Rabbit anti-Human FBXO44 Polyclonal Antibody | anti-FBXO44 antibody

FBXO44, NT (FBXO44, FBG3, FBX30, FBX44, FBX6A, FBXO6A, F-box only protein 44, F-box protein FBX30, F-box/G-domain protein 3) (FITC)

Cat. Num. AAA6298453

• Gene Names: FBXO44; FBG3; FBX30; FBX6A; Fbx44; Fbxo6a
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: FBXO44; Polyclonal Antibody; NT (FBXO44; FBG3; FBX30; FBX44; FBX6A; FBXO6A; F-box only protein 44; F-box protein FBX30; F-box/G-domain protein 3) (FITC); F-box/G-domain protein 3; anti-FBXO44 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-FBXO44 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: FBXO44 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 22-51 amino acids from the N-terminal region of human FBXO44.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(FBXO44 Antibody (N-term) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the FBXO44 Antibody detected the FBXO44 protein (arrow).)

Product Categories/Family for anti-FBXO44 antibody

Antibodies; Ubiquitin Ligases

References

Kumanomidou, T., et al. Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun. 66 (PT 1), 95-98 (2010): Glenn, K.A., et al. J. Biol. Chem. 283(19):12717-12729(2008). Lamesch, P., et al. Genomics 89(3):307-315(2007). Jin, J., et al. Genes Dev. 18(21):2573-2580(2004). Ilyin, G.P., et al. Gene 296 (1-2), 11-20 (2002).

NCBI and Uniprot Product Information

• NCBI GI #: 62388868
• NCBI GeneID: 93611
• NCBI Accession #: NP_001014765.1
• NCBI GenBank Nucleotide #: NM_001014765.1
• UniProt Accession #: Q9H4M3; Q5TGX2; Q5TGX4; Q5TGX5; Q68DJ9; Q8WWY2; B3KNZ2; B7Z743
• Molecular Weight: 29,747 Da
• NCBI Official Full Name: F-box only protein 44 isoform 1
• NCBI Official Synonym Full Names: F-box protein 44
• NCBI Official Symbol: FBXO44
• NCBI Official Synonym Symbols: FBG3; FBX30; FBX6A; Fbx44; Fbxo6a
• NCBI Protein Information: F-box only protein 44; F-box gene 3; F-box protein FBX30; F-box/G-domain protein 3
• UniProt Protein Name: F-box only protein 44
• Protein Family: F-box only protein
• UniProt Gene Name: FBXO44
• UniProt Synonym Gene Names: FBG3; FBX30; FBX44; FBX6A; FBXO6A
• UniProt Entry Name: FBX44_HUMAN

NCBI Description

This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of the ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbxs class. It is also a member of the NFB42 (neural F Box 42 kDa) family, similar to F-box only protein 2 and F-box only protein 6. Four alternatively spliced transcript variants encoding two distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

FBXO44: Substrate-recognition component of the SCF (SKP1-CUL1-F- box protein)-type E3 ubiquitin ligase complex. 2 isoforms of the human protein are produced by alternative splicing.

Chromosomal Location of Human Ortholog: 1p36.22

Cellular Component: SCF ubiquitin ligase complex

Molecular Function: protein binding