Rabbit anti-Human FCRL4 Polyclonal Antibody | anti-FCRL4 antibody

FCRL4, CT (FCRL4, FCRH4, IFGP2, IRTA1, Fc receptor-like protein 4, Fc receptor homolog 4, IFGP family protein 2, Immune receptor translocation-associated protein 1, CD307d) (Biotin)

Cat. Num. AAA6298738

• Gene Names: FCRL4; FCRH4; IGFP2; IRTA1; MGC150522; MGC150523
• Reactivity: Human
• Applications: Western Blot, ELISA, Flow Cytometry, Functional Assay
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: FCRL4; Polyclonal Antibody; CT (FCRL4; FCRH4; IFGP2; IRTA1; Fc receptor-like protein 4; Fc receptor homolog 4; IFGP family protein 2; Immune receptor translocation-associated protein 1; CD307d) (Biotin); CD307d; anti-FCRL4 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human FCRL4.
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-FCRL4 antibody: Western Blot (WB), ELISA (EIA), Flow Cytometry (FC/FACS)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 480-507 in the C-terminal region of human FCRL4.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of FCRL4 (arrow) was detected using 35592 (1:2000). Lane 1: Daudi whole cell lysate (20ug/lane) Lane 2: Raji whole cell lysate (20ug/lane) Secondary IgG (HRP) Goat Anti-Rabbit Predicted band size : 57kD Blocking/Dilution buffer: 5% NFDM/TBST.)

Western Blot (WB)

(Western Blot analysis of FCRL4 (arrow) was detected using 35592 (1:2000). Lane 1: 293T/17 whole cell lysate (20ug/lane) Lane 2: Ramos whole cell lysate (20ug/lane) Lane 3: Raji whole cell lysate (20ug/lane) Lane 4: Daudi whole cell lysate (20ug/lane) Secondary IgG (HRP) Goat Anti-Rabbit Predicted band size : 57kD Blocking/Dilution buffer: 5% NFDM/TBST.)

Flow Cytometry (FC/FACS)

(Flow Cytometric analysis of Ramos cells (right histogram) using MBS6000748 compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-FCRL4 antibody

This gene encodes a member of the immunoglobulin receptor superfamily and is one of several Fc receptor-like glycoproteins clustered on the long arm of chromosome 1. The encoded protein has four extracellular C2-type immunoglobulin domains, a transmembrane domain and a cytoplasmic domain that contains three immune-receptor tyrosine-based inhibitory motifs. This protein may play a role in the function of memory B-cells in the epithelia. Aberrations in the chromosomal region encoding this gene are associated with non-Hodgkin lymphoma and multiple myeloma.

Product Categories/Family for anti-FCRL4 antibody

Antibodies; CD Markers

References

1. Davila, S., et al. Genes Immun. (2010). In press: 2. Weiss, G.E., et al. J. Immunol. 183(3):2176-2182(2009). 3. Kazemi, T., et al. Cancer Immunol. Immunother. 58(6):989-996(2009). 4. Falini, B., et al. Blood 102(10):3684-3692(2003).

NCBI and Uniprot Product Information

• NCBI GI #: 14550416
• NCBI GeneID: 83417
• NCBI Accession #: NP_112572.1
• NCBI GenBank Nucleotide #: NM_031282.2
• UniProt Accession #: Q96PJ5; Q96PJ3; Q96RE0
• Molecular Weight: 57,224 Da
• NCBI Official Full Name: Fc receptor-like protein 4
• NCBI Official Synonym Full Names: Fc receptor-like 4
• NCBI Official Symbol: FCRL4
• NCBI Official Synonym Symbols: FCRH4; IGFP2; IRTA1; MGC150522; MGC150523
• NCBI Protein Information: Fc receptor-like protein 4; hIFGP2; OTTHUMP00000033529; fcR-like protein 4; IFGP family protein 2; fc receptor homolog 4; immunoglobulin superfamily Fc receptor, gp42; immune receptor translocation-associated protein 1; immunoglobulin superfamily receptor
• UniProt Protein Name: Fc receptor-like protein 4
• Protein Family: Fc receptor-like protein
• UniProt Gene Name: FCRL4
• UniProt Synonym Gene Names: FCRH4; IFGP2; IRTA1
• UniProt Entry Name: FCRL4_HUMAN

NCBI Description

This gene encodes a member of the immunoglobulin receptor superfamily and is one of several Fc receptor-like glycoproteins clustered on the long arm of chromosome 1. The encoded protein has four extracellular C2-type immunoglobulin domains, a transmembrane domain and a cytoplasmic domain that contains three immune-receptor tyrosine-based inhibitory motifs. This protein may play a role in the function of memory B-cells in the epithelia. Aberrations in the chromosomal region encoding this gene are associated with non-Hodgkin lymphoma and multiple myeloma. [provided by RefSeq]

Uniprot Description

FCRL4: May function as an inhibitor of the B-cell receptor signaling. May function in the B-cell-mediated immune response. A chromosomal aberration involving FCRL4 is found in non-Hodgkin lymphoma (NHG). Translocation t(1;1)(p36.3; q21.1-2). A chromosomal aberration involving FCRL4 is found in multiple myeloma (MM). Translocation t(1;14)(q21;q32) that forms a FCRL4-IGHA1 fusion protein. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 1q21

Cellular Component: integral to membrane; plasma membrane

Molecular Function: protein binding

Biological Process: immune system process