Western Blot (WB)
((0.5ug/ml) staining of Human Tonsil (A) and Thymus (B) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat FCRL1 Polyclonal Antibody | anti-FCRL1 antibody
Goat anti-FCRL1 Antibody
Gene Names
FCRL1; FCRH1; IFGP1; IRTA5; CD307a
Reactivity
Tested: Human; Expected from sequence similarity: Human
Applications
ELISA, Western Blot
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms
FCRL1; Polyclonal Antibody; Goat anti-FCRL1 Antibody; Goat Anti-FCRL1 (aa165-177) Antibody; Fc receptor-like 1; CD307a; FCRH1; IFGP1; IRTA5; Fc receptor-like protein 1; IFGP family protein 1; fc receptor homolog 1; fcR-like protein 1; hIFGP1; immune receptor translocation-associated protein 5; immunoglobulin superfamily Fc receptor; gp42; FCRL1 antibody; Fc receptor-like 1 antibody; CD307a antibody; FCRH1 antibody; IFGP1 antibody; IRTA5 antibody; Fc receptor-like protein 1 antibody; IFGP family protein 1 antibody; fc receptor homolog 1 antibody; fcR-like protein 1 antibody; hIFGP1 antibody; immune receptor translocation-associated protein 5 antibody; gp42 antibody; anti-FCRL1 antibody
Host
Goat
Reactivity
Tested: Human; Expected from sequence similarity: Human
Clonality
Polyclonal
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (varies by lot)
Sequence
SRLRKANITDVD
Sequence Length
429
Applicable Applications for anti-FCRL1 antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes
Peptide ELISA: Antibody detection limit dilution 1: 64000.
Western Blot: Approx 55+48kDa bands observed in Human Tonsil and Thymus lysates (calculated MW of 46.9kDa according to NP_443170.1). The observed molecular weights correspond to the glycosylated forms. Recommended concentration: 0.3-1ug/ml.
Western Blot: Approx 55+48kDa bands observed in Human Tonsil and Thymus lysates (calculated MW of 46.9kDa according to NP_443170.1). The observed molecular weights correspond to the glycosylated forms. Recommended concentration: 0.3-1ug/ml.
Immunogen
Peptide with sequence C-SRLRKANITDVD, from the C Terminus of the protein sequence according to NP_443170.1; NP_001152870.1.
Epitope
C Terminus
Note
This antibody is expected to recognize isoform 1 (NP_443170.1) and isoform 3 (NP_001152870.1).
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Western Blot (WB)
((0.5ug/ml) staining of Human Tonsil (A) and Thymus (B) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((0.5ug/ml) staining of Human Tonsil (A) and Thymus (B) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
22,068 Da
NCBI Official Full Name
Fc receptor-like protein 1 isoform 1
NCBI Official Synonym Full Names
Fc receptor like 1
NCBI Official Symbol
FCRL1
NCBI Official Synonym Symbols
FCRH1; IFGP1; IRTA5; CD307a
NCBI Protein Information
Fc receptor-like protein 1
UniProt Protein Name
Fc receptor-like protein 1
Protein Family
UniProt Gene Name
FCRL1
UniProt Synonym Gene Names
FCRH1; IFGP1; IRTA5; FcR-like protein 1; FcRL1; FcRH1; hIFGP1
UniProt Entry Name
FCRL1_HUMAN
NCBI Description
This gene encodes a member of the immunoglobulin receptor superfamily and is one of several Fc receptor-like glycoproteins clustered on the long arm of chromosome 1. The encoded protein contains three extracellular C2-like immunoglobulin domains, a transmembrane domain and a cytoplasmic domain with two immunoreceptor-tyrosine activation motifs. This protein may play a role in the regulation of cancer cell growth. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2009]
Uniprot Description
FCRL1: May function as an activating coreceptor in B-cells. May function in B-cells activation and differentiation. 4 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, integral
Chromosomal Location of Human Ortholog: 1q21-q22
Research Articles on FCRL1
Similar Products
Product Notes
The FCRL1 fcrl1 (Catalog #AAA423556) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-FCRL1 Antibody reacts with Tested: Human; Expected from sequence similarity: Human and may cross-react with other species as described in the data sheet. AAA Biotech's FCRL1 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 64000. Western Blot: Approx 55+48kDa bands observed in Human Tonsil and Thymus lysates (calculated MW of 46.9kDa according to NP_443170.1). The observed molecular weights correspond to the glycosylated forms. Recommended concentration: 0.3-1ug/ml. Researchers should empirically determine the suitability of the FCRL1 fcrl1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: SRLRKANITD VD. It is sometimes possible for the material contained within the vial of "FCRL1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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