Rabbit anti-Human FDFT1 Polyclonal Antibody | anti-FDFT1 antibody

FDFT1, CT (FDFT1, Squalene synthase, FPP:FPP farnesyltransferase, Farnesyl-diphosphate farnesyltransferase) (Azide free) (HRP)

Cat. Num. AAA6298773

• Gene Names: FDFT1; SS; SQS; DGPT; ERG9
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: FDFT1; Polyclonal Antibody; CT (FDFT1; Squalene synthase; FPP:FPP farnesyltransferase; Farnesyl-diphosphate farnesyltransferase) (Azide free) (HRP); Farnesyl-diphosphate farnesyltransferase; anti-FDFT1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-FDFT1 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: FDFT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 331-361 amino acids from the C-terminal region of human FDFT1.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(FDFT1 Antibody (C-term) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the FDFT1 antibody detected the FDFT1 protein (arrow).)

Western Blot (WB)

(Western blot analysis of FDFT1 (arrow) using rabbit polyclonal FDFT1 Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the FDFT1 gene.)

Related Product Information for anti-FDFT1 antibody

This gene encodes a membrane-associated enzyme located at a branch point in the mevalonate pathway. The encoded protein is the first specific enzyme in cholesterol biosynthesis, catalyzing the dimerization of two molecules of farnesyl diphosphate in a two-step reaction to form squalene.

Product Categories/Family for anti-FDFT1 antibody

Antibodies; Enzymes; Synthase

References

Chalasani, N., et al. Gastroenterology 139(5):1567-1576(2010). Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010). Kovanen, L., et al. Alcohol Alcohol. 45(4):303-311(2010). Lipkin, S.M., et al. Cancer Prev Res (Phila) 3(5):597-603(2010). Sjoholm, L.K., et al. J Circadian Rhythms 8, 1 (2010).

NCBI and Uniprot Product Information

• NCBI GI #: 67089147
• NCBI GeneID: 2222
• NCBI Accession #: NP_004453.3
• NCBI GenBank Nucleotide #: NM_004462.3
• UniProt Accession #: P37268; Q96GT0
• Molecular Weight: 48,115 Da
• NCBI Official Full Name: squalene synthase
• NCBI Official Synonym Full Names: farnesyl-diphosphate farnesyltransferase 1
• NCBI Official Symbol: FDFT1
• NCBI Official Synonym Symbols: SS; SQS; DGPT; ERG9
• NCBI Protein Information: squalene synthase; squalene synthetase; FPP:FPP farnesyltransferase; presqualene-di-diphosphate synthase
• UniProt Protein Name: Squalene synthase
• UniProt Gene Name: FDFT1
• UniProt Synonym Gene Names: SQS; SS
• UniProt Entry Name: FDFT_HUMAN

Uniprot Description

FDFT1: a membrane-associated enzyme located at a branch point in the mevalonate pathway. The encoded protein is the first specific enzyme in cholesterol biosynthesis, catalyzing the dimerization of two molecules of farnesyl diphosphate in a two-step reaction to form squalene. [provided by RefSeq, Jul 2008]

Protein type: EC 2.5.1.21; Endoplasmic reticulum; Lipid Metabolism - steroid biosynthesis; Membrane protein, integral; Membrane protein, multi-pass; Oxidoreductase; Transferase

Chromosomal Location of Human Ortholog: 8p23.1-p22

Cellular Component: endoplasmic reticulum; endoplasmic reticulum membrane

Molecular Function: farnesyl-diphosphate farnesyltransferase activity

Biological Process: cholesterol biosynthetic process; farnesyl diphosphate metabolic process; steroid biosynthetic process