Western Blot (WB)
(Western Blot: Sample: Mouse Skin lysate; Primary Ab: 1ug/ml Rabbit Anti-Rat FABP5 Antibody;Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody;)
Rabbit anti-Rat Fatty Acid Binding Protein 5, Epidermal (FABP5) Polyclonal Antibody | anti-FABP5 antibody
Polyclonal Antibody to Fatty Acid Binding Protein 5, Epidermal (FABP5)
Gene Names
Fabp5; DA11; C-FABP; E-FABP
Reactivity
Rat
Applications
Immunocytochemistry, Immunohistochemistry, ELISA, Western Blot
Purity
Affinity Chromatography
Synonyms
Fatty Acid Binding Protein 5; Epidermal (FABP5); Polyclonal Antibody; Polyclonal Antibody to Fatty Acid Binding Protein 5; anti-FABP5 antibody
Host
Rabbit
Reactivity
Rat
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against FABP5. It has been selected for its ability to recognize FABP5 in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
Concentration
500ug/mL (varies by lot)
Sequence
Antigen: The target protein is fused with a His-tag and its sequence is listed below.
MGHHHHHHSGS-MASLKDLEGK WRLVESHGFE DYMKELGVGL ALRKMGAMAK PDCIITLDGN NLTVKTESTV KTTVFSCTLG EKFDETTADG RKTETVCTFT DGALVQHQKW EGKESTITRK LKDGKMVVEC VMNNAICTRV YEKVQ
MGHHHHHHSGS-MASLKDLEGK WRLVESHGFE DYMKELGVGL ALRKMGAMAK PDCIITLDGN NLTVKTESTV KTTVFSCTLG EKFDETTADG RKTETVCTFT DGALVQHQKW EGKESTITRK LKDGKMVVEC VMNNAICTRV YEKVQ
Applicable Applications for anti-FABP5 antibody
Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
Application Notes
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunogen
Recombinant FABP5 (Met1~Gln135) expressed in E.coli.
Cross Reactivity
Mouse, Rat
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2061568
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Western Blot: Sample: Mouse Skin lysate; Primary Ab: 1ug/ml Rabbit Anti-Rat FABP5 Antibody;Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody;)
Western Blot (WB)
(Western Blot: Sample: Mouse Skin lysate; Primary Ab: 1ug/ml Rabbit Anti-Rat FABP5 Antibody;Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody;)
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin-embedded lung tissue))
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin-embedded lung tissue))
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
15,059 Da
NCBI Official Full Name
fatty acid-binding protein, epidermal
NCBI Official Synonym Full Names
fatty acid binding protein 5
NCBI Official Symbol
Fabp5
NCBI Official Synonym Symbols
DA11; C-FABP; E-FABP
NCBI Protein Information
fatty acid-binding protein, epidermal
UniProt Protein Name
Fatty acid-binding protein, epidermal
Protein Family
UniProt Gene Name
Fabp5
UniProt Synonym Gene Names
C-FABP; E-FABP
NCBI Description
binds long chain fatty acids [RGD, Feb 2006]
Uniprot Description
FABP5: High specificity for fatty acids. Highest affinity for C18 chain length. Decreasing the chain length or introducing double bonds reduces the affinity. May be involved in keratinocyte differentiation. Belongs to the calycin superfamily. Fatty-acid binding protein (FABP) family.
Protein type: Lipid-binding
Chromosomal Location of Human Ortholog: 2q23
Cellular Component: cytoplasm; extracellular exosome
Molecular Function: fatty acid binding; transporter activity
Biological Process: glucose metabolic process; glucose transport; lipid metabolic process; phosphatidylcholine biosynthetic process; response to wounding; transport
Research Articles on FABP5
Similar Products
Product Notes
The FABP5 fabp5 (Catalog #AAA2004907) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Fatty Acid Binding Protein 5, Epidermal (FABP5) reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Fatty Acid Binding Protein 5, Epidermal (FABP5) can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB). Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the FABP5 fabp5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with a His-tag and its sequence is listed below. MGHHHHHHSG S-MASLKDLE GK WRLVESHGFE DYMKELGVGL ALRKMGAMAK PDCIITLDGN NLTVKTESTV KTTVFSCTLG EKFDETTADG RKTETVCTFT DGALVQHQKW EGKESTITRK LKDGKMVVEC VMNNAICTRV YEKVQ. It is sometimes possible for the material contained within the vial of "Fatty Acid Binding Protein 5, Epidermal (FABP5), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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