Mouse RAB11FIP3 Monoclonal Antibody | anti-RAB11FIP3 antibody

RAB11FIP3 (RAB11 Family Interacting Protein 3 (Class II), KIAA0665, Rab11-FIP3) (HRP)

Cat. Num. AAA6183367

• Gene Names: RAB11FIP3; CART1; FIP3-Rab11; Rab11-FIP3
• Applications: Western Blot
• Purity: Purified
• Synonyms: RAB11FIP3; Monoclonal Antibody; RAB11FIP3 (RAB11 Family Interacting Protein 3 (Class II); KIAA0665; Rab11-FIP3) (HRP); RAB11 Family Interacting Protein 3 (Class II); Rab11-FIP3; anti-RAB11FIP3 antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2a,k
• Clone Number: 6H6
• Specificity: Recognizes RAB11FIP3.
• Purity/Purification: Purified
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with horseradish peroxidase (HRP).
• Sequence Length: 756

• Applicable Applications for anti-RAB11FIP3 antibody: Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: RAB11FIP3 (NP_055515.1, 655aa-754aa) partial recombinant protein with GST tag. MW of the GST tag alone is 26kD.
• Immunogen Sequence: HSRARESELEQEVRRLKQDNRNLKEQNEELNGQIITLSIQGAKSLFSTAFSESLAAEISSVSRDELMEAIQKQEEINFRLQDYIDRIIVAIMETNPSILE
• Conjugate: HRP
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Testing Data

(Detection limit for recombinant GST tagged RAB11FIP3 is 0.03 ng/ml as a capture antibody.)

Product Categories/Family for anti-RAB11FIP3 antibody

Antibodies; GTPase; Rab; Ras; Rho Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 7662234
• NCBI GeneID: 9727
• NCBI Accession #: NP_055515.1
• NCBI GenBank Nucleotide #: NP_055515.1
• UniProt Accession #: O75154
• NCBI Official Full Name: rab11 family-interacting protein 3 isoform 1
• NCBI Official Synonym Full Names: RAB11 family interacting protein 3
• NCBI Official Symbol: RAB11FIP3
• NCBI Official Synonym Symbols: CART1; FIP3-Rab11; Rab11-FIP3
• NCBI Protein Information: rab11 family-interacting protein 3
• UniProt Protein Name: Rab11 family-interacting protein 3
• Protein Family: Rab11 family-interacting protein
• UniProt Gene Name: RAB11FIP3
• UniProt Synonym Gene Names: ARFO1; KIAA0665; FIP3-Rab11; Rab11-FIP3; Eferin
• UniProt Entry Name: RFIP3_HUMAN

NCBI Description

Proteins of the large Rab GTPase family (see RAB1A; MIM 179508) have regulatory roles in the formation, targeting, and fusion of intracellular transport vesicles. RAB11FIP3 is one of many proteins that interact with and regulate Rab GTPases (Hales et al., 2001 [PubMed 11495908]).[supplied by OMIM, Mar 2008]

Uniprot Description

RAB11FIP3: Acts as a regulator of endocytic traffic by participating in membrane delivery. Required for the abcission step in cytokinesis, possibly by acting as an 'address tag' delivering recycling endosome membranes to the cleavage furrow during late cytokinesis. Also required for the structural integrity of the endosomal recycling compartment during interphase. May play a role in breast cancer cell motility by regulating actin cytoskeleton. 3 isoforms of the human protein are produced by alternative splicing.

Chromosomal Location of Human Ortholog: 16p13.3

Cellular Component: nucleoplasm; centrosome; recycling endosome; intercellular bridge; intracellular membrane-bound organelle; recycling endosome membrane; microtubule organizing center; midbody; cytosol; endosome; cleavage furrow

Molecular Function: ADP-ribosylation factor binding; protein binding; protein homodimerization activity; calcium ion binding; Rab GTPase binding

Biological Process: vesicle-mediated transport; organelle organization and biogenesis; cytokinesis; endocytic recycling