Rabbit FAP ALPHA Polyclonal Antibody | anti-FAP antibody

RABBIT ANTI HUMAN FAP ALPHA

Cat. Num. AAA222803

• Gene Names: FAP; FAPA; SIMP; DPPIV
• Applications: Immunohistochemistry
• Synonyms: FAP ALPHA; Polyclonal Antibody; RABBIT ANTI HUMAN FAP ALPHA; anti-FAP antibody

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: This item detects fibroblast activation protein alpha (FAP alpha), a type II membrane protein belonging to the serine protease family. FAP alpha is upregulated on activated fibroblasts in response to wounding, and may also be involved in epithelial-mesenchymal interactions during development and tissue repair. ; ; Active FAP alpha is a 170kD homodimeric protein which can also exist as a soluble form. FAP alpha can also form heterodimeric complexes with CD26 (DPP4), and these proteins may function co-ordinately to modulate the growth, differentiation, adhesion and metastasis of tumour cells.
• Form/Format: Purified; Purified IgG - liquid
• Concentration: IgG concentration 0.5 mg/ml (varies by lot)
• Sequence Length: 735

• Applicable Applications for anti-FAP antibody: Immunohistology Paraffin*
• Application Notes: Immunohistology - Paraffin: Maximum Dilution: 10 ug/ml; Application Note: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.
• Perservative Stabilisers: 0.09% Sodium Azide (NaN3)
• Immunogen: Synthetic peptide: extracellular domain of human FAP alpha, conjugated to keyhole limpet hemocyanin.; Histology Positive Control Tissue
• Antiserum Preparation: Antisera to FAP alpha were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
• Target Species: Human
• Preparation and Storage: Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 18 months from date of despatch.

Testing Data #1

(Staining of formalin fixed, paraffin embedded human uterus with Rabbit anti Human FAB alpha (MBS222803))

Related Product Information for anti-FAP antibody

MBS222803 detects fibroblast activation protein alpha (FAP alpha), a type II membrane protein belonging to the serine protease family. FAP alpha is upregulated on activated fibroblasts in response to wounding, and may also be involved in epithelial-mesenchymal interactions during development and tissue repair. Active FAP alpha is a 170kD homodimeric protein which can also exist as a soluble form. FAP alpha can also form heterodimeric complexes with CD26 (DPP4), and these proteins may function co-ordinately to modulate the growth, differentiation, adhesion and metastasis of tumour cells.

NCBI and Uniprot Product Information

• NCBI GI #: 620597362
• NCBI GeneID: 2191
• NCBI Accession #: NP_001278736.1
• NCBI GenBank Nucleotide #: NM_001291807.1
• UniProt Accession #: Q12884; O00199; Q53TP5; Q86Z29; Q99998; Q9UID4
• Molecular Weight: 26,954 Da
• NCBI Official Full Name: prolyl endopeptidase FAP isoform 2
• NCBI Official Synonym Full Names: fibroblast activation protein, alpha
• NCBI Official Symbol: FAP
• NCBI Official Synonym Symbols: FAPA; SIMP; DPPIV
• NCBI Protein Information: prolyl endopeptidase FAP; 170 kDa melanoma membrane-bound gelatinase; FAPalpha; dipeptidyl peptidase FAP; gelatine degradation protease FAP; integral membrane serine protease; post-proline cleaving enzyme; seprase; serine integral membrane protease; surface-expressed protease
• UniProt Protein Name: Prolyl endopeptidase FAP
• Protein Family: Prolyl endopeptidase
• UniProt Gene Name: FAP
• UniProt Entry Name: SEPR_HUMAN

NCBI Description

The protein encoded by this gene is a homodimeric integral membrane gelatinase belonging to the serine protease family. It is selectively expressed in reactive stromal fibroblasts of epithelial cancers, granulation tissue of healing wounds, and malignant cells of bone and soft tissue sarcomas. This protein is thought to be involved in the control of fibroblast growth or epithelial-mesenchymal interactions during development, tissue repair, and epithelial carcinogenesis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2014]

Uniprot Description

FAP: In association with DPP4 is involved in the pericellular proteolysis of the extracellular matrix (ECM), the migration and invasion of endothelial cells into the ECM. May have a role in tissue remodeling during development and wound healing, and may contribute to invasiveness in malignant cancers. Belongs to the peptidase S9B family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.4.21.26; EC 3.4.14.5; Protease; Membrane protein, integral

Chromosomal Location of Human Ortholog: 2q23

Cellular Component: extracellular space; cell surface; focal adhesion; lamellipodium; apical part of cell; cytoplasm; plasma membrane; integral to membrane

Molecular Function: protein dimerization activity; peptidase activity; integrin binding; protein binding; protein homodimerization activity; protease binding; dipeptidyl-peptidase activity; serine-type peptidase activity; serine-type endopeptidase activity; metalloendopeptidase activity; endopeptidase activity

Biological Process: proteolysis involved in cellular protein catabolic process; endothelial cell migration; angiogenesis; proteolysis; cell adhesion; regulation of fibrinolysis