Goat FAPP2/PLEKHA8 Polyclonal Antibody | anti-PLEKHA8 antibody

Goat anti-FAPP2/PLEKHA8 Antibody

Cat. Num. AAA420752

• Gene Names: PLEKHA8; FAPP2
• Reactivity: Tested: Human; Expected from sequence similarity: Human
• Applications: ELISA, Immunohistochemistry
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Synonyms: FAPP2/PLEKHA8; Polyclonal Antibody; Goat anti-FAPP2/PLEKHA8 Antibody; PLEKHA8; FAPP2; phosphoinositol 4-phosphate adaptor protein-2; MGC3358; pleckstrin homology domain containing; family A (phosphoinositide binding specific) member 8; PLEKHA8 antibody; FAPP2 antibody; phosphoinositol 4-phosphate adaptor protein-2 antibody; MGC3358 antibody; family A (phosphoinositide binding specific) member 8 antibody; FAPP2 / PLEKHA8; anti-PLEKHA8 antibody

• Host: Goat
• Reactivity: Tested: Human; Expected from sequence similarity: Human
• Clonality: Polyclonal
• Purity/Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Form/Format: Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Concentration: 100ug specific antibody in 200ul (varies by lot)
• Sequence: DIQTALRNPTENT
• Sequence Length: 440

• Applicable Applications for anti-PLEKHA8 antibody: Peptide ELISA (EIA), Immunohistochemistry (IHC)
• Application Notes: Peptide ELISA: Antibody detection limit dilution 1: 64000.; Immunohistochemistry: In paraffin embedded Human Placenta shows strong staining of cytoplasm in the margins of villi. Recommended concentration, 2-4ug/ml.; Western Blot: Preliminary experiments gave no signal but low background in Human Kidney and Testis lysates at up to 1ug/ml.
• Immunogen: Peptide with sequence C-DIQTALRNPTENT, from the C Terminus of the protein sequence according to NP_116028.1.
• Epitope: C Terminus
• Preparation and Storage: Aliquot and store at -20 degree C. Minimize freezing and thawing.

Immunohistochemistry (IHC)

((2.5ug/ml) staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)

NCBI and Uniprot Product Information

• NCBI GI #: 14249174
• NCBI GeneID: 84725
• NCBI Accession #: NP_116028.1
• NCBI GenBank Nucleotide #: NM_032639.3
• Molecular Weight: 49,462 Da
• NCBI Official Full Name: pleckstrin homology domain-containing family A member 8 isoform 3
• NCBI Official Synonym Full Names: pleckstrin homology domain containing A8
• NCBI Official Symbol: PLEKHA8
• NCBI Official Synonym Symbols: FAPP2
• NCBI Protein Information: pleckstrin homology domain-containing family A member 8
• UniProt Protein Name: Pleckstrin homology domain-containing family A member 8
• Protein Family: Pleckstrin homology domain-containing family
• UniProt Gene Name: PLEKHA8
• UniProt Synonym Gene Names: FAPP2; PH domain-containing family A member 8; FAPP-2; Phosphoinositol 4-phosphate adapter protein 2; hFAPP2
• UniProt Entry Name: PKHA8_HUMAN

Uniprot Description

PLEKHA8: Cargo transport protein that is required for apical transport from the Golgi complex. Transports AQP2 from the trans- Golgi network (TGN) to sites of AQP2 phosphorylation. Mediates the non-vesicular transport of glucosylceramide (GlcCer) from the trans-Golgi network (TGN) to the plasma membrane and plays a pivotal role in the synthesis of complex glycosphingolipids. Binding of both phosphatidylinositol 4-phosphate (PIP) and ARF1 are essential for the GlcCer transfer ability. Also required for primary cilium formation, possibly by being involved in the transport of raft lipids to the apical membrane, and for membrane tubulation. 3 isoforms of the human protein are produced by alternative splicing.

Chromosomal Location of Human Ortholog: 7p14.3

Cellular Component: membrane; trans-Golgi network

Molecular Function: glycolipid binding; glycolipid transporter activity

Biological Process: glycolipid transport; lipid transport; protein transport