Western Blot (WB)
(FGD2 Antibody (C-term) western blot analysis in mouse stomach tissue lysates (35ug/lane).This demonstrates the FGD2 antibody detected the FGD2 protein (arrow).)
Rabbit anti-Mouse FGD2 Polyclonal Antibody | anti-Fgd2 antibody
FGD2, CT (FGD2, ZFYVE4, FYVE, RhoGEF and PH domain-containing protein 2, Zinc finger FYVE domain-containing protein 4)
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Immunohistochemistry: 1:10-50
Western Blot (WB)
(FGD2 Antibody (C-term) western blot analysis in mouse stomach tissue lysates (35ug/lane).This demonstrates the FGD2 antibody detected the FGD2 protein (arrow).)
Western Blot (WB)
(FGD2 Antibody (C-term) western blot analysis in mouse stomach tissue lysates (35ug/lane).This demonstrates the FGD2 antibody detected the FGD2 protein (arrow).)
Immunohistochemistry (IHC)
(FGD2 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of FGD2 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(FGD2 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of FGD2 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
NCBI and Uniprot Product Information
Uniprot Description
Function: Activates CDC42, a member of the Ras-like family of Rho- and Rac proteins, by exchanging bound GDP for free GTP. Activates JNK1 via CDC42 but not RAC1. Binds to phosphatidylinositol 4,5-bisphosphate, phosphatidylinositol 3,4,5-trisphosphate, phosphatidylinositol 5-monophosphate, phosphatidylinositol 4-monophosphate and phosphatidylinositol 3-monophosphate. Ref.2
Subcellular location: Cytoplasm. Nucleus. Early endosome. Early endosome membrane. Cell projection › ruffle membrane. Cytoplasm › cytoskeleton
Probable. Note: Recruitment to the endosome and ruffle membrane requires the presence of phosphoinositides. Ref.2
Tissue specificity: Lymph node, spleen, B-lymphocytes and macrophages (at protein level). Expressed at high levels in lymph node, spleen, B-lymphocytes and bone marrow macrophages. Expressed at lower levels in mature bone marrow dendritic cells. In both immature and mature B-cells, expression is down-regulated by prior B-cell receptor signaling. Expression remains high in resting B and memory cells but declines upon differentiation into plasma cells. Ref.1 Ref.2
Domain: The FYVE-type zinc-finger is necessary for early endosome localization. Recruitment to endosomal membranes via this domain requires the presence of phosphatidylinositol 3-phosphate or other phosphatidylinositides. Ref.2The PH domain is necessary for localization to the ruffle membrane. Recruitment to ruffle membrane occurs through binding of phosphoinositides by the PH domain. This domain also contributes to the lipid-binding properties of the protein. Ref.2The DH domain is necessary for its ability to activate JNK1 via CDC42. Ref.2
Sequence similarities: Contains 1 DH (DBL-homology) domain.Contains 1 FYVE-type zinc finger.Contains 2 PH domains.
Sequence caution: The sequence AAC35430.1 differs from that shown. Reason: Frameshift at position 606. The sequence AAP45200.1 differs from that shown. Reason: Erroneous gene model prediction.
Research Articles on Fgd2
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Product Notes
The Fgd2 fgd2 (Catalog #AAA648315) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The FGD2, CT (FGD2, ZFYVE4, FYVE, RhoGEF and PH domain-containing protein 2, Zinc finger FYVE domain-containing protein 4) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's FGD2 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC). Suitable for use in Western Blot, Immunohistochemistry, ELISA Dilution: ELISA: 1:1,000 Western Blot: 1:100-500 Immunohistochemistry: 1:10-50. Researchers should empirically determine the suitability of the Fgd2 fgd2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "FGD2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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