Rabbit anti-Human, Mouse FBXO24 Polyclonal Antibody | anti-FBXO24 antibody

FBXO24, ID (FBXO24, FBX24, F-box only protein 24)

Cat. Num. AAA6010191

• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot, Immunohistochemistry, Flow Cytometry, Functional Assay
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: FBXO24; Polyclonal Antibody; ID (FBXO24; FBX24; F-box only protein 24); Anti -FBXO24; anti-FBXO24 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Human, mouse
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

• Applicable Applications for anti-FBXO24 antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
• Application Notes: Suitable for use in Western Blot, Immunohistochemistry, Flow Cytometry, ELISA; Dilution: ELISA: 1:1,000; Western Blot: 1:100-500; Immunohistochemistry: 1:50-100; Flow Cytometry: 1:10-50
• Immunogen: FBXO24 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 118-145 amino acids from the Central region of human FBXO24.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(FBXO24 Antibody (Center) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the FBXO24 antibody detected the FBXO24 protein (arrow).)

Western Blot (WB)

(FBXO24 Antibody (Center) western blot analysis in mouse kidney tissue lysates (35ug/lane).This demonstrates the FBXO24 antibody detected the FBXO24 protein (arrow).)

Immunohistochemistry (IHC)

(FBXO24 antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the FBXO24 antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(FBXO24 Antibody (Center) flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-FBXO24 antibody

This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of the ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbxs class. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq].

Product Categories/Family for anti-FBXO24 antibody

Antibodies; Abs to Ubiquitin Ligases

NCBI and Uniprot Product Information

• NCBI GI #: 545687720
• NCBI GeneID: 101865732
• NCBI Accession #: NP_001270026.1
• NCBI GenBank Nucleotide #: NM_001283097.1
• UniProt Accession #: Q4R327
• Molecular Weight: 64,919 Da
• NCBI Official Full Name: F-box only protein 24
• NCBI Official Symbol: FBXO24
• NCBI Protein Information: F-box only protein 24
• UniProt Protein Name: F-box only protein 24
• Protein Family: F-box only protein
• UniProt Gene Name: FBXO24
• UniProt Entry Name: FBX24_MACFA

Uniprot Description

Function: Substrate-recognition component of the SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complex

By similarity.

Subunit structure: Directly interacts with SKP1 and CUL1

By similarity.

Sequence similarities: Contains 1 F-box domain.Contains 1 RCC1 repeat.