Western Blot (WB)
(Figure: WB: 20ug of ES Cell total extract was probed with (0.5ug/ml).)
Rabbit REST Polyclonal Antibody | anti-REST antibody
REST (Repressor Element RE-1 Silencing Transcription Factor, NRSF, Neuronal Restricted Silencing Factor) (MaxLight 490)
Gene Names
REST; XBR; NRSF
Reactivity
Human, Mouse, Rat
Applications
Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
REST; Polyclonal Antibody; REST (Repressor Element RE-1 Silencing Transcription Factor; NRSF; Neuronal Restricted Silencing Factor) (MaxLight 490); anti-REST antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human REST, Mr 120-200kD. Species Crossreactivity: mouse and rat
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight490.
Applicable Applications for anti-REST antibody
Western Blot (WB)
Application Notes
Applications are based on unconjugated antibody.
WB: 1:500-1:2000. ES cell total extract was resolved by electrophoresis, transferred to nitrocellulose and probed with. Proteins were visualized using an IgG (HRP) goat anti-rabbit and a chemiluminescence detection system.
WB: 1:500-1:2000. ES cell total extract was resolved by electrophoresis, transferred to nitrocellulose and probed with. Proteins were visualized using an IgG (HRP) goat anti-rabbit and a chemiluminescence detection system.
Immunogen
GST fusion protein corresponding to residues 801-1097 of human REST (NM_005612).
Conjugate
MaxLight490
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight490 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Western Blot (WB)
(Figure: WB: 20ug of ES Cell total extract was probed with (0.5ug/ml).)
Western Blot (WB)
(Figure: WB: 20ug of ES Cell total extract was probed with (0.5ug/ml).)
Product Categories/Family for anti-REST antibody
References
1. Ballas, N., et.al., Neuron 31: 353-365, 2001. 2. Schoenherr, C.J., et.al., Science 267: 1360-1363, 1995. 3. Chong, J.A., et.al., Cell 80: 949-957, 1995.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
1097
NCBI Official Full Name
RE1-silencing transcription factor
NCBI Official Synonym Full Names
RE1-silencing transcription factor
NCBI Official Symbol
REST
NCBI Official Synonym Symbols
XBR; NRSF
NCBI Protein Information
RE1-silencing transcription factor; repressor binding to the X2 box; neural-restrictive silencer factor; neuron restrictive silencer factor
UniProt Protein Name
RE1-silencing transcription factor
Protein Family
UniProt Gene Name
REST
UniProt Synonym Gene Names
NRSF; XBR
UniProt Entry Name
REST_HUMAN
Similar Products
Product Notes
The REST rest (Catalog #AAA6497273) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The REST (Repressor Element RE-1 Silencing Transcription Factor, NRSF, Neuronal Restricted Silencing Factor) (MaxLight 490) reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's REST can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Applications are based on unconjugated antibody. WB: 1:500-1:2000. ES cell total extract was resolved by electrophoresis, transferred to nitrocellulose and probed with. Proteins were visualized using an IgG (HRP) goat anti-rabbit and a chemiluminescence detection system. Researchers should empirically determine the suitability of the REST rest for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "REST, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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