Rabbit Ferritin Light Chain Polyclonal Antibody | anti-FTL antibody

Ferritin Light Chain Polyclonal Antibody

Cat. Num. AAA3012686

• Gene Names: FTL; LFTD; NBIA3
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunoprecipitation, Immunocytochemistry
• Purity: ProA affinity purified
• Synonyms: Ferritin Light Chain; Polyclonal Antibody; Ferritin Light Chain Polyclonal Antibody; Ferritin L subunit; FTL; Ferritin L chain; Ferritin light chain; Ferritin light polypeptide; ferritin light polypeptide like 3; FRIL; LFTD; NBIA 3; NBIA3; anti-FTL antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Ferritin Light Chain polyclonal antibody detects endogenous levels of Ferritin Light Chain protein.
• Purity/Purification: ProA affinity purified
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 175

• Applicable Applications for anti-FTL antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)
• Application Notes: WB: 1:1,000-1:5,000; IHC: 1:100-1:500; ICC: 1:100-1:200
• Immunogen: Recombinant protein
• Preparation and Storage: Store at 4 degree C after thawing.; Aliquot store at -20 degree C or -80 degree C.; Avoid repeated freeze/thaw cycles.

Western Blot (WB)

(Western Blot analysis of Ferritin Light Chain on rat liver tissue lysate using anti-Ferritin Light Chain antibody at 1/1,000 dilution.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Ferritin Light Chain antibody. Counter stained with hematoxylin.)

Related Product Information for anti-FTL antibody

Mammalian ferritins consist of 24 subunits made up of two types of polypeptide chains, ferritin heavy chain and ferritin light chain, which each have unique functions. Ferritin heavy chains catalyze the first step in iron storage, the oxidation of Fe (II), whereas ferritin light chains promote the nucleation of ferrihydrite, enabling storage of Fe (III). The most prominent role of mammalian ferritins is to provide iron-buffering capacity to cells. In addition to iron buffering, heavy chain ferritin is also involved in the regulation of thymidine biosynthesis via increased expression of cytoplasmic serine hydroxymethyltransferase, which is a limiting factor in thymidylate synthesis in MCF-7 cells. Light chain ferritin is involved in cataracts by at least two mechanisms, hereditary hyperferritinemia cataract syndrome, in which light chain ferritin is overexpressed, and oxidative stress, an important factor in the development of ageing-related cataracts. The gene encoding human ferritin heavy chain maps to chromosome 11q13 and the human ferritin light chain gene maps to chromosome 19q13.3-q13.4.

NCBI and Uniprot Product Information

• NCBI GI #: 20149498
• NCBI GeneID: 2512
• NCBI Accession #: NP_000137.2
• NCBI GenBank Nucleotide #: NP_000137.2
• UniProt Accession #: P02792; P29391; P02793
• Molecular Weight: 54kDa
• NCBI Official Full Name: ferritin light chain
• NCBI Official Synonym Full Names: ferritin light chain
• NCBI Official Symbol: FTL
• NCBI Official Synonym Symbols: LFTD; NBIA3
• NCBI Protein Information: ferritin light chain
• UniProt Protein Name: Ferritin light chain
• Protein Family: Ferritin
• UniProt Gene Name: FTL
• UniProt Synonym Gene Names: Ferritin L subunit
• UniProt Entry Name: FRIL_HUMAN

NCBI Description

This gene encodes the light subunit of the ferritin protein. Ferritin is the major intracellular iron storage protein in prokaryotes and eukaryotes. It is composed of 24 subunits of the heavy and light ferritin chains. Variation in ferritin subunit composition may affect the rates of iron uptake and release in different tissues. A major function of ferritin is the storage of iron in a soluble and nontoxic state. Defects in this light chain ferritin gene are associated with several neurodegenerative diseases and hyperferritinemia-cataract syndrome. This gene has multiple pseudogenes. [provided by RefSeq, Jul 2008]

Uniprot Description

FTL: Stores iron in a soluble, non-toxic, readily available form. Important for iron homeostasis. Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation. Also plays a role in delivery of iron to cells. Mediates iron uptake in capsule cells of the developing kidney. Defects in FTL are the cause of hereditary hyperferritinemia-cataract syndrome (HHCS). It is an autosomal dominant disease characterized by early-onset bilateral cataract. Affected patients have elevated level of circulating ferritin. HHCS is caused by mutations in the iron responsive element (IRE) of the FTL gene. Defects in FTL are the cause of neurodegeneration with brain iron accumulation type 3 (NBIA3); also known as adult-onset basal ganglia disease. It is a movement disorder with heterogeneous presentations starting in the fourth to sixth decade. It is characterized by a variety of neurological signs including parkinsonism, ataxia, corticospinal signs, mild nonprogressive cognitive deficit and episodic psychosis. It is linked with decreased serum ferritin levels. Belongs to the ferritin family.

Protein type: Oxidoreductase

Chromosomal Location of Human Ortholog: 19q13.33

Cellular Component: ferritin complex; membrane; cytosol

Molecular Function: identical protein binding; protein binding; ferric iron binding; iron ion binding

Biological Process: receptor-mediated endocytosis; iron ion homeostasis; cellular iron ion homeostasis; post-Golgi vesicle-mediated transport; transmembrane transport; iron ion transport

Disease: Hyperferritinemia With Or Without Cataract; L-ferritin Deficiency; Neurodegeneration With Brain Iron Accumulation 3