Rabbit anti-Human Ferritin H-chain Polyclonal Antibody | anti-FTH1 antibody

Ferritin H-chain (FTH)

Cat. Num. AAA615198

• Gene Names: FTH1; FHC; FTH; PLIF; FTHL6; PIG15; MGC104426
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Serum; Serum
• Synonyms: Ferritin H-chain; Polyclonal Antibody; Ferritin H-chain (FTH); Anti -Ferritin H-chain (FTH); anti-FTH1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes Human FTH heavy chain. No sequence homology with ferritin L-chain. Sequnce Homology
• Purity/Purification: Serum; Serum
• Form/Format: Supplied as a liquid in PBS, 0.05% sodium azide.

• Applicable Applications for anti-FTH1 antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Dilution: Western Blot: 1:1000-1:5000 using ECL.; ELISA: 10,000-50,000. Control peptide can be used to coat plates at 1ug/ml
• Immunogen: Synthetic peptide (15aa) near the NT of human/mouse/rat FTH (KLH).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Related Product Information for anti-FTH1 antibody

Elemental iron is required for a variety of normal cellular functions. It is vital for proper growth and development. Ferritin is the major protein involved in iron sequestration and detoxification. Ferritin is found in all living species. Mammalian liver and spleen ferritin (~450kD) consists of 24 subunits of 2 species, the heavy subunit (~21kD; FTH) and the light subunit (~19kD; FTL). The 2 types of apoferritin subunits were designated H and L for heart and liver, respectively. Ferritin molecules from plants and bacteria contain only H-type chains. 'H-type' is associated with the presence of centers catalyzing the oxidation of two Fe(II) atoms. FTL subunit is coded by a gene in segment 19q13.3. FTH subunit is located on chromosome 11. Ferritin is capable of storing up to 4500 atoms of ferric iron. The H-to-L ratio within ferritin varies in a tissue-specific manner. This ratio is also influenced by pathophysiological conditions, including inflammation and malignancy. Hyperferritinemia-cataract syndrome has a mutation in the iron response element (IRE) in the 5-prime noncoding region of the FTL gene. Synthesis of both ferritin subunits is controlled by a common cytosolic protein, iron regulatory proteins (IRPs), which binds to the iron-responsive element (IRE) in the 5'-UTR of the H- and L-ferritin mRNAs. H-chains are important for Fe(II) oxidation. L-chains assist in core formation.

Product Categories/Family for anti-FTH1 antibody

Antibodies; Abs to Serum Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 306744
• NCBI GeneID: 2495
• UniProt Accession #: P02794; Q3KRA8; Q3SWW1; B3KNR5; Q6NS36; Q6NZ44; Q8TD27
• Molecular Weight: 21,226 Da
• NCBI Official Full Name: ferritin
• NCBI Official Synonym Full Names: ferritin, heavy polypeptide 1
• NCBI Official Symbol: FTH1
• NCBI Official Synonym Symbols: FHC; FTH; PLIF; FTHL6; PIG15; MGC104426
• NCBI Protein Information: ferritin heavy chain; apoferritin; OTTHUMP00000232197; OTTHUMP00000232244; OTTHUMP00000232245; ferritin H subunit; placenta immunoregulatory factor; proliferation-inducing protein 15; cell proliferation-inducing gene 15 protein
• UniProt Protein Name: Ferritin heavy chain
• Protein Family: Iron transporter
• UniProt Gene Name: FTH1
• UniProt Synonym Gene Names: FTH; FTHL6
• UniProt Entry Name: FRIH_HUMAN

NCBI Description

This gene encodes the heavy subunit of ferritin, the major intracellular iron storage protein in prokaryotes and eukaryotes. It is composed of 24 subunits of the heavy and light ferritin chains. Variation in ferritin subunit composition may affect the rates of iron uptake and release in different tissues. A major function of ferritin is the storage of iron in a soluble and nontoxic state. Defects in ferritin proteins are associated with several neurodegenerative diseases. This gene has multiple pseudogenes. Several alternatively spliced transcript variants have been observed, but their biological validity has not been determined. [provided by RefSeq]

Uniprot Description

Ferritin-H: Stores iron in a soluble, non-toxic, readily available form. Important for iron homeostasis. Has ferroxidase activity. Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation. Also plays a role in delivery of iron to cells. Mediates iron uptake in capsule cells of the developing kidney. Oligomer of 24 subunits. There are two types of subunits: L (light) chain and H (heavy) chain. The major chain can be light or heavy, depending on the species and tissue type. The functional molecule forms a roughly spherical shell with a diameter of 12 nm and contains a central cavity into which the insoluble mineral iron core is deposited. Belongs to the ferritin family.

Protein type: Oxidoreductase; EC 1.16.3.1; Cofactor and Vitamin Metabolism - porphyrin and chlorophyll

Chromosomal Location of Human Ortholog: 11q13

Cellular Component: ferritin complex; mitochondrion; cytosol; nucleus

Molecular Function: ferroxidase activity; protein binding; ferric iron binding; iron ion binding

Biological Process: receptor-mediated endocytosis; negative regulation of cell proliferation; cellular iron ion homeostasis; negative regulation of fibroblast proliferation; immune response; post-Golgi vesicle-mediated transport; transmembrane transport; iron ion transport; intracellular sequestering of iron ion

Disease: Hemochromatosis, Type 5