FAK Polyclonal Antibody | anti-FAK antibody

Anti-FAK Antibody

Cat. Num. AAA178148

• Gene Names: PTK2; FAK; FADK; FAK1; FRNK; PPP1R71; p125FAK; pp125FAK
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: Immunogen Affinity Purified
• Synonyms: FAK; Polyclonal Antibody; Anti-FAK Antibody; Focal adhesion kinase 1; FADK 1; FADK; FAK 1; FAK related non kinase polypeptide; FAK1; FAK1_HUMAN; Focal adhesion kinase related nonkinase; FRNK; p125FAK; pp125FAK; PPP1R71; Protein phosphatase 1 regulatory subunit 71; Protein tyrosine kinase 2; Protein Tyrosine Kinase Cytoplasmic; Protein-tyrosine kinase 2; PTK 2; PTK2; PTK2 protein tyrosine kinase 2 antibody; PTK2 protein tyrosine kinase 2; anti-FAK antibody

• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Purity/Purification: Immunogen Affinity Purified
• Form/Format: Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
• Sequence Length: 1065

• Applicable Applications for anti-FAK antibody: Western Blot (WB)
• Application Notes: Western Blot Concentration: 0.1-0.5ug/ml; Tested Species: Human, Mouse, Rat; ; Tested Species: In-house tested species with postive results.; ; Other applications have not been tested.; Optimal dilutions should be determined by end users.
• Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human PTK2 (1024-1052aa AHALAVDAKNLLDVIDQARLKMLGQTRPH), identical to the related mouse and rat sequences.
• Ig Type: Rabbit IgG
• Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Preparation and Storage: At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.

Western Blot (WB)

(Anti- FAK Picoband antibody, MBS178148, Western blottingAll lanes: Anti FAK (MBS178148) at 0.5ug/mlLane 1: Rat Lung Tissue Lysate at 50ugLane 2: Mouse Lung Tissue Lysate at 50ugLane 3: Rat Brain Tissue Lysate at 50ugLane 4: U20S Whole Cell Lysate at 40ugLane 5: HELA Whole Cell Lysate at 40ugLane 6: NIH3T3 Whole Cell Lysate at 40ugPredicted bind size: 125KDObserved bind size: 125KD )

Related Product Information for anti-FAK antibody

Background: PTK2 protein tyrosine kinase 2 (PTK2), also known as Focal Adhesion Kinase (FAK), is a protein that, in humans, is encoded by the PTK2 gene. This gene encodes a cytoplasmic protein tyrosine kinase which is found concentrated in the focal adhesions that form between cells growing in the presence of extracellular matrix constituents. The encoded protein is a member of the FAK subfamily of protein tyrosine kinases but lacks significant sequence similarity to kinases from other subfamilies. Activation of this gene may be an important early step in cell growth and intracellular signal transduction pathways triggered in response to certain neural peptides or to cell interactions with the extracellular matrix. Several transcript variants encoding different isoforms have been found for this gene, but the full-length natures of only three of them have been determined.

References

1. Beggs, H. E., Schahin-Reed, D., Zang, K., Goebbels, S., Nave, K.-A., Gorski, J., Jones, K. R., Sretavan, D., Reichardt, L. F.FAK deficiency in cells contributing to the basal lamina results in cortical abnormalities resembling congenital muscular dystrophies.Neuron 40: 501-514, 2003. 2. Li, W., Lee, J., Vikis, H. G., Lee, S.-H., Liu, G., Aurandt, J., Shen, T.-L., Fearon, E. R., Guan, J.-L., Han, M., Rao, Y., Hong, K., Guan, K.-L.Activation of FAK and Src are receptor-proximal events required for netrin signaling.Nature Neurosci. 7: 1213-1221, 2004.

