cancer developmental regulators SMAD2, SMAD3, and SMAD4, the skeletal muscle myogenesis protein MyoD, and the high-affinity IgE beta chain regulator MZF-1. This protein may be involved in tumor suppression, repression of MyoD expression, and repression of IgE receptor expression. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2011]

Uniprot Description

FHL3:

Protein type: Actin-binding; Contractile

Chromosomal Location of Human Ortholog: 1p34

Cellular Component: focal adhesion; stress fiber; nucleus; Z disc

Molecular Function: zinc ion binding; actin binding

Biological Process: muscle development; actin cytoskeleton organization and biogenesis