Canine Brain Natriuretic Peptide (BNP) ELISA Kit | BNP elisa kit

Canine Brain Natriuretic Peptide (BNP) ELISA Kit

Cat. Num. AAA2019551

• Gene Names: NPPB; BNP
• Reactivity: Canine
• Synonyms: Brain Natriuretic Peptide (BNP); Canine Brain Natriuretic Peptide (BNP) ELISA Kit; GC-B; NPPB; B-Type Natriuretic Peptide; Natriuretic Peptide Precursor B; Gamma-brain natriuretic peptide; Brain natriuretic peptide 32; BNP elisa kit

• Reactivity: Canine
• Specificity: This assay has high sensitivity and excellent specificity for detection of BNP. No significant cross-reactivity or interference between BNP and analogues was observed.
• Sequence Length: 1

• Samples: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Assay Type: Quantitative Competitive
• Detection Range: 24.69-2,000pg/mL
• Sensitivity: < 9.39pg/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level BNP were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level BNP were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

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Related Product Information for BNP elisa kit

Intended Uses: The kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of BNP in canine serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to BNP has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled BNP and unlabeled BNP (Standards or samples) with the pre-coated antibody specific to BNP. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of BNP in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of BNP in the sample.

Product Categories/Family for BNP elisa kit

Cardiovascular biology; Neuroscience; Hormone metabolism

NCBI and Uniprot Product Information

• NCBI GI #: 4433127
• NCBI GeneID: 4879
• Molecular Weight: 14,726 Da
• NCBI Official Full Name: brain natriuretic peptide, partial
• NCBI Official Synonym Full Names: natriuretic peptide B
• NCBI Official Symbol: NPPB
• NCBI Official Synonym Symbols: BNP
• NCBI Protein Information: natriuretic peptides B
• UniProt Protein Name: Natriuretic peptides B
• UniProt Gene Name: NPPB
• UniProt Synonym Gene Names: BNP(1-32); BNP-32
• UniProt Entry Name: ANFB_HUMAN

NCBI Description

This gene is a member of the natriuretic peptide family and encodes a secreted protein which functions as a cardiac hormone. The protein undergoes two cleavage events, one within the cell and a second after secretion into the blood. The protein's biological actions include natriuresis, diuresis, vasorelaxation, inhibition of renin and aldosterone secretion, and a key role in cardiovascular homeostasis. A high concentration of this protein in the bloodstream is indicative of heart failure. The protein also acts as an antimicrobial peptide with antibacterial and antifungal activity. Mutations in this gene have been associated with postmenopausal osteoporosis. [provided by RefSeq, Nov 2014]

Uniprot Description

NPPB: Cardiac hormone which may function as a paracrine antifibrotic factor in the heart. Also plays a key role in cardiovascular homeostasis through natriuresis, diuresis, vasorelaxation, and inhibition of renin and aldosterone secretion. Specifically binds and stimulates the cGMP production of the NPR1 receptor. Binds the clearance receptor NPR3. Belongs to the natriuretic peptide family.

Protein type: Secreted, signal peptide; Secreted; Hormone

Chromosomal Location of Human Ortholog: 1p36.2

Cellular Component: extracellular space; protein complex; extracellular region

Molecular Function: diuretic hormone activity; hormone activity; receptor binding

Biological Process: cGMP biosynthetic process; negative regulation of angiogenesis; cell surface receptor linked signal transduction; regulation of vasodilation; receptor guanylyl cyclase signaling pathway; regulation of blood pressure; negative regulation of cell growth; regulation of vascular permeability; body fluid secretion; regulation of blood vessel size