NCBI and Uniprot Product Information

• NCBI GI #: 313851044
• NCBI GeneID: 5747
• NCBI Accession #: NP_001186578.1
• NCBI GenBank Nucleotide #: NM_001199649.1
• UniProt Accession #: Q05397; Q14291; Q8IYN9; Q9UD85; B4E2N6; F5H4S4; J3QT16
• NCBI Official Full Name: focal adhesion kinase 1 isoform c
• NCBI Official Synonym Full Names: protein tyrosine kinase 2
• NCBI Official Symbol: PTK2
• NCBI Official Synonym Symbols: FAK; FADK; FAK1; FRNK; PPP1R71; p125FAK; pp125FAK
• NCBI Protein Information: focal adhesion kinase 1
• UniProt Protein Name: Focal adhesion kinase 1
• UniProt Gene Name: PTK2
• UniProt Synonym Gene Names: FAK; FAK1; FADK 1; FRNK; PPP1R71
• UniProt Entry Name: FAK1_HUMAN

NCBI Description

This gene encodes a cytoplasmic protein tyrosine kinase which is found concentrated in the focal adhesions that form between cells growing in the presence of extracellular matrix constituents. The encoded protein is a member of the FAK subfamily of protein tyrosine kinases but lacks significant sequence similarity to kinases from other subfamilies. Activation of this gene may be an important early step in cell growth and intracellular signal transduction pathways triggered in response to certain neural peptides or to cell interactions with the extracellular matrix. Several transcript variants encoding different isoforms have been found for this gene, but the full-length natures of only four of them have been determined. [provided by RefSeq, Oct 2015]

Uniprot Description

FAK: a tyrosine kinase of the FAK family required for cell migration and contact-dependent survival signaling. Activated by tyrosine-phosphorylation in response to either integrin clustering induced by cell adhesion or antibody cross-linking, or via G-protein coupled receptor (GPCR) occupancy by ligands such as bombesin or lysophosphatidic acid, or via LDL receptor occupancy. Downstream of integrins and Src, upstream of Ras/MAPK. Localizes to focal adhesions that form between cells growing in the presence of extracellular matrix constituents. Interacts with CAS family members and with GIT1, SORBS1 and BCAR3. Interacts with Shb. Required for full Ras transformation of fibroblasts. Increased expression in breast and other cancers, related to chromosome 8q amplification. Overexpression and activation associated with increased migration, invasion and progression of ovarian cancer, and with progression in hepatocellular carcinoma, thyroid cancer, and acute myelogenous leukemia. siRNA increases chemosensitivity of pancreatic adenocarcinoma xenografts. Inhibitor: ISI15421 (antisense). Four splice-variant isoforms have been observed.

Protein type: Protein kinase, tyrosine (non-receptor); Kinase, protein; Protein kinase, TK; EC 2.7.10.2; TK group; Fak family

Chromosomal Location of Human Ortholog: 8q24.3

Cellular Component: apical plasma membrane; cell cortex; cytoplasm; cytoskeleton; cytosol; extrinsic to internal side of plasma membrane; focal adhesion; lamellipodium; microtubule organizing center; nucleus; plasma membrane; stress fiber

Molecular Function: actin binding; ATP binding; JUN kinase binding; non-membrane spanning protein tyrosine kinase activity; protein binding; protein kinase activity; protein kinase binding; protein-tyrosine kinase activity; Ras guanyl-nucleotide exchange factor activity; receptor binding; SH2 domain binding; signal transducer activity

Biological Process: angiogenesis; axon guidance; cell motility involved in cell locomotion; cell structure disassembly during apoptosis; central nervous system neuron axonogenesis; embryonic development; endothelial cell migration; ephrin receptor signaling pathway; epidermal growth factor receptor signaling pathway; establishment of cell polarity; establishment of nucleus localization; extracellular matrix organization and biogenesis; heart morphogenesis; innate immune response; integrin-mediated signaling pathway; MAPKKK cascade; microtubule cytoskeleton organization and biogenesis; negative regulation of apoptosis; negative regulation of axonogenesis; negative regulation of cell-cell adhesion; negative regulation of organ growth; negative regulation of synaptogenesis; neuron migration; peptidyl-tyrosine phosphorylation; placenta development; positive regulation of cell migration; positive regulation of cell proliferation; positive regulation of GTPase activity; positive regulation of phosphoinositide 3-kinase activity; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of protein amino acid phosphorylation; positive regulation of protein kinase activity; positive regulation of protein kinase B signaling cascade; protein amino acid autophosphorylation; regulation of cell adhesion mediated by integrin; regulation of cell proliferation; regulation of cell shape; regulation of cytoskeleton organization and biogenesis; regulation of focal adhesion formation; regulation of GTPase activity; regulation of osteoblast differentiation; regulation of protein amino acid phosphorylation; signal complex assembly; transforming growth factor beta receptor signaling pathway; vascular endothelial growth factor receptor signaling pathway; vasculogenesis