Western Blot (WB)
(Western Blot: Sample: Rat Liver Tissue.)
Rabbit anti-Rat Fatty Acid Transport Protein 5 (FATP5) Polyclonal Antibody | anti-FATP5 antibody
Polyclonal Antibody to Fatty Acid Transport Protein 5 (FATP5)
MRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTLEV LFQGPLGSEF- ETG KPGLLLTKIR KNQPFLGYRG SQDETKRKLV ANVRQVGDLY YNTGDVLALD QEGFFYFRDR LGDTFRWKGE NVSTREVEGV LSILDFLEEV NVYGVTVPGC EGKVGMAAVK LAPGKTFDGQ KLYQHVRSWL PAYATPHFIR IQDSLEITNT YKLVKSQLAR EGFDVGVIAD PLYILDNKAE TFRSLMPDVY QAVCEGTWKL
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Western Blot (WB)
(Western Blot: Sample: Rat Liver Tissue.)
Western Blot (WB)
(Western Blot: Sample: Rat Liver Tissue.)
Western Blot (WB)
(Western Blot: Sample: Recombinant FATP5, Rat.)
Western Blot (WB)
(Western Blot: Sample: Recombinant FATP5, Rat.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P. Samples: Rat Tissue))
Immunohistochemistry (IHC)
(DAB staining on IHC-P. Samples: Rat Tissue))
NCBI and Uniprot Product Information
NCBI Description
catalyzes the first of two steps in the conjugation of bile acids with the amino acids glycine and taurine [RGD, Feb 2006]
Uniprot Description
SLC27A5: Acyl-CoA synthetase involved in bile acid metabolism. Proposed to catalyze the first step in the conjugation of C24 bile acids (choloneates) to glycine and taurine before excretion into bile canaliculi by activating them to their CoA thioesters. Seems to activate secondary bile acids entering the liver from the enterohepatic circulation. In vitro, also activates 3-alpha,7- alpha,12-alpha-trihydroxy-5-beta-cholestanate (THCA), the C27 precursor of cholic acid deriving from the de novo synthesis from cholesterol. Belongs to the ATP-dependent AMP-binding enzyme family.
Protein type: EC 6.2.1.7; Endoplasmic reticulum; Ligase; Lipid Metabolism - primary bile acid biosynthesis; Membrane protein, integral; Membrane protein, multi-pass
Chromosomal Location of Human Ortholog: 1q12
Cellular Component: basal plasma membrane; endoplasmic reticulum; integral component of endoplasmic reticulum membrane; intracellular membrane-bound organelle; protein complex
Molecular Function: ATP binding; cholate-CoA ligase activity; fatty acid transmembrane transporter activity; long-chain-fatty-acid-CoA ligase activity; protein complex binding; very long-chain fatty acid-CoA ligase activity
Biological Process: bile acid biosynthetic process; bile acid metabolic process; fatty acid transport; ketone body biosynthetic process; long-chain fatty acid metabolic process; plasma membrane long-chain fatty acid transport; triacylglycerol mobilization; very long-chain fatty acid metabolic process
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Product Notes
The FATP5 slc27a5 (Catalog #AAA2004397) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Fatty Acid Transport Protein 5 (FATP5) reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Fatty Acid Transport Protein 5 (FATP5) can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB). Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the FATP5 slc27a5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with two N-terminal Tags, His-tag and its sequence is listed below. MRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTLEV LFQGPLGSEF - ETG KPGLLLTKIR KNQPFLGYRG SQDETKRKLV ANVRQVGDLY YNTGDVLALD QEGFFYFRDR LGDTFRWKGE NVSTREVEGV LSILDFLEEV NVYGVTVPGC EGKVGMAAVK LAPGKTFDGQ KLYQHVRSWL PAYATPHFIR IQDSLEITNT YKLVKSQLAR EGFDVGVIAD PLYILDNKAE TFRSLMPDVY QAVCEGTWKL. It is sometimes possible for the material contained within the vial of "Fatty Acid Transport Protein 5 (FATP5), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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