Rabbit anti-Human, Mouse FBP2 Polyclonal Antibody | anti-FBP2 antibody

FBP2, CT (Fructose-1,6-bisphosphatase Isozyme 2, FBPase 2, D-fructose-1,6-bisphosphate 1-phosphohydrolase 2) (MaxLight 550)

Cat. Num. AAA6477025

• Reactivity: Human, Mouse
• Applications: Western Blot
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: FBP2; Polyclonal Antibody; CT (Fructose-1; 6-bisphosphatase Isozyme 2; FBPase 2; D-fructose-1; 6-bisphosphate 1-phosphohydrolase 2) (MaxLight 550); anti-FBP2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human FBP2. Species Crossreactivity: mouse.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight550.

• Applicable Applications for anti-FBP2 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:50-1:100; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the C-terminal region of human FBP2 (KLH).
• Conjugate: MaxLight550
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight550 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of FBP2 Antibody (C-term) in mouse heart tissue lysates (35ug/lane). FBP2 (arrow) was detected using the purified Pab.)

Product Categories/Family for anti-FBP2 antibody

Antibodies; Enzymes; Phosphatase

References

1. Gizak, A., Proteins 72(1), 209-216 (2008). 2. Rakus, D., FEBS Lett. 579(25), 5577-5581 (2005).

NCBI and Uniprot Product Information

• NCBI GI #: 22907028
• NCBI GeneID: 8789
• NCBI Accession #: NP_003828
• NCBI GenBank Nucleotide #: NM_003837.3
• UniProt Accession #: O00757; Q17R39; Q6FI53
• Molecular Weight: 36,743 Da
• NCBI Official Full Name: fructose-1,6-bisphosphatase isozyme 2
• NCBI Official Synonym Full Names: fructose-bisphosphatase 2
• NCBI Official Symbol: FBP2
• NCBI Protein Information: fructose-1,6-bisphosphatase isozyme 2
• UniProt Protein Name: Fructose-1,6-bisphosphatase isozyme 2
• Protein Family: Fat body protein
• UniProt Gene Name: FBP2
• UniProt Synonym Gene Names: FBPase 2
• UniProt Entry Name: F16P2_HUMAN

NCBI Description

This gene encodes a gluconeogenesis regulatory enzyme which catalyzes the hydrolysis of fructose 1,6-bisphosphate to fructose 6-phosphate and inorganic phosphate. [provided by RefSeq, Jul 2008]

Uniprot Description

FBPase 2: a key enzyme of gluconeogenesis that catalyzes the hydrolysis of fructose 1,6-bisphosphate to fructose 6-phosphate, a precursor to glucose 6-phosphate. A regulator of glucose synthesis from non-carbohydrates. Two paralogs of this enzyme exists in humans: FBP1 is expressed in the liver and gluconeogenic tissues, and FBP2 in striated muscle. FBP2 is only isozyme expressed in striated muscle and it is widely expressed in cells that are nongluconeogenic. Both forms are inhibited allosterically by AMP, NAD and Ca2+. The muscle form is about 100-fold more sensitive to AMP and NAD, and about 1000-fold more sensitive to inhibition by Ca2+, the major activator of glycolysis in striated muscle. Forms an active complex with aldolase that binds to the sarcomeric Z-line, which is disrupted by elevated calcium levels, blocking glycogen synthesis during exercise. Forms homotetramers that are stabilized in the active state by divalent cations (Mg2+, Mn2+ , Co+2, or Zn2+). Its interaction with aldolase appears to desensitizes it to inhibition by AMP and, partially, by Ca2+. Up to 50% of lactate generated in striated muscle is converted to glycogen.

Protein type: Carbohydrate Metabolism - fructose and mannose; Carbohydrate Metabolism - pentose phosphate pathway; Carbohydrate Metabolism - glycolysis and gluconeogenesis; EC 3.1.3.11; Phosphatase (non-protein)

Chromosomal Location of Human Ortholog: 9q22.3

Cellular Component: Z disc; nucleus; cell junction; cytosol

Molecular Function: metal ion binding; fructose-bisphosphatase activity

Biological Process: dephosphorylation; carbohydrate metabolic process; glucose metabolic process; pathogenesis; fructose metabolic process; gluconeogenesis