Rabbit anti-Human FAP1 Polyclonal Antibody | anti-FAP1 antibody

FAP1 Polyclonal Antibody

Cat. Num. AAA3012677

• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry
• Purity: ProA affinity purified
• Synonyms: FAP1; Polyclonal Antibody; FAP1 Polyclonal Antibody; FAPalpha; SIMP; Seprase; FAP; APO1/CD95 (Fas) associated phosphatase; DKFZp686J1497; FAP 1; Fas associated phosphatase 1; Fas associated protein tyrosine phosphatase 1; HGNC: 9646; hPTPE1; Phosphatase rip; PNP 1; PNP1; Protein tyrosine phosphatase 1 Fas associated; protein tyrosine phosphatase 1E; Protein tyrosine phosphatase non receptor type 13; Protein tyrosine phosphatase nonreceptor type 13; Protein tyrosine phosphatase PTPL1; protein tyrosine phosphatase; non-receptor type 13 (APO-1/CD95 (Fas)-associated phosphatase); PTP BAS; PTP BL; PTP E1; PTP1E; PTPL 1; PTPL1; PTPLE; PTPN 13; PTPN13; Tyrosine protein phosphatase non receptor type 13; anti-FAP1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: FAP1 polyclonal antibody detects endogenous levels of FAP1 protein.
• Purity/Purification: ProA affinity purified
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 760

• Applicable Applications for anti-FAP1 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500-1:1,000; IHC: 1:50-1:200
• Immunogen: recombinant protein
• Preparation and Storage: Store at 4 degree C after thawing.; Aliquot store at -20 degree C or -80 degree C.; Avoid repeated freeze/thaw cycles.

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-FAP1 antibody. Counter stained with hematoxylin.)

Related Product Information for anti-FAP1 antibody

FAP (fibroblast activation protein) is a cell surface glycoprotein and serine protease that is expressed primarily in fetal mesenchymal tissues and epithelial cancer fibroblasts. In cancer, FAP functions to promote cellular proliferation. In embryonic development, FAP functions to remodel developing tissues. FAP acts as an integral membrane gelatinase composed of N-glycosylated proteolytically inactive subunits. FAP expression on chondrocyte membranes is upregulated by the combination of the cytokines IL-1 and OSM and has been shown to increase in osteoarthritic patients. This expression is co-localized with MMP-1and MMP-13 as well as CD44 (variants v3 and v7/8). Mice that lack all copies of the FAP gene have been found to be fertile and to have developmental defects or change in cancer susceptibility.

NCBI and Uniprot Product Information

• NCBI GI #: 16933540
• NCBI Accession #: NP_004451.2
• NCBI GenBank Nucleotide #: NP_004451.2
• UniProt Accession #: Q12884
• Molecular Weight: 95kDa
• NCBI Official Full Name: prolyl endopeptidase FAP isoform 1
• UniProt Protein Name: Seprase
• Protein Family: Fap1 adhesin
• UniProt Gene Name: FAP
• UniProt Entry Name: SEPR_HUMAN

Uniprot Description

FAP: In association with DPP4 is involved in the pericellular proteolysis of the extracellular matrix (ECM), the migration and invasion of endothelial cells into the ECM. May have a role in tissue remodeling during development and wound healing, and may contribute to invasiveness in malignant cancers. Belongs to the peptidase S9B family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.4.14.5; Protease; EC 3.4.21.26; Membrane protein, integral

Chromosomal Location of Human Ortholog: 2q23

Cellular Component: extracellular space; focal adhesion; cell surface; lamellipodium; apical part of cell; cytoplasm; plasma membrane; integral to membrane

Molecular Function: integrin binding; protein dimerization activity; peptidase activity; protein binding; protein homodimerization activity; protease binding; dipeptidyl-peptidase activity; serine-type peptidase activity; metalloendopeptidase activity; serine-type endopeptidase activity; endopeptidase activity

Biological Process: proteolysis involved in cellular protein catabolic process; endothelial cell migration; angiogenesis; proteolysis; cell adhesion; regulation of fibrinolysis