Protocadherin Alpha 1 Recombinant Protein | PCDHa1 recombinant protein

Recombinant Protocadherin Alpha 1 (PCDHa1)

Cat. Num. AAA2033022

• Gene Names: PCDHA1; PCDH-ALPHA1
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 90%
• Synonyms: Protocadherin Alpha 1; Recombinant Protocadherin Alpha 1 (PCDHa1); PCDH-A1; PCDHa1 recombinant protein

• Host: E Coli
• Purity/Purification: > 90%
• Form/Format: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Sequence: QIHYSVPEEA KHGTFVGRIA QDLGLELTEL VPRLFRVASK DRGDLLEVNL QNGILFVNSR IDREELCGRS AECSIHLEVI VDRPLQVFHV EVKVKDINDN PPVFKGAEQR IFIPENRQLD SRFPLEGAVD ADIGANSLLT YTLSPTDYFS LKVETTDELS KSLSLELRKS LDREETPELQ LLLTATDGGK PELEGTVRLQ ITVLDVNDNA PLF
• Sequence Length: 950

• Applicable Applications for PCDHa1 recombinant protein: SDS-PAGE, Western (WB), ELISA (EIA), Immunoprecipitation (IP).
• Predicted Molecular Mass: 27.6kDa
• Usage: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Endotoxin Level: <1.0EU per 1 ug (determined by the LAL method)
• Expression System: Prokaryotic expression
• Fragment: Gln31~Phe243
• Organism Species: Mouse
• Tag: two N-terminal Tags, His-tag and T7-tag
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 9256582
• NCBI GeneID: 56147
• NCBI Accession #: NP_061723.1
• NCBI GenBank Nucleotide #: NM_018900.3
• Molecular Weight: 87,639 Da
• NCBI Official Full Name: protocadherin alpha-1 isoform 1
• NCBI Official Synonym Full Names: protocadherin alpha 1
• NCBI Official Symbol: PCDHA1
• NCBI Official Synonym Symbols: PCDH-ALPHA1
• NCBI Protein Information: protocadherin alpha-1
• UniProt Protein Name: Protocadherin alpha-1
• Protein Family: Protocadherin
• UniProt Gene Name: PCDHA1
• UniProt Synonym Gene Names: PCDH-alpha-1
• UniProt Entry Name: PCDA1_HUMAN

NCBI Description

This gene is a member of the protocadherin alpha gene cluster, one of three related gene clusters tandemly linked on chromosome five that demonstrate an unusual genomic organization similar to that of B-cell and T-cell receptor gene clusters. The alpha gene cluster is composed of 15 cadherin superfamily genes related to the mouse CNR genes and consists of 13 highly similar and 2 more distantly related coding sequences. The tandem array of 15 N-terminal exons, or variable exons, are followed by downstream C-terminal exons, or constant exons, which are shared by all genes in the cluster. The large, uninterrupted N-terminal exons each encode six cadherin ectodomains while the C-terminal exons encode the cytoplasmic domain. These neural cadherin-like cell adhesion proteins are integral plasma membrane proteins that most likely play a critical role in the establishment and function of specific cell-cell connections in the brain. Alternative splicing has been observed and additional variants have been suggested but their full-length nature has yet to be determined. [provided by RefSeq, Jul 2008]

Uniprot Description

PCDHA1: Potential calcium-dependent cell-adhesion protein. May be involved in the establishment and maintenance of specific neuronal connections in the brain. 3 isoforms of the human protein are produced by alternative splicing.

Chromosomal Location of Human Ortholog: 5q31

Cellular Component: endoplasmic reticulum; extracellular region; integral to plasma membrane; plasma membrane

Molecular Function: calcium ion binding

Biological Process: cell adhesion; homophilic cell adhesion; nervous system